Identification of potential candidate genes involved in the sex determination cascade in an aquatic firefly, Sclerotia aquatilis (Coleoptera, Lampyridae)

Nguantad, Sarintip; Chumnanpuen, Pramote; Thancharoen, Anchana; Vongsangnak, Wanwipa; Sriboonlert, Ajaraporn

doi:10.1016/j.ygeno.2020.01.025

Cited by 9 publications

(6 citation statements)

References 106 publications

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“…It suggests that Hctra-2 may play a potential regulatory role in gonadal development of H. cumingii. But in many other species, tra-2 has a high expression pattern of ovary, such as P. chinensis (Li et al, 2012), Aedes albopictus (Li et al, 2019), E. chinensis (Luo et al, 2017), and aquatic firefly (Sclerotia aquatilis; Nguantad et al, 2020). The different expression patterns of tra-2 indicate that its regulation of gonadal development is different among species.…”

Section: Discussionmentioning

confidence: 99%

Identification and Functional Analysis of the Sex-Determiner Transformer-2 Homologue in the Freshwater Pearl Mussel, Hyriopsis cumingii

Wang

et al. 2021

Front. Physiol.

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Transformer-2 (Tra-2) is an upstream regulatory element of the sex regulation mechanism in insects and plays a critical role in sex formation. To understand the role of tra-2 in Hyriopsis cumingii, the full-length Hctra-2 (1867 bp) was obtained from the gonads, and sequence alignment with other species showed that HCTRA-2 protein had a highly conserved RRM domain. Phylogenetic analysis showed that the HCTRA-2 protein was a close relative to of the mollusks TRA-2 protein. The qRT-PCR of tissue-specific expression pattern showed that the Hctra-2 was abundant in gonads, and the expression in testes was higher than that in ovaries (p < 0.01). It suggests that Hctra-2 may play a potential regulatory role in gonadal development of H. cumingii. In the early gonadal development, the Hctra-2 expression was the highest on the third day after fertilization and increased slightly from 4 months to 5 months, which may be related to the embryonic sex determination and early gonadal development. In situ hybridization showed that Hctra-2 mRNA signals were present in both male and female gonads. After silencing Hctra-2 by RNAi, the expression levels of Hcfem-1b and Hcdmrt were changed. It is speculated that there may be a certain relationship between them, which plays an important role in the sex regulation of H. cumingii. Our research will help to deepen our understanding of the shellfish sex determination mechanisms.

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Section: Discussionmentioning

confidence: 99%

Identification and Functional Analysis of the Sex-Determiner Transformer-2 Homologue in the Freshwater Pearl Mussel, Hyriopsis cumingii

Wang

et al. 2021

Front. Physiol.

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“…In this context, discussion of successful reproduction strategies and genetic factors governing sex determination is pertinent. Gonadal transcriptome analysis of insect species led to the identification of candidate genes involved in sex-determination/differentiation and reproduction, thereby providing a scientific basis for exploring sex-related economic traits associated with disease resistance and the overall health of the organism [ 91 ]. In the transcriptome of R. ferrugineus, 25 genes were annotated as relevant to reproduction, including five vitellogenin transcripts; however, only a single vitellogenin gene was expressed [ 54 ], consistent with reports of a single vitellogenin gene in other coleopteran species such as T. molitor [ 92 ] , Anthonomus grandis [ 93 ] , Octodonta nipae [ 94 ] , and Colaphellus bowringi [ 95 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of the endangered dung beetle Copris tripartitus (Coleoptera: Scarabaeidae) and characterization of genes associated to immunity, growth, and reproduction

