Identification of Proteins and Peptide Biomarkers for Detecting Banned Processed Animal Proteins (PAPs) in Meat and Bone Meal by Mass Spectrometry

Marbaix, Hélène; Budinger, Dimitri; Dieu, Marc; Fumière, Olivier; Gillard, Nathalie; Delahaut, Philippe; Mauro, Sergio; Raes, Martine

doi:10.1021/acs.jafc.6b00064

Cited by 40 publications

(49 citation statements)

References 27 publications

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“…Tricine-SDS-PAGE gels (4% spacer gel and 16% separating gel) were used to resolve the peptide extracts. Proteins were separated by SDS-PAGE (5% spacer gel and 10% separating gel) [ 26 , 27 ]. The results were analyzed by Quantity One version 4.6.2 (Bio-Rad Technical Service Department, Oakland, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Identification and Antithrombotic Activity of Peptides from Blue Mussel (Mytilus edulis) Protein

Qiao

Wang

et al. 2018

IJMS

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The blue mussel (Mytilus edulis) reportedly contains many bioactive components of nutritional value. Water-, salt- and acid-soluble M. edulis protein fractions were obtained and the proteins were trypsinized. The resultant peptides were analyzed by ultra-performance liquid chromatography quadrupole time of flight tandem mass spectrometry (UPLC-Q-TOF-MS/MS). 387 unique peptides were identified that matched 81 precursor proteins. Molecular mass distributions of the proteins and peptides were analyzed by sodium dodecyl sulfate-polyacryl amide gel electrophoresis (SDS-PAGE). The differences between the three protein samples were studied by Venn diagram of peptide and protein compositions. Toxicity, allergic and antithrombotic activity of peptides was predicted using database website and molecular docking respectively. The antithrombotic activity of enzymatic hydrolysate from water-, salt- and acid-soluble M. edulis protein were 40.17%, 85.74%, 82.00% at 5 mg/mL, respectively. Active mechanism of antithrombotic peptide (ELEDSLDSER) was also research about amino acid binding sites and interaction, simultaneously.

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Section: Methodsmentioning

confidence: 99%

Identification and Antithrombotic Activity of Peptides from Blue Mussel (Mytilus edulis) Protein

Qiao

Wang

et al. 2018

IJMS

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“…One notable issue relates to the erroneous detection of pig blood products (RIKILT, unpublished results). It appeared to be difficult to detect bovine specific markers based on peptide sequences, detectable at 1%, in porcine and vegetal matrices (Marbaix et al 2016). In the framework of a project with publicprivate cooperation RIKILT tried to develop a poultry specific antibody with no success until now.…”

Section: Protein Based Methods: Antibodiesmentioning

confidence: 99%

Bridging legal requirements and analytical methods: a review of monitoring opportunities of animal proteins in feed

Raamsdonk¹,

Prins²,

Meijer³

et al. 2019

Food Additives & Contaminants: Part A

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Availability and safety of food ranks among the basic requirements for human beings. The importance of the food producing sector, inclusive of feed manufacturing, demands a high level of regulation and control. This paper will present and discuss the relationships in the triangle of legislation, the background of hazards with a biological nature, and opportunities for monitoring methods, most notable for prion-based diseases as primary issue. The European Union legislation for prevention of prion-based diseases since 2000 is presented and discussed. The definitions and circumscriptions of groups of species will be analysed in the view biological classification and evolutionary relationships. The state of the art of monitoring methods is presented and discussed. Methods based on visual markers (microscopy), DNA-based methods (PCR), protein-based methods (ELISA, mass spectroscopy, proteomics), near infrared oriented methods and combinations thereof are being evaluated. It is argued that the use in legislation of non-homogeneous groups of species in a biological sense will hamper the optimal design of monitoring methods. Proper definitions are considered to act as bridges between legal demands and suitable analytical methods for effective monitoring. Definitions including specified groups of species instead of single species are more effective for monitoring in a range of cases. Besides the desire of precise circumscription of animal groups targeted by legislation, processed products need well defined definitions as well. Most notable examples are blood versus blood products, and hydrolysis of several types of material. The WISE principle for harmonising the design of legislation and of analytical methods is discussed. This principle includes the elements Witful (reasonable legal principles), Indicative (clear limits between prohibition and authorisation), Societal demands (public health, environment, economy), and Enforceable (presence of suited monitoring methods) in order to promote a balanced effort for reaching the desired level of safety in the food production chain.

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“…Premixed Protein Standard (44.3–200 kDa) of Takara Co. Ltd. (Dalian, China) was used for finding the relative molecular weight. The samples were mixed with 10 µL of electrophoretic loading buffer and heated for 5 min in a boiling water bath [ 26 , 27 ]. Different samples were separated by centrifugation at 8000 rpm for 15 min.…”

Section: Methodsmentioning

confidence: 99%

Identification and In Silico Prediction of Anticoagulant Peptides from the Enzymatic Hydrolysates of Mytilus edulis Proteins

Qiao

Chen

et al. 2018

IJMS

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Mytilus edulis is a typical marine bivalve mollusk. Many kinds of bioactive components with nutritional and pharmaceutical activities in Mytilus edulis were reported. In this study, eight different parts of Mytilus edulis tissues, i.e., the foot, byssus, pedal retractor muscle, mantle, gill, adductor muscle, viscera, and other parts, were separated and the proteins from these tissues were prepared. A total of 277 unique peptides from the hydrolysates of different proteins were identified by UPLC-Q-TOF-MS/MS, and the molecular weight distribution of the peptides in different tissues was investigated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The bioactivity of the peptides was predicted through the Peptide Ranker database and molecular docking. Moreover, the peptides from the adductor muscle were chosen to do the active validation of anticoagulant activity. The active mechanism of three peptides from the adductor muscle, VQQELEDAEERADSAEGSLQK, RMEADIAAMQSDLDDALNGQR, and AAFLLGVNSNDLLK, were analyzed by Discovery Studio 2017, which also explained the anticoagulant activity of the hydrolysates of proteins from adductor muscle. This study optimized a screening and identification method of bioactive peptides from enzymatic hydrolysates of different tissues in Mytilus edulis.

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Identification of Proteins and Peptide Biomarkers for Detecting Banned Processed Animal Proteins (PAPs) in Meat and Bone Meal by Mass Spectrometry

Cited by 40 publications

References 27 publications

Identification and Antithrombotic Activity of Peptides from Blue Mussel (Mytilus edulis) Protein

Identification and Antithrombotic Activity of Peptides from Blue Mussel (Mytilus edulis) Protein

Bridging legal requirements and analytical methods: a review of monitoring opportunities of animal proteins in feed

Identification and In Silico Prediction of Anticoagulant Peptides from the Enzymatic Hydrolysates of Mytilus edulis Proteins

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