Identification of Saccharomyces cerevisiae genes leading to synthetic lethality of prion [PSI +] with SUP45 mutations

Matveenko, Andrew G.; Zemlyanko, O. M.; Zhouravleva, Galina A.

doi:10.1134/s0026893313040110

Cited by 4 publications

(4 citation statements)

References 37 publications

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“…S1C) in the [psi 2 ] strains was not affected. In contrast, overproduction of translation elongation factor eEF1A (encoded by TEF2 gene and known to increase translational suppression in general (Song et al, 1989;Matveenko et al, 2013)) increased suppression in sup45 mutants (Supporting Information Fig. S1B).…”

Section: Cur1 Enhances Phenotypic Effects Of the [Psi 1 ] Prionmentioning

confidence: 99%

To CURe or not to CURe? Differential effects of the chaperone sorting factor Cur1 on yeast prions are mediated by the chaperone Sis1

Barbitoff

Matveenko

Moskalenko

et al. 2017

Molecular Microbiology

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Yeast self-perpetuating protein aggregates (prions) provide a convenient model for studying various components of the cellular protein quality control system. Molecular chaperones and chaperone-sorting factors, such as yeast Cur1 protein, play key role in proteostasis via tight control of partitioning and recycling of misfolded proteins. In this study, we show that, despite the previously described ability of Cur1 to antagonize the yeast prion [URE3], it enhances propagation and phenotypic manifestation of another prion, [PSI ]. We demonstrate that both curing of [URE3] and enhancement of [PSI ] in the presence of excess Cur1 are counteracted by the cochaperone Hsp40-Sis1 in a dosage-dependent manner, and show that the effect of Cur1 on prions parallels effects of the attachment of nuclear localization signal to Sis1, indicating that Cur1 acts on prions via its previously reported ability to relocalize Sis1 from the cytoplasm to nucleus. This shows that the direction in which Cur1 influences a prion depends on how this specific prion responds to relocalization of Sis1.

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Section: Cur1 Enhances Phenotypic Effects Of the [Psi 1 ] Prionmentioning

confidence: 99%

To CURe or not to CURe? Differential effects of the chaperone sorting factor Cur1 on yeast prions are mediated by the chaperone Sis1

Barbitoff

Matveenko

Moskalenko

et al. 2017

Molecular Microbiology

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show abstract

“…Several attempts ta screening for such factors affecting the [PSI + ] prion or nonsense suppression have been made; however, these have yielded contradictory results. For example, SFP1 overexpression was found to enhance nonsense suppression on the [PSI + ] background, but not in other test systems [17,18,22,32]. Here, in order to uncover new mechanisms of translational accuracy, we concentrated on the effects of Sfp1 and Mcm1 on the transcription of the release factor genes.…”

Section: Discussionmentioning

confidence: 99%

“…Plasmids pRS426-SFP1 [21] and pU-MCM1 [22] were kindly provided by Tatiana M. Rogoza and Anton A. Nizhnikov, respectively. pUGC-MCM1-GFP is a pUG35-based vector obtained through a series of intermediate vectors and contains the MCM1 ORF with upstream 50 bp sequence, flanked by BglII and SacII restriction sites (the PsuI-SacII fragment of the pGPD-f1-MCM1-YFP plasmid [17]) under control of the CUP1 promoter originally derived from pRS316CG [23]. Plasmid pYX242-Nab2NLS-2mCherry [24] was a kind gift from Simon Alberti.…”

Section: Plasmidsmentioning

confidence: 99%

“…We previously investigated the possible influence of two Q/N-rich transcription factors, Mcm1 and Sfp1, on [PSI + ] properties. Both were discovered by screening using a synthetic lethality test, which helped to identify factors that affect the lethality of [PSI + ] in the presence of sup45 mutation by influencing nonsense suppression [16][17][18]. Here, we attempted to find out whether the effects of Mcm1 and Sfp1 on [PSI + ] are due to their function as transcription regulators.…”

Section: Introductionmentioning

confidence: 99%

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Transcription Factors Mcm1 and Sfp1 May Affect [PSI+] Prion Phenotype by Altering the Expression of the SUP35 Gene

Matveenko,

Mikhailichenko,

Drozdova

et al. 2024

Microbiology Research

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Mcm1 is an essential Q/N-rich transcription factor. Q/N-rich proteins interact with each other, and many affect the [PSI+] prion formed by the translation termination factor Sup35 (eRF3). We found that transient MCM1 overexpression increased nonsense suppression in [PSI+] strains and SUP35 transcription. As we had discovered similar effects of another Q/N-rich transcription factor, Sfp1, here we focus on the roles of Mcm1 and Sfp1 in SUP35 expression, as well as on the effects of Sfp1 on the expression of the gene encoding another release factor, Sup45 (eRF1). Mutations in the SUP35 promoter showed that none of the potential Mcm1 binding sites affected the Sup35 protein level or nonsense suppression, even during MCM1 overexpression. Mcm1 itself neither formed aggregates in vivo nor affected Sup35 aggregation. In contrast, a mutation in the Sfp1-binding site decreased Sup35 production and [PSI+] toxicity of excess Sfp1. Mutation of the Sfp1 binding site in the SUP45 promoter lowered SUP45 expression and increased nonsense suppression even more drastically. Our data indicate that the mechanisms of Mcm1 and Sfp1 action differ. While Mcm1 seems unlikely to directly regulate SUP35 expression, Sfp1 appears to act through its binding sites and to directly activate SUP35 expression, which in turn may influence the [PSI+] prion phenotype and toxicity.

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Identification of new genes that affect [PSI +] prion toxicity in Saccharomyces cerevisiae yeast

et al. 2016

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Identification of Saccharomyces cerevisiae genes leading to synthetic lethality of prion [PSI +] with SUP45 mutations

Cited by 4 publications

References 37 publications

To CURe or not to CURe? Differential effects of the chaperone sorting factor Cur1 on yeast prions are mediated by the chaperone Sis1

To CURe or not to CURe? Differential effects of the chaperone sorting factor Cur1 on yeast prions are mediated by the chaperone Sis1

Transcription Factors Mcm1 and Sfp1 May Affect [PSI+] Prion Phenotype by Altering the Expression of the SUP35 Gene

Identification of new genes that affect [PSI +] prion toxicity in Saccharomyces cerevisiae yeast

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