Identification of nitric oxide synthase in human and bovine oviduct

Rosselli, Marinella; Dubey, Raghvendra K.; Macas, Ervin; Fink, David M.; Lauper, Urs; Keller, Paul J.; Imthurn, Bruno

doi:10.1093/molehr/2.8.607

Cited by 67 publications

(57 citation statements)

References 24 publications

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“…NADPH diaphorase was demontrated mainly in the oviduct epithelium of man, rat and pig (Bryant et al 1995, Ekerhovd et al 1999, Gawronska et al 2000, and eNOS protein was consistently present in oviduct epithelial cells of different species (Bryant et al 1995, Rosselli et al 1996, Ekerhovd et al 1997, Gawronska et al 2000. In the present study, we clearly demonstrate the presence of both eNOS and iNOS in BOEC.…”

Section: Discussionsupporting

confidence: 69%

“…Recently, NOS have been identified in the human, bovine and rat oviduct (Bryant et al 1995, Rosselli et al 1996, Ekerhovd et al 1997. Nevertheless, more comprehensive analyses could help to bridge lack of congruency for different species (Chatterjee et al 1996, Gawronska et al 2000 with respect to cellular localisation, regions of the oviduct and cycle-dependent regulation.…”

Section: Introductionmentioning

confidence: 99%

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Region-specific expression of nitric oxide synthases in the bovine oviduct during the oestrous cycle and in vitro

Ulbrich¹,

Rehfeld²,

Bauersachs³

et al. 2006

Journal of Endocrinology

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Nitric oxide synthases (NOS) account for the endogenous production of nitric oxide (NO), a small and permeable bioreactive molecule. NO is known to act as a paracrine mediator during various processes associated with female reproduction. In the present study, the mRNA expression of the endothelial (eNOS) and inducible (iNOS) NO synthases were examined in bovine oviduct epithelial cells (BOEC) during the oestrous cycle. In addition, eNOS and iNOS mRNA and protein were localised by in situ hybridisation and immunocytochemistry respectively. Furthermore, the effects of exogenously applied oestradiol-17 and progesterone on NOS mRNA regulation were studied in a suspension culture of BOEC. The eNOS mRNA abundance was low around ovulation (day 0) and increased significantly until pro-oestrus (day 18) in the ampulla. Immunoreactive protein of eNOS was detected predominantly in endothelial cells as well as in secretory oviduct epithelial cells at pro-oestrus. The iNOS mRNA concentration was significantly reduced in the isthmus at pro-oestrus (day 18) and oestrus (day 0) compared with persistently high levels in the ampulla. By in situ hybridisation, specific iNOS transcripts were additionally demonstrated in the oviduct epithelium. Immunoreactive iNOS protein was localised in secretory epithelial cells as well as in the lamina muscularis. The in vitro stimulation showed that both NOS were stimulated by progesterone, but not by oestradiol-17 . The region-specific modulated expression of eNOS and iNOS provides evidence for an involvement of endogenously produced NO in the regulation of oviductal functions.

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Section: Discussionsupporting

confidence: 69%

Section: Introductionmentioning

confidence: 99%

Region-specific expression of nitric oxide synthases in the bovine oviduct during the oestrous cycle and in vitro

Ulbrich¹,

Rehfeld²,

Bauersachs³

et al. 2006

Journal of Endocrinology

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“…NO plays an important role during fertilization in the oviduct. iNOS and eNOS expression regulates the transport of gametes and embryos in addition to providing a specific microenvironment that facilitates the fertilization process [35]. In the ovary, increases in NO correlate with increases in estrogen, regulates follicular development [36] and ovulation [37].…”

Section: Discussionmentioning

confidence: 99%

Regulation of the nitric oxide pathway genes by tetrahydrofurandiols: Microarray analysis of MCF-7 human breast cancer cells

et al. 2008

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THF-diols (9,12-oxy-10,13-dihydroxyoctadecanoic and 10,13-oxy-9,12-dihydroxyoctadecanoic acids) are endocrine disrupters in rats and mitogens in breast cancer cells. Microarray analyses and real-time PCR analyses on RNA from THF-treated MCF-7 cells revealed a number of genes (Caveolin 1, Heat Shock Protein 90α and 90β, vascular endothelial growth factor, ATPase, Ca++ transporting, ubiquitous) in the Nitric Oxide Pathway (NOP) were targets for THF-diols. Chromatin immunoprecipitation studies suggest THF-diols modify of histone H4 acetylation at the Caveolin 1 promoter via an epigenetic mechanism. These findings are consistent with the well-known involvement of NOP genes in cell proliferation and sexual behavior.

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“…The endocrine and paracrine control mechanism of oviductal functions involves several factors including ovarian steroids (Wollenhaupt et al 2002), prostaglandin (PG) F2 and PGE2 (Harper 1988), nitric oxide (NO) (Rosselli et al 1996, Perez Martinez et al 1997, growth factors (Makarevich & Sirotkin 1997) and cytokines (Wijayagunawardane et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Leptin receptor expression and in vitro leptin actions on prostaglandin release and nitric oxide synthase activity in the rabbit oviduct

Zerani¹,

Boiti²,

Dall’Aglio³

et al. 2005

Journal of Endocrinology

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In this study, we have examined the presence and the distribution of receptors for leptin (Ob-R) in the oviduct of rabbits, and the effects of leptin on the release of prostaglandin (PG) F2 and PGE2 and on the activity of nitric oxide (NO) synthase (NOS) by oviducts cultured in vitro. Rabbits were killed during the follicular phase and the oviducts were incubated in vitro with leptin, PGF2 , PGE2, NO donor and inhibitors of NOS and cyclooxigenase (COX). Using immunohistochemistry, Ob-Rlike positive reaction was observed only in the cytoplasm of secretory cells, having stronger intensity in the infundibulum and ampulla tracts than in the isthmus. Both leptin and NO donor inhibited PGE2 release, whereas they enhanced PGF2 release; NOS inhibitor alone or with leptin increased PGE2 and decreased PGF2 production; NOS activity was enhanced by leptin, while PGs did not affect this enzyme. This study suggests that the oviduct could be a potential target for endocrine regulation by leptin, whose circulating levels may act as a metabolic signal modulating oviductal PG release through mediation of the NOS/NO system.

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Identification of nitric oxide synthase in human and bovine oviduct

Cited by 67 publications

References 24 publications

Region-specific expression of nitric oxide synthases in the bovine oviduct during the oestrous cycle and in vitro

Region-specific expression of nitric oxide synthases in the bovine oviduct during the oestrous cycle and in vitro

Regulation of the nitric oxide pathway genes by tetrahydrofurandiols: Microarray analysis of MCF-7 human breast cancer cells

Leptin receptor expression and in vitro leptin actions on prostaglandin release and nitric oxide synthase activity in the rabbit oviduct

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