Identification of regulatory elements in 16S rRNA gene of Acinetobacter species isolated from water sample

Srivastava, Shalini; Singh, Vijai; Kumar, Vipin; Verma, Praveen Chandra; Srivastava, Rajeev; Basu, Vaishali; Gupta, Vijayta; Rawat, Anurag

doi:10.6026/97320630003173

Cited by 40 publications

(27 citation statements)

References 6 publications

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“…Figures 1, 2, and 3 show these genes by gel electrophoresis under UV light visualization. (1,2,3,4,5,6,9,10,11,12,13,14,17,18,19) represent positive results while line (7,8,15,16) show negative results . (1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19) represent positive results.…”

Section: Results:-mentioning

confidence: 99%

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Molecular Detection of Gyra and Parc Genes in Multidrug Resistant Acinetobacter Baumannii Isolated From Nosocomial Infections in Children.

Abbood¹,

Manhal²

2016

IJAR

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A. baumannii, multi-drug-resistant, gyrA and parC This study was conducted in Children Welfare Teaching Hospital, Baghdad, Iraq in order to detect gyrA and parC genes in multidrug resistant Acinetobacter baumannii isolated from nosocomial infections in children. A total of 50 child patients were selected according to study criteria. Different specimens obtained from child patients and hospital environment were tested by culture and antimicrobial sensitivity according to standard bacteriological and molecular techniques. Blood stream infections comprised the highest number and percentages within suspected nosocomial infections, 21 (42%) of paediatric patients were detected with early onset sepsis (EOS), and 19 (38%) were with late onset sepsis (LOS). Forty A. baumannii isolates were identified at species level by using VITEK® 2 Compact and 16s rRNA. Oligonucleotide primers sequences were used for PCR amplification of 16s rRNA , gyrA and parC of Acinetobacter baumannii. All of A. baumannii isolates were multidrug resistant (MDR) pathogens. Resistance gyrA and parC genes were detected in 58% and 100% of study isolates, respectively. Nosocomial sepsis in neonates were still at high-frequency in child patients. The vast majority of A. baumannii isolates were multidrug resistant and harboring gyrA and parC resistance genes. Considerable efforts are needed for choosing antimicrobial drugs and combination therapy. Further studies are required for genotyping by sequencing of problematic resistant A. baumannii isolates in our hospitals.

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Section: Results:-mentioning

confidence: 99%

“…Line M: 100bp ladder. Line(1,2,3,4,5,6,7,8,9,13,14,15,16) represent positive results, while line(10,11,12,17,18,19) show negative results.…”

mentioning

confidence: 99%

Molecular Detection of Gyra and Parc Genes in Multidrug Resistant Acinetobacter Baumannii Isolated From Nosocomial Infections in Children.

Abbood¹,

Manhal²

2016

IJAR

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“…This technique may provide information in terms of the community structure, diversity, evolution and taxonomy of microbial flora in the tin mining area. The sequencing information may also explain the discrepancy between phenotypic identification and the antibiotic susceptibility of the various isolates (Srivastava et al, 2008). These novel isolates will allow the exploration of new antibacterial agent and industrial enzyme with enhance prowess.…”

Section: Resultsmentioning

confidence: 99%

Biological screening of microbes isolated from soil of ex-tin mining land in Kampar Area

Ong¹

2011

Afr. J. Microbiol. Res.

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Soil samples from ex-tin mining area located in proximity to Universiti Tunku Abdul Rahman (UTAR), Kampar campus was collected and cultured on nutrient agar plates. The morphological characteristics including basic staining, Gram staining and endospore staining of five selected isolates (SL1-5) were studied. All isolates had circular shape, opaque appearance and smooth surface. SL1, SL2 and SL3 were Gram-positive bacilli and endospore formers. SL4 and SL5 were Gram-negative cocci. The sensitivity test towards 17 types of antibiotics was carried out using the disc diffusion method. SL2, SL3 and SL4 were multidrug-resistant isolates. Prominently, SL4 was resistant to 10 types of antibiotics. Antibacterial activity against 11 types of bacteria was also evaluated. SL1 was found to produce antibacterial agent that inhibit the growth of Salmonella sp. and Staphylococcus epidermidis. Protease, α-amylase, lipase and another 19 types of enzyme activity were screened using enzymatic assay and API ZYM kit assay. All isolates had proteolytic and lipolytic activities. SL1, SL2 and SL5 were able to produce protease enzyme whereas SL4 was able to produce phospholipase enzyme. In API ZYM assay, all isolates were able to produce esterase (C4), esterase lipase (C8) and leucine arylamidase (Leu). However, α-amylase producer was absent. Further studies will be carried out to identify the species of all isolates.

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“…In this study, the method of isolation and identification of an unknown fungus from CSA Farm, Kanpur district using 18S rRNA gene sequence as reported in bacterial rRNA gene (Srivastava et al, 2008) was used to characterize the strains T k -5201/CSAU and T vi -4177/CSAU as members of the Trichoderma spp. The soil sample has received great attention from public due to its potential for biodiversity and biological conservation.…”

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confidence: 99%

Phylogenetic diversity analysis of Trichoderma species based on internal transcribed spacer (ITS) marker

Shahid¹,

Srivastava²,

Kumar³

et al. 2014

Afr. J. Biotechnol.

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The phylogeny of Trichoderma and the phylogenetic relationships of its species was investigated by maximum parsimony analysis and distance analysis of DNA sequences from multiple genetic loci 18S rDNA sequence analysis suggests that the genus Trichoderma evolved at the same time as Hypomyces and Fusarium and thus about 110 Myr ago 28S rDNA sequence analysis shows that the genus Trichoderma is part of a monophyletic branch within the Hypocreaceae. Most isolates of the genus Trichoderma were found to act as mycoparasites of many economically important aerial and soil-borne plant pathogens. Trichoderma has attained importance as a substitute for chemical pesticides and hence an attempt was intended to corroborate the positive relatedness of molecular and morphological characters. Two fungal strains, Trichoderma koningii T k-5201/CSAU and Trichoderma virens T vi-4177/CSAU were isolated from a soil sample collected from CSA Farm, Kanpur district of Uttar Pradesh, India. The universal primers (internal transcribed spacer, ITS) were used for the amplification of 18S rRNA gene fragment and strains were thus characterized with the help of ITS marker. It is proposed that the identified strains T. koningii T k-5201/CSAU and T. virens T vi-4177/CSAU be assigned as the type strains of a species of genus Trichoderma based on phylogenetic tree analysis together with the 18S rRNA gene sequence search in Ribosomal Database Project, small subunit rRNA and large subunit rRNA databases. The sequence was deposited in GenBank with the accession numbers KC800923 and KC800924, respectively. Thus an integrated approach of morphological and molecular markers can be employed to identify a superior strain of Trichoderma for its commercial exploitation.

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Identification of regulatory elements in 16S rRNA gene of Acinetobacter species isolated from water sample

Cited by 40 publications

References 6 publications

Molecular Detection of Gyra and Parc Genes in Multidrug Resistant Acinetobacter Baumannii Isolated From Nosocomial Infections in Children.

Molecular Detection of Gyra and Parc Genes in Multidrug Resistant Acinetobacter Baumannii Isolated From Nosocomial Infections in Children.

Biological screening of microbes isolated from soil of ex-tin mining land in Kampar Area

Phylogenetic diversity analysis of Trichoderma species based on internal transcribed spacer (ITS) marker

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