Identification of shiga toxin and intimin coding genes in Escherichia coli isolates from pigeons (Columba livia) in relation to phylotypes and antibiotic resistance patterns

Ghanbarpour, Reza; Daneshdoost, Shirin

doi:10.1007/s11250-011-0021-0

Cited by 18 publications

(11 citation statements)

References 31 publications

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“…). This lack of sensitivity for detection of stx2f can explain, at least in part, the lower prevalence of STEC strains (2·9% stx2 and 1·4% stx1 ) in pigeons of southeast Iran in comparison with our findings (Ghanbarpour and Daneshdoost ). The overall prevalence of stx2f ‐harbouring strains in pigeon populations was 18·8%, ranging from 9% to 25·8% in the four studied cities in the current study.…”

Section: Resultscontrasting

confidence: 82%

“…(), Silva et al . () and Ghanbarpour and Daneshdoost (), both known to be incapable of detecting the stx2f subtype (Ziebell et al . ; Feng et al .…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, pigeons carrying STEC can be a potential reservoir for pathogenic E. coli strains in urban and rural areas. The presence of other stx subtypes was previously reported in E. coli isolates from pigeons in Iran (Ghanbarpour and Daneshdoost ), but the occurrence of stx2f ‐harbouring E. coli has not been reported. This study aimed to investigate the prevalence, characteristics, clonal diversity and antibacterial susceptibility of stx2f + E. coli strains isolated from domestic pigeons in four provinces in Iran.…”

Section: Introductionmentioning

confidence: 85%

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Molecular characterization, genetic diversity and antibacterial susceptibility ofEscherichia coliencoding Shiga toxin 2f in domestic pigeons

Badouei

Salehi

Koochakzadeh

et al. 2014

Lett Appl Microbiol

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This study aimed to evaluate prevalence, characteristics, genotypic diversity and antibacterial susceptibility of Escherichia coli encoding Shiga toxin 2f in domestic pigeons in different provinces of Iran. A total of 117 faecal samples were collected from pigeons and were subjected to molecular detection of stx2f. In total, 20, 25·8, 21·4 and 9% of pigeons from Tehran, Ferdows, Garmsar and Babol cities carried stx2f+ isolates, respectively. Of the 460 E. coli isolates examined, 43 were stx2f+ and most also carried eae (95·3%) and astA (97·7%) genes. Some of the stx2f+ isolates harboured cnf (9·3%), but all were negative for stx1, stx2 (other subtypes) and ehly. Most Strains (90%) were assigned to B1 phylogroup and possessed Intimin-β. Fingerprinting of the stx2f+ isolates using either enterobacterial repetitive intergenic consensus sequences (ERIC) or random amplified polymorphic DNA (RAPD)-polymerase chain reaction revealed seven distinct profiles by each method, with one prevailing (65·1 and 46·5%, respectively). By the combination of methods, 10 profiles were recognized. Ten isolates from different profiles were shown to belong to O20, O78 and O115 serogroups, and eight were 100% identical in the stx2f gene sequence. The strains were consistently resistant to amoxicillin and lincospectin and commonly resistant to tetracycline (88·4%) and doxycycline (74·4%). Overall, the results indicate a limited degree of genetic diversity in stx2f-harbouring E. coli from pigeons. Significance and impact of the study: Carriage of stx2f gene tends to be underreported in pigeon Escherichia coli isolates because most routine genetic and phenotypic tests cannot efficiently target this gene or detect the toxin. Nevertheless, pigeons frequently carry E. coli strains that are stx2f-positive, and this situation is not limited to any distinct geographical area. The current results suggest that genetic background of stx2f-encoding E. coli is distinct from most Shiga toxin-producing E. coli strains. However, the factors that contribute to host preferences and pathogenicity remain unclear. These findings have public health significance that should be addressed in future research.

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Section: Resultscontrasting

confidence: 82%

“…(), Silva et al . () and Ghanbarpour and Daneshdoost (), both known to be incapable of detecting the stx2f subtype (Ziebell et al . ; Feng et al .…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 85%

See 1 more Smart Citation

Molecular characterization, genetic diversity and antibacterial susceptibility ofEscherichia coliencoding Shiga toxin 2f in domestic pigeons

Badouei

Salehi

Koochakzadeh

et al. 2014

Lett Appl Microbiol

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show abstract

“…Also, given that the area was not habited, it could have served as a safe place for other wild animals that could access the water at that point. E. coli is well-known to colonise the intestinal tract of both diseased and healthy warm-blooded animals and birds ( Barnett Foster, 2013;Ghanbarpour and Daneshdoost, 2012;Hammerum and Heuer, 2009;Oporto et al, 2008).…”

Section: North Ridingspruit Tributary (Nrt)mentioning

confidence: 99%

The impact of various land uses on the microbial and physicochemical quality of surface water bodies in developing countries: Prioritisation of water resources management areas

Hoven

Ubomba-Jaswa

Merwe

et al. 2017

Environmental Nanotechnology, Monitoring & Management

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To protect water resources, the WHO recommends assessing land use influence on water quality, taking into consideration residential development and waste disposal amongst others. Thus, we investigated the impact of unconstructed plots, an informal settlement, an urban residential area, and an industrial area on the microbiological and physicochemical quality of two main tributaries within the Klein Jukskei catchment, Johannesburg, South Africa, to identify areas where immediate resource management strategies were needed. Water samples collected from the tributaries' sources and upstream and downstream from each land use type (Winter and Spring) were analysed for E. coli (indicator organism), using the Colilert ® 18 system. Physicochemical parameters (Temperature, pH, dissolved oxygen, electrical conductivity, turbidity and total dissolved solids) were measured using multiparameter instruments. The tributaries' sources had the lowest E. coli counts (Sandspruit-0.74; North Ridingspruit-1.18 log10 MPN/100 mL) during the study. After flowing through the various land uses, mean E. coli counts reached 5.98 (Sandspruit) and 4.85 log10 MPN/100 mL (North Ridingspruit). E. coli values and all physicochemical parameters (but for pH) downstream from most of the land uses did not meet the South African drinking water quality guidelines. The informal settlement had the most negative impact on the microbial and physicochemical quality of the water within the tributaries. Thus, providing informal settlements with appropriate sanitation facilities is likely to prevent pollution of the water bodies. Protection of the sources should also be implemented 2 while industrial wastes need to be monitored for conformity with water quality guidelines before discharge.

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“…A presença de pombos em áreas urbanas tem como principais fatores favoráveis a oferta de alimento, água, abrigo e a existência de poucos predadores naturais, haja vista que esses animais apresentam baixa exigência alimentar, e que as cidades são repletas de locais propícios à reprodução da espécie, como árvores, casas, prédios e monumentos. Para Ghanbarpour & Daneshdoost (2011), esses quatro fatores, adicionados ao contato próximo com humanos e outros animais, têm contribuído com a propagação de agentes infecciosos e com a infecção humana por diversos patógenos, devido sobretudo a presença de fezes de pombos nesses espaços públicos urbanos.…”

Section: Introductionunclassified

Doenças infecciosas e parasitárias no contexto brasileiro

2021

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O Brasil, país de dimensões continentais, é reconhecido mundialmente por suas belezas naturais e seu clima tropical. É descrito na poesia cantada da música de Jorge Bem Jor como “...um país tropical, abençoado por Deus e bonito por natureza”, com todos os méritos. No entanto, esta mesma tropicalidade o torna terreno fértil para as doenças infecciosas e parasitárias, ainda mais se somarmos a isto as grandes dificuldades de educação em saúde e de infraestrutura sanitária que, em pleno século XXI, ainda são realidade no país. Neste cenário, como docente de Parasitologia e orientadora de uma liga acadêmica de Infectologia em um curso médico, costumo justificar aos meus alunos a importância do estudo das doenças infecciosas e parasitárias no contexto brasileiro parafraseando o poeta-cantor: “moramos num país tropical, abençoado por Deus e cheio de doenças infecciosas e parasitárias por natureza”. Ensino-lhes que, enquanto profissionais da saúde, devemos procurar conhecer não apenas os aspectos relacionados às manifestações clínicas, diagnóstico e tratamento destas doenças, mas também, as formas de preveni-las, se ainda não ocorreram, de controlá-las, se já estiverem ocorrendo, e, quem sabe, erradicá-las por completo de uma localidade ou população. Ao evitarmos a ocorrência de doenças infecciosas ou parasitárias, estamos contribuindo para uma melhor qualidade de vida das populações através da redução da morbimortalidade relacionada a estas doenças, o que impacta economicamente e nos sistemas de saúdes. Para tal, as medidas profiláticas devem considerar que, quanto mais informada for a população, maiores serão as chances dos indivíduos contribuírem para a efetividade das ações e, consequentemente, sua eficácia. Assim, a educação em saúde torna-se tão fundamental quanto a implementação de saneamento básico e outras medidas sanitárias para o controle da grande maioria destas doenças. Na perspectiva de proporcionar uma oportunidade para que acadêmicos, pósgraduandos e pesquisadores pudessem apresentar contribuições na temática das doenças infecciosas e parasitárias no contexto brasileiro, surgiu a ideia de produzir esta obra, a qual reúne 70 capítulos, organizados em dois volumes, sobre doenças causadas pelos mais diversos agentes infecciosos, dentre vírus, bactérias, fungos, protozoários e helmintos. Esta obra é publicada em um momento sanitário mundial gravíssimo, quando atravessamos, há mais de um ano, uma pandemia por um novo vírus descrito ao final do ano de 2019 e que, até o presente momento, já causou mais de 3 milhões de óbitos no mundo, estando o Brasil na segunda colocação em número de mortes por este vírus, ora denominado SARS-CoV-2. A doença causada pelo novo coronavírus, – a COVID-19 – trouxe-nos, além de grandes mudanças de hábitos, a certeza de que, cada vez mais é necessário aproximar a ciência das pessoas, mesmo as que não fazem parte do meio acadêmico e científico, pois só o conhecimento é capaz de ajudar a humanidade a compreender que precisamos cuidar uns dos outros e do planeta se não quisermos nos tornar mais uma espécie em extinção por ação de um agente patogênico. Que este compilado de estudos sobre doenças infecciosas e parasitárias possa ser um referencial para ampliar conhecimentos e promover discussões na temática proposta.

show abstract

Identification of shiga toxin and intimin coding genes in Escherichia coli isolates from pigeons (Columba livia) in relation to phylotypes and antibiotic resistance patterns

Cited by 18 publications

References 31 publications

Molecular characterization, genetic diversity and antibacterial susceptibility ofEscherichia coliencoding Shiga toxin 2f in domestic pigeons

Molecular characterization, genetic diversity and antibacterial susceptibility ofEscherichia coliencoding Shiga toxin 2f in domestic pigeons

The impact of various land uses on the microbial and physicochemical quality of surface water bodies in developing countries: Prioritisation of water resources management areas

Doenças infecciosas e parasitárias no contexto brasileiro

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