Identification of putative quantitative trait loci associated with a flavonoid related to resistance to cabbage seedpod weevil (Ceutorhynchus obstrictus) in canola derived from an intergeneric cross, Sinapis alba × Brassica napus

Abstract

Kaempferol 3- O -sinapoyl-sophoroside 7- O -glucoside was putatively identified as the major component of a characteristic HPLC peak previously correlated with the reduction of cabbage seedpod weevil larval infestation in a novel canola genotype. The cabbage seedpod weevil (Ceutorhynchus obstrictus [Marsham]) (CSPW) is a serious pest of brassicaceous oilseed crops such as canola in both Europe and more recently in North America. At present, the only control strategy against CSPW is the application of insecticides. As an alternative more environmentally-friendly control strategy, we developed novel canola germplasm resistant to weevil attack through introgression of Sinapis alba DNA into Brassica napus by making the wide cross followed by embryo rescue and backcrossing to the B. napus parent. We have previously characterized resistant canola lines by metabolic profiling and were able to correlate reduction of larval infestation to the presence of a characteristic HPLC peak. In this study, we have putatively identified the major component in the peak using mass spectrometry as kaempferol 3-O-sinapoyl-sophoroside 7-O-glucoside (KSSG). We have also identified quantitative trait loci (QTL) associated with this HPLC peak in a mapping population consisting of more than 200 individual doubled haploid (DH) lines derived from a cross between CSW428 (the resistant parent) and SC030686 (the susceptible parent). This QTL accounted for approximately 9.5 % of the phenotypic variation in KSSG content. The observation that only one QTL was identified as surpassing the LOD threshold of 3.0 suggests that both parents may possess the positive alleles for other QTL that have not been detected in our study. This finding also indicates a complex regulatory mechanism for KSSG levels and provides an appropriate explanation for the large transgressive segregation observed in the DH lines of the QTL mapping population.

Draft genome of multiple resistance donor plant Sinapis alba: An insight into SSRs, annotations and phylogenetics

“…S. alba could be a potential donor of genetic resistance for biotic disease, environmental stresses, and some other agronomic traits to close relatives such as B. juncea, B. napus and other brassica crops [12,26,27]. Much research has been focused on this wild plant for its medicinal values because of the presence of anti-bacterial, antioxidant, and anti-cancer chemicals [13].…”

De novo Transcriptome Analysis of Sinapis alba in Revealing the Glucosinolate and Phytochelatin Pathways

“…Sinapis alba , known as yellow mustard or white mustard, is an important cruciferous crop widely used as food condiments in the world (Hemingway, 1995 ). It has many desirable agronomic traits, such as tolerance or resistance to drought, disease, pests, and pod-shattering (Thompson, 1963 ; Bodnaryk and Lamb, 1991 ; Brown et al, 1997 ; Lee et al, 2014 ), making it an attractive resource for oil crop breeding (Tian et al, 2014 ). The genetically close relationship and the ease of forming hybrids between S. alba and Brassica plants make it a potential donor of resistant and other agronomic traits to Brassica napus and other Brassica crops (Brown et al, 1997 ; Jiang et al, 2013 ; Lee et al, 2014 ).…”

Evaluation of nine genotypes of oilseed rape (Brassica napus L.) for larval infestation and performance of rape stem weevil (Ceutorhynchus napi Gyll.)

“…[56] and crucifer-specific phytoalexins are discussed for their importance for infestation by D . radicum [57].…”