Identification of Novel Interaction between Annexin A2 and Keratin 17

Abstract

Background: Expression of EGFR, K17, and AnxA2 are correlated in cancer settings. Results: AnxA2 and K17 physically interact and undergo reciprocal regulation controlled in part by EGFR signaling. Conclusion: K17 serves as a regulator of the signaling pathway involving AnxA2, whereas AnxA2 regulates K17 stability. Significance: This study demonstrates a novel interaction and reciprocal regulation between AnxA2 and K17.

A Conserved Rod Domain Phosphotyrosine That Is Targeted by the Phosphatase PTP1B Promotes Keratin 8 Protein Insolubility and Filament Organization*

“…In the present study, we demonstrated that EGFR activation is a negative regulator of K8/K18 tyrosine phosphorylation in the insoluble compartment. Because K8 phosphorylation at Tyr-267 decreases K8 solubility, our findings align with a recent study showing that longer EGFR activation (1 h) increases the levels of soluble K17 (38).…”

Keratins 17 and 19 expression as prognostic markers in oral squamous cell carcinoma

“…Keratins are also involved in protein synthesis and epithelial cell growth (Kim et al, 2006), signaling (Alam et al, 2011), organelle transport (Planko et al, 2007), and cell mobility and proliferation (Chung et al, 2012). There are 54 genes that encode type I (acidic) and type II (basic) keratins, which form heterodimers and show tissue-specific expression (Flitney et al, 2009).…”

Regulation of C-X-C chemokine gene expression by keratin 17 and hnRNP K in skin tumor keratinocytes

“…An NS shRNA has also been described previously (Chung et al, 2012). A SCR shRNA was designed by scrambling the KRT17 targeting sequence at position 387 (Table 2) and cloned in pSuper.…”

“…) for a Krt17 null allele were isolated and cultured as described previously (Chung et al, 2012 ) for the Krt17 allele were isolated by mincing ears into fine pieces and subjecting them to 0.25% Trypsin overnight. After vigorous vortexing, free keratinocytes were isolated as described previously (Chung et al, 2012) and cultured in Cnt-57 medium (CELLnTEC) before harvesting.…”

“…After vigorous vortexing, free keratinocytes were isolated as described previously (Chung et al, 2012) and cultured in Cnt-57 medium (CELLnTEC) before harvesting. For TPA stimulation, cells previously incubated for 18 h in 0.1% FBS-containing medium were treated with either DMSO (vehicle control) or 200 nM TPA for the indicated time periods.…”