Identification of suitable internal control genes for transcriptional studies in Eleusine coracana under different abiotic stress conditions

Abstract

Finger millet [ (L.) Gaertn] is an excellent food and forage crop of arid and semiarid areas in Africa and Asia. It is well adapted to drought, heat, high salinity, poor soil fertility and low pH with an efficient C carbon fixation mechanism for high yield potential. To normalize the target gene expression data, the identification of suitable reference genes is essential. Ten candidate reference genes were selected and their expression stability was analyzed in various samples treated with different abiotic stress conditions. Five different statistical algorithms: geNorm, NormFinder, BestKeeper, ΔCt, and RefFinder were used to determine the stability of these genes. Our results revealed 1 and as highly stable reference genes and2 and 4 as least stable reference genes across all the samples and suggesting that these genes could be used for accurate transcript normalization under abiotic stress. To the best of our knowledge, this is the first report on identification of suitable reference genes for accurate transcript normalization using qRT-PCR in finger millet under abiotic stress.

Identification of Reference Genes for RT-qPCR Analysis in Gleditsia microphylla under Abiotic Stress and Hormone Treatment

“…However, other researchers proved they were the best reference genes [ 7 , 40 , 41 ]. The remaining candidates showed a relatively wide range of expression profiles under the given experimental conditions, confirming again that no single gene could be used for normalization in all the samples of different tissues and under various experimental conditions, similar to the results in Osmanthus fragrans [ 42 ], Urochloa brizantha [ 43 ], Lycoris aurea [ 44 ], and Eleusine coracana [ 45 ].…”

Selection of Reference Genes for qRT-PCR in Bletilla striata under Heat Stress

“…They have been widely reported and possess good performance within a given species. For example, EF1α was the most appropriate reference gene under cold stress in Eleusine coracana (Jatav et al 2018), and under drought stress in Setaria italica (Kumar et al 2013). GAPDH was the best choice in Chinese cabbage