Identification of Streptococcus pyogenes – Phenotypic Tests vs Molecular Assay ( spy 1258PCR): A Comparative Study

Abraham, Tintu; Sistla, Sujatha

doi:10.7860/jcdr/2016/20053.8093

Cited by 10 publications

(11 citation statements)

References 15 publications

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“…In this study, by the application of mostly applied techniques, 38 isolates out of 342 (11.1%) clinical specimens were identified as S. pyogenes. Our results showed a higher incidence of S. pyogenes compared to the level previously detected in India as 160 S. pyogenes isolates were detected in total of 34065 clinical samples (0.0047%) [9]. However, the annual incidence of S. pyogenes infections varied greatly and ranged between 0.029 and 2.84% per 100,000 population [26].…”

Section: Discussioncontrasting

confidence: 46%

“…S. pyogenes isolates can be identified according to bacitracin sensitivity [24]. In addition, other more sensitive identification methods have been introduced including GAS latex agglutination and PCR test for spy 1258 gene [9,19,25]. In this study, by the application of mostly applied techniques, 38 isolates out of 342 (11.1%) clinical specimens were identified as S. pyogenes.…”

Section: Discussionmentioning

confidence: 98%

“…e bacitracin sensitivity test was conducted according to the method of Abraham and Sistla [9]. In brief, after adjusting the optical density (OD) of the overnight culture to about 10 6 CFU/ml, it was spread by sterile swab on a blood agar plate, followed by placing a bacitracin disc in the center of each plate.…”

Section: Phenotypic Identification Of S Pyogenes Isolatesmentioning

confidence: 99%

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Prevalence and Characterization ofStreptococcus pyogenesClinical Isolates from Different Hospitals and Clinics in Mansoura

Helal

Rizk

El‐Sokkary

et al. 2020

International Journal of Microbiology

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Streptococcus pyogenes are associated with many bacterial diseases in both humans and animals and are capable of causing a multitude of human diseases. S. pyogenes isolates were identified by their bacitracin sensitivity, positive spy1258 detection, and positive GAS latex agglutination. Different isolates were typed serotypically and genotypically by BOX-PCR. Different virulence factors were identified in S. pyogenes isolates. In addition, antimicrobial resistance was tested to eleven different antibiotics. Furthermore, the resistance mechanisms were determined phenotypically by the disc diffusion method. Finally, the correlation between both molecular and serotypes identified and the profile of virulence factors and clinical and geographical sources was determined for all isolates. Thirty-eight S. pyogenes isolates were collected from different clinical sources. Resistance testing indicated high resistance to mostly used antibiotics except amoxicillin/clavulanic acid, amoxicillin, and ampicillin. Serotyping results indicated five different serotypes, M1, M2, M3, M4, and M6, in S. pyogenes isolates, while six isolates were identified as untypeable. In addition, positive PCR results identified most of the tested SAgs genes in which speJ gene was mostly identified followed by speI, speC, and ssa genes being identified in 81.6%, 63.3%, 60.5%, and 60.5%, respectively. However, speH was the least detected. In contrast, speL, speM, and smeZ genes could not be detected in all tested isolates. Finally, BOX-PCR molecular typing was a more effective clustering method when compared to the serotyping method in all S. pyogenes. In conclusion, the isolates in this study were highly resistant to mostly used antibiotics. M1 was the most identified serotype. No significant association was found between serotypes, BOX-PCR cluster groups, and SAgs genes profiles. However, by the application of BOX-PCR, effective molecular typing was obtained.

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Section: Discussioncontrasting

confidence: 46%

Section: Discussionmentioning

confidence: 98%

Section: Phenotypic Identification Of S Pyogenes Isolatesmentioning

confidence: 99%

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Prevalence and Characterization ofStreptococcus pyogenesClinical Isolates from Different Hospitals and Clinics in Mansoura

Helal

Rizk

El‐Sokkary

et al. 2020

International Journal of Microbiology

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“…The Spy 1258 is the most widely common primer used for molecular detection of S. pyogenes [20], this study detects 82.4% of bacterial isolates, in [12] agree with [10] study which also showed 100% Specifity and 87% sensitivity and [22] showed that Spy 1258 gene was specific for S. pyogenes only, but not from other species of the genus Streptococcus and common bacteria. And this result disagrees with [5] study showed all S. pyogenes isolates contain Spy 1258 genes and [21] study's conclusion to use Spy 1258 for the best results of identification.…”

Section: Discussioncontrasting

confidence: 63%

Molecular Identification of <i>Streptococcus pyogenes</i> in Isolates from Children with Pharyngitis, Gezira State, Sudan 2022

