Identification of potential genes and miRNAs associated with sepsis based on microarray analysis

Abstract

Sepsis is a type of systemic inflammatory response syndrome caused by infection. The present study aimed to examine key genes and microRNAs (miRNAs) involved in the pathogenesis of sepsis. The GSE13205 microarray dataset, downloaded from the Gene Expression Omnibus was analyzed using bioinformatics tools, and included muscle biopsy specimens of 13 patients with sepsis and eight healthy controls. The differentially expressed genes (DEGs) in samples from patients with sepsis were identified using the Linear Models for Microarray package in R language. Using the Database for Annotation, Visualization and Integration Discovery tool, functional and pathway enrichment analyses were performed to examine the potential functions of the DEGs. The protein-protein interaction (PPI) network was constructed with the DEGs using the Search Tool for the Retrieval of Interacting Genes, and the network topology was analyzed using CytoNCA. Subsequently, MCODE in Cytoscape was used to identify modules in the PPI network. Finally, the integrated regulatory network was constructed based on the DEGs, miRNAs and transcription factors (TFs). A total of 259 upregulated DEGs (MYC and BYSL) and 204 downregulated DEGs were identified in the patients with sepsis. NOP14, NOP2, AATF, GTPBP4, BYSL and TRMT6 were key genes in the MCODE module. In the integrated DEG-miRNA-TF regulatory network, hsa-miR-150 (target gene MYLK3) and 21 TFs, comprising 14 upregulated DEGs (including MYC) and seven downregulated DEGs, were identified. The results suggested that NOP14, NOP2, AATF, GTPBP4, BYSL, MYC, MYLK3 and miR-150 may be involved in the pathogenesis of sepsis.

Up‐regulation of microRNA‐574 attenuates lipopolysaccharide‐ or cecal ligation and puncture‐induced sepsis associated with acute lung injury

“…MiRNAs, which are a class of non‐coding nucleotide single‐stranded small‐molecule RNAs, participate in various biological processes such as cell proliferation, differentiation and apoptosis . In recent years, studies have found that miRNA could be used as a new biomarker in the diagnosis of sepsis . Researcher found that serum miR‐574 could be used as a predictor of prognosis in patients with sepsis .…”

“…12 In recent years, studies have found that miRNA could be used as a new biomarker in the diagnosis of sepsis. [13][14][15] Researcher found that serum miR-574 could be used as a predictor of prognosis in patients with sepsis. 16,17 However, the dynamic expression of miRNA-574 in sepsis has not been reported.…”

The lncRNA CCAT2 Rs6983267 G Variant Contributes to Increased Sepsis Susceptibility in a Southern Chinese Population

“…24 A number of studies have found that MYC participates in the pathogenesis of sepsis. [25][26][27] Together, these findings suggest that CCAT2 may be involved in the pathology of sepsis. Recent studies have reported that CCAT2 gene polymorphisms are associated with susceptibility to various diseases, such as recurrent miscarriage, endometrial carcinoma and colorectal cancer, [28][29][30] and further research has shown that the rs6983267 polymorphism in the CCAT2 contributes to increases in MYC expression.…”

Investigations into the pharmacological action of vioprolide A on inflammatory and angiogenic processes in endothelial cells

“…[321,322] Interestingly, besides being connected to cancer cell functions, NOP14 has also been shown to constitute a key gene in sepsis and may thus be associated with systemic inflammation. [323] 5.…”

“…NOP14 has been reported as potential marker of sepsis, indicating a general involvement in the immune response. [323] Nevertheless, the role of NOP14 in the response towards inflammatory stimuli has not been elucidated, yet. Considering that vioprolide A exerts strong anti-inflammatory actions and targets NOP14 in endothelial cells, a potential connection between these two events is likely.…”