Identification of Paracoccus Solventivorans sp. as Sulphate Oxidizing Bacteria Isolated from Palm Oil Sludge

Abstract

The emission of hydrogen sulphide (H2S) from industrial processes causesodour nuisance to the surrounding community. Thus, the ability of sulphur oxidizing bacteria could eliminate H2S by changing it into non-odorous elemental sulphur or sulphate. In this study, the isolate bacteria of Paracoccus solventivorans ATCC 700252 demonstrated pH reduction from pH 8 to about pH 5.27 with sulphate content production of454.0 g/ml. The growth of Paracoccus sp. was increased using sulphur as elemental carbon and oxygen as electron acceptor. The optimum temperature and pH of Paracoccus sp. were in the range of 30°C to 40°C and pH 7.00 to pH 8.00, respectively. The utilization of Paracoccus sp. will create a potential application for H2S removal and preserve the environment from further odour pollution.

Characterization of sulfur-oxidizing bacteria isolated from mustard (Brassica juncea L.) rhizosphere having the capability of improving sulfur and nitrogen uptake

“…In another study, SOB (both autotrophs and heterotrophs) were isolated and screened from sulfur water having sulfur oxidation capacity in water (Koul et al, 2018). Recently, many research have been working on the isolation of SOB from different sources with having applications in divergent areas (Borse et al, 2021;Dou et al, 2022;Huynh et al, 2021;Jadhav et al, 2022;Joseph et al, 2021;Nadella et al, 2019;Shinde et al, 2022;Tajarudin et al, 2020;Updegraff et al, 2022;Xie et al, 2021), etc. The initial pH of broth decreased from 8.0 to acidic pH due to the oxidation of sodium thiosulfate to sulfate ions.…”

“…Kambam et al (2015) and Ashraf et al (2018) also screened SOB on the basis of their potential to reduce the pH of the growth medium from 7.8 to ≤5.0 within 15 days of incubation and to oxidize elemental sulfur (S°), and Devi with her team screened rhizobacteria (Indira Devi et al, 2011). Tajarudin et al (2020) also reported the isolation of SOB having the ability to reduce the pH of the medium. In our study, the increase in sulfate production was due to the reaction of BaCl 2 and sulfate in the medium produced by isolates, which results in the formation of barium sulfate.…”

“…In another study, Tajarudin and co‐workers also studied sulfur‐oxidizing isolates. They also characterize bacteria on the basis of biochemical and molecular characteristics (Tajarudin et al, 2020). Nadella et al (2019) also studied their isolates for many biochemical tests like thiosulfate utilization, catalase, indole and oxidase production, nitrite and nitrate reduction, etc.…”

“…The morphological parameters examined include colony characteristics, Gram's reaction, colony shape and colour, the shape of cells and arrangement of the cell. The biochemical characters such as catalase production, Indole production, oxidase test, citrate utilization test, methyl red test, Voges‐Proskauer reaction, acid production, H 2 S production, cellulose hydrolysis, starch hydrolysis and motility test were studied using standard culture testing methods as followed by Nadella et al (2019) and Tajarudin et al (2020).…”

“…As we know, micro‐organisms have been proved as a potential game changer in many fields including crop production and improvement of the environment. Recently, many attempts have been made to isolate and characterize SOB from many sources like aquaculture farm soil (Nadella et al, 2019), palm oil sludge (Tajarudin et al, 2020), fresh water sediment (Xie et al, 2021), processed wastewater from food industries (Borse et al, 2021), extensive shrimp ponds (Huynh et al, 2021; Joseph et al, 2021), coastal regions (Shinde et al, 2022), anchialine sinkhole of river (Updegraff et al, 2022), wastewater (Dou et al, 2022), etc. In this study, SOB were isolated from the mustard fields and their efficiency was checked in terms of N and S uptake in wheat and mustard.…”