Balla¹,

Mergani²,

Medani³

et al. 2022

AiM

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Background: Streptococcus pyogenes (Group A streptococcus) is an important Gram-positive human pathogen affected the upper respiratory tract, such as the tonsils and pharynx, and is also induces post-infection diseases such as rheumatic fever and glomerulonephritis. This study aim to isolate Streptococcus pyogenes from children with pharyngitis and to evaluate the molecular identification of S. pyogenes compared with conventional methods. Methods: A cross sectional study was conducted on total of 200 throat swab samples which were collected from children with pharyngitis referred to Wad medani Pediatric Teaching Hospital and Wad medani ENT hospital from January to November 2021. Demographic and clinical data were collected by questionnaire. Throat swabs were tested with the standard microbiological techniques to isolated Group A streptococcus (GAS). Antimicrobial susceptibility testing was performed to all GAS isolates using the Kirby Bauer disk diffusion method according to clinical laboratory standard institute (CLSI) guidelines. Additionally, PCR was used to identify Spy 1258 gene of isolated bacteria. Results: From all throat swab samples screened, 51 isolates (25.5%) were identified as GAS. Antibiotic susceptibility testing revealed that all the GAS isolates were sensitive to Penicillin and Azithromycin. Sensitivity to Erythromycin, Gentamicin, Clarithromycin, Amoxicillin and Cephalexin were 88.2%, 86.3%, 45.1%, 41.2%, 13.7%, respectively. Based on PCR identification of Spy 1258 gene the percentage of isolated bacteria was 21%. Conclusion: The rate of isolated Streptococcus pyogenes was 25.

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“…S. pyogenes was identified depending on morphological properties on culture media, biochemical tests and Bacitracin susceptibility using 0.04 units Bacitracin discs (Bio analyse/ Turkey) 9,10 . S. pyogenes diagnosis was confirmed by API system (BioMerieux / France).…”

Section: Identification Of S Pyogenesmentioning

confidence: 99%

Molecular study of spy1258 and smeZ genes in Group A Streptococcal Tonsillitis

Degaim¹,

Taher²,

Shallal³

2019

J Pure Appl Microbiol

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Streptococcus pyogenes was the most common bacterial causes of tonsillitis. The detection of virulence factors of this pathogen can be used to determine pathogenic potential as a rapid screening method. A total of 109 isolates (46%) showed positive culture for S. pyogenes, these isolates recovered from 235 swabs were collected from tonsillitis patients in ENT department in Al-Habboby Teaching Hospital, Thi-Qar province, Iraq. S. pyogenes isolates exposed to detect the specific gene (spy1258)and one of the virulence factors were smeZ gene by PCR technique and DNA sequencing analysis. The PCR results recorded that 61% and 50% of isolates harbor spy1258 and smeZ genes, respectively. The sequencing of PCR products showed significant alignments identities (94-100%) to the S. pyogenes for both genes which are located in BLAST-NCBI Genbank. The four PCR products of both genes were registered in Genbank under the named as (ZKD1 spy-like gene; ZKD2 spy-like gene; ZKD3 smeZ-like gene and ZKD4 SmeZ-like gene). The results of Multiple sequence alignment analysis recorded that C > T and T > C polymorphism for smeZ gene.

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Identification of Streptococcus pyogenes – Phenotypic Tests vs Molecular Assay ( spy 1258PCR): A Comparative Study

Cited by 10 publications

References 15 publications

Prevalence and Characterization ofStreptococcus pyogenesClinical Isolates from Different Hospitals and Clinics in Mansoura

Prevalence and Characterization ofStreptococcus pyogenesClinical Isolates from Different Hospitals and Clinics in Mansoura

Molecular Identification of <i>Streptococcus pyogenes</i> in Isolates from Children with Pharyngitis, Gezira State, Sudan 2022

Molecular study of spy1258 and smeZ genes in Group A Streptococcal Tonsillitis

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Identification of Streptococcus pyogenes – Phenotypic Tests vs Molecular Assay ( spy 1258PCR): A Comparative Study

Cited by 10 publications

References 15 publications

Prevalence and Characterization ofStreptococcus pyogenesClinical Isolates from Different Hospitals and Clinics in Mansoura

Prevalence and Characterization ofStreptococcus pyogenesClinical Isolates from Different Hospitals and Clinics in Mansoura

Molecular Identification of &lt;i&gt;Streptococcus pyogenes&lt;/i&gt; in Isolates from Children with Pharyngitis, Gezira State, Sudan 2022

Molecular study of spy1258 and smeZ genes in Group A Streptococcal Tonsillitis

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Molecular Identification of <i>Streptococcus pyogenes</i> in Isolates from Children with Pharyngitis, Gezira State, Sudan 2022