et al. 2023

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Background Dung beetles recycle organic matter through the decomposition of feces and support ecological balance. However, these insects are threatened by the indiscriminate use of agrochemicals and habitat destruction. Copris tripartitus Waterhouse (Coleoptera: Scarabaeidae), a dung beetle, is listed as a class-II Korean endangered species. Although the genetic diversity of C. tripartitus populations has been investigated through analysis of mitochondrial genes, genomic resources for this species remain limited. In this study, we analyzed the transcriptome of C. tripartitus to elucidate functions related to growth, immunity and reproduction for the purpose of informed conservation planning. Results The transcriptome of C. tripartitus was generated using next-generation Illumina sequencing and assembled de novo using a Trinity-based platform. In total, 98.59% of the raw sequence reads were processed as clean reads. These reads were assembled into 151,177 contigs, 101,352 transcripts, and 25,106 unigenes. A total of 23,450 unigenes (93.40%) were annotated to at least one database. The largest proportion of unigenes (92.76%) were annotated to the locally curated PANM-DB. A maximum of 5,512 unigenes had homologous sequences in Tribolium castaneum. Gene Ontology (GO) analysis revealed a maximum of 5,174 unigenes in the Molecular function category. Further, in Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, a total of 462 enzymes were associated with established biological pathways. Based on sequence homology to known proteins in PANM-DB, representative immunity, growth, and reproduction-related genes were screened. Potential immunity-related genes were categorized into pattern recognition receptors (PRRs), the Toll-like receptor signaling pathway, the MyD88- dependent pathway, endogenous ligands, immune effectors, antimicrobial peptides, apoptosis, and adaptation-related transcripts. Among PRRs, we conducted detailed in silico characterization of TLR-2, CTL, and PGRP_SC2-like. Repetitive elements such as long terminal repeats, short interspersed nuclear elements, long interspersed nuclear elements and DNA elements were enriched in the unigene sequences. A total of 1,493 SSRs were identified among all unigenes of C. tripartitus. Conclusions This study provides a comprehensive resource for analysis of the genomic topography of the beetle C. tripartitus. The data presented here clarify the fitness phenotypes of this species in the wild and provide insight to support informed conservation planning.

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“…The regulatory hierarchy upstream of these splicing events is F I G U R E 1 A phylogenetic tree showcasing the conserved nature of fru and dsx across different orders within Arthropoda (Bertossa et al, 2009;Just et al, 2023;Kientega et al, 2022;A.-M. Li et al, 2022;P. Li et al, 2017;Nguantad et al, 2020;Pomerantz & Hoy, 2015;Pomerantz & Hoy, 2015;Thaijongrak et al, 2022). As discussed in the text, conservation of these genes does not necessarily mean that they play the same role in each species that they do in Diptera.…”

Section: Gene Regulatory Events Upstream Of Dsx/frumentioning

confidence: 99%

Let's talk about sex: Mechanisms of neural sexual differentiation in Bilateria

Roggenbuck,

Hall,

Hanson

et al. 2024

WIREs Mechanisms of Disease

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In multicellular organisms, sexed gonads have evolved that facilitate release of sperm versus eggs, and bilaterian animals purposefully combine their gametes via mating behaviors. Distinct neural circuits have evolved that control these physically different mating events for animals producing eggs from ovaries versus sperm from testis. In this review, we will describe the developmental mechanisms that sexually differentiate neural circuits across three major clades of bilaterian animals–Ecdysozoa, Deuterosomia, and Lophotrochozoa. While many of the mechanisms inducing somatic and neuronal sex differentiation across these diverse organisms are clade‐specific rather than evolutionarily conserved, we develop a common framework for considering the developmental logic of these events and the types of neuronal differences that produce sex‐differentiated behaviors.This article is categorized under: Congenital Diseases > Stem Cells and Development Neurological Diseases > Stem Cells and Development

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Identification of potential candidate genes involved in the sex determination cascade in an aquatic firefly, Sclerotia aquatilis (Coleoptera, Lampyridae)

Cited by 9 publications

References 106 publications

Identification and Functional Analysis of the Sex-Determiner Transformer-2 Homologue in the Freshwater Pearl Mussel, Hyriopsis cumingii

Identification and Functional Analysis of the Sex-Determiner Transformer-2 Homologue in the Freshwater Pearl Mussel, Hyriopsis cumingii

Transcriptome analysis of the endangered dung beetle Copris tripartitus (Coleoptera: Scarabaeidae) and characterization of genes associated to immunity, growth, and reproduction

Let's talk about sex: Mechanisms of neural sexual differentiation in Bilateria

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