Identification of quantitative trait loci for blast resistance in BC2F3 and BC2F5 advanced backcross families of rice

Harun, Abdul Rahim; Bhuiyan, Md. Atiqur Rahman; Lim, Li Sze; Sabu, K. K.; Abdullah, Shahrum; Azhar, Mohamad; Wickneswari, R.

doi:10.4238/2012.september.12.11

Cited by 13 publications

(6 citation statements)

References 30 publications

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“…With the growing population, the demand made on the supply of rice is high. Therefore, breeding programs are directed towards increasing yield in response to both biotic and abiotic stresses [14,15]. One such stress that affects yield is drought.…”

Section: Introductionmentioning

confidence: 99%

Drought Response in Rice: The miRNA Story

Nadarajah

Kumar

2019

IJMS

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As a semi-aquatic plant, rice requires water for proper growth, development, and orientation of physiological processes. Stress is induced at the cellular and molecular level when rice is exposed to drought or periods of low water availability. Plants have existing defense mechanisms in planta that respond to stress. In this review we examine the role played by miRNAs in the regulation and control of drought stress in rice through a summary of molecular studies conducted on miRNAs with emphasis on their contribution to drought regulatory networks in comparison to other plant systems. The interaction between miRNAs, target genes, transcription factors and their respective roles in drought-induced stresses is elaborated. The cross talk involved in controlling drought stress responses through the up and down regulation of targets encoding regulatory and functional proteins is highlighted. The information contained herein can further be explored to identify targets for crop improvement in the future.

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Section: Introductionmentioning

confidence: 99%

Drought Response in Rice: The miRNA Story

Nadarajah

Kumar

2019

IJMS

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“…Several inheritance studies have been reported that have used Japanese races (e.g., Kiyosawa, 1981;He and Shen, 1990). In Malaysia, inheritance studies have been performed using local isolates (Rahim et al, 2012), including BC 2 F 3 and BC 2 F 5 advanced backcrosses (Oryza rufipogon x MR219). Four dominant genes at different loci were identified against the most virulent pathotype of M. oryzae Tanweer et al, 2015a).…”

Section: Introductionmentioning

confidence: 99%

Genetic analysis and identification of SSR markers associated with rice blast disease in a BC<sub>2</sub>F<sub>1</sub> backcross population

et al. 2017

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ABSTRACT. Rice (Oryza sativa L.) blast disease is one of the most destructive rice diseases in the world. The fungal pathogen, Magnaporthe oryzae, is the causal agent of rice blast disease. Development of 2 N. Hasan et al. Genetics and Molecular Research 16 (1): gmr16019280 resistant cultivars is the most preferred method to achieve sustainable rice production. However, the effectiveness of resistant cultivars is hindered by the genetic plasticity of the pathogen genome. Therefore, information on genetic resistance and virulence stability are vital to increase our understanding of the molecular basis of blast disease resistance. The present study set out to elucidate the resistance pattern and identify potential simple sequence repeat markers linked with rice blast disease. A backcross population (BC 2 F 1 ), derived from crossing MR264 and Pongsu Seribu 2 (PS2), was developed using marker-assisted backcross breeding. Twelve microsatellite markers carrying the blast resistance gene clearly demonstrated a polymorphic pattern between both parental lines. Among these, two markers, RM206 and RM5961, located on chromosome 11 exhibited the expected 1:1 testcross ratio in the BC 2 F 1 population. The 195 BC 2 F 1 plants inoculated against M. oryzae pathotype P7.2 showed a significantly different distribution in the backcrossed generation and followed Mendelian segregation based on a single-gene model. This indicates that blast resistance in PS2 is governed by a single dominant gene, which is linked to RM206 and RM5961 on chromosome 11. The findings presented in this study could be useful for future blast resistance studies in rice breeding programs.

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“…Numerous studies have identified blast QTL from susceptible lines under greenhouse conditions (Xu et al, 2004; Lopez‐Gerena, 2006). The resistance QTL qBLR10‐1 and qBLR10‐2 colocalized with qBL10.1 (Rahim et al, 2012) and OsMPK6 (Hu et al, 2008), suggesting that they may be involved in similar blast‐resistant responses. It is also possible that differences in resistance QTL detected in the greenhouse was a result of confounding environmental effects, including the presence of different blast races in the field.…”

Section: Discussionmentioning

confidence: 98%

Confirming and Identifying New Loci for Rice Blast Disease Resistance using Magnaporthe oryzae Field Isolates in the US

et al. 2015

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Quantitative trait loci (QTL) play important roles in controlling rice blast disease. In the present study, 10 field isolates of the races IA1, IB1, IB17, and IC1 of US rice blast fungus Magnaporthe oryzae collected in 1996 and 2009 were used to identify blast resistance QTL with a recombinant inbred line (RIL) population consisting of 227 F7 individuals derived from the cross of rice (Oryza sativa L.) cultivars Lemont and Jasmine 85. Jasmine 85 is an indica cultivar that is moderately resistant, and Lemont is a tropical japonica cultivar susceptible to rice blast in greenhouse inoculation. Disease reactions of the parents and RILs were evaluated under greenhouse conditions. A total of six resistance QTL, qBLR8, qBLR10‐1, qBLR10‐2, qBLR10‐3, qBLR12‐1, and qBLR12‐2, were identified on chromosomes 8, 10, and 12, respectively. Phenotypic variation, conditioned by these six resistance QTL, ranged from 5.37 to 39.18%. Among them, qBLR12‐1 and qBLR12‐2 provided the strongest resistance to the newest isolates of the most virulent race IA1 of M. oryzae. Three of these resistance QTL have been identified using different blast isolates in a previous study. qBLR10‐1, qBLR10‐2, and qBLR10‐3 have not been previously found in this cross. These confirmed and new resistance QTL will be useful for the development of rice cultivars with improved effective resistance to rice blast via a marker‐assisted selection (MAS) approach.

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Identification of quantitative trait loci for blast resistance in BC2F3 and BC2F5 advanced backcross families of rice

Cited by 13 publications

References 30 publications

Drought Response in Rice: The miRNA Story

Drought Response in Rice: The miRNA Story

Genetic analysis and identification of SSR markers associated with rice blast disease in a BC<sub>2</sub>F<sub>1</sub> backcross population

Confirming and Identifying New Loci for Rice Blast Disease Resistance using Magnaporthe oryzae Field Isolates in the US

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