Identification of Specific Cell-Surface Markers of Adipose-Derived Stem Cells from Subcutaneous and Visceral Fat Depots

Abstract

SummaryAdipose-derived stem/stromal cells (ASCs) from the anatomically distinct subcutaneous and visceral depots of white adipose tissue (WAT) differ in their inherent properties. However, little is known about the molecular identity and definitive markers of ASCs from these depots. In this study, ASCs from subcutaneous fat (SC-ASCs) and visceral fat (VS-ASCs) of omental region were isolated and studied. High-content image screening of over 240 cell-surface markers identified several potential depot-specific markers of ASCs. Subsequent studies revealed consistent predominant expression of CD10 in SC-ASCs and CD200 in VS-ASCs across 12 human subjects and in mice. CD10-high-expressing cells sorted from SC-ASCs differentiated better than their CD10-low-expressing counterparts, whereas CD200-low VS-ASCs differentiated better than CD200-high VS-ASCs. The expression of CD10 and CD200 is thus depot-dependent and associates with adipogenic capacities. These markers will offer a valuable tool for tracking and screening of depot-specific stem cell populations.

Adult Stem Cell Subsets from Adult Human Dermis

“…The selection of media modifies cell morphology, population doubling-time, membrane fragility, and the expression of certain surface markers accompanying multipotent phenotypes ex vivo. Table 2 indicates the list of markers we have scrutinized on PHADs by flow cytometry: 1) the membrane metallo-endopeptidase (CD10), previously identified as a potent mesenchymal like-cell concentrator on perivascular cells [46] and fat cells derived from lipoaspirates [47], 2) the hematopoietic progenitor cell antigen CD34, widely studied due to its therapeutic potential in vasculogenesis, osteogenesis [48], and chondrogenesis [49] with debatable hepatogenic and neurogenic potential by peripheral blood and bone marrow-derived CD34-positive cells [50,51], and 3) and the Neuron-Glial 2 antigen (NG2), initially described in pericytes promoting multilineage potential [52] demonstrating an augment from 7.2%->95% for CD10, <1%-40% for CD34, and 1.2%-6% for NG2 after one week of chemically defined and serum-free media transition. The mechanism by which this condition alters the cell's epigenetic regulation of the transcription of these markers is not yet known and will be later investigated.…”

The transplantation of mesenchymal stem cells derived from unconventional sources: an innovative approach to multiple sclerosis therapy

“…AD-MSCs are characterized by positive expression of CD9, CD13, CD29, CD44, CD49e, CD54, CD59, CD73, CD90, CD105, CD106, CD146, CD166, and STRO-1 and negative expression of hematopoietic markers, such as CD11b, CD14, CD19, CD31, CD34, CD45, CD56, CD133, CD144, CD146, and HLA-DR (Zuk et al 2002;Ong et al 2014;Li et al 2015) (Table 1).…”

Adipogenic potential of stem cells derived from rabbit subcutaneous and visceral adipose tissue in vitro

“…We suspect a different way of adipogenesis activation in those cell types. Recently, Ong et al (2014) reported that high expression of CD200 in ADSC obtained from visceral fat correlates with low adipogenic capacities in humans and mice. CD200, also called OX2, is a member of the immunoglobulin superfamily of proteins, and its expression is related to various immunoregulatory activities such as cancer growth, autoimmune and allergic disorders, infection, etc.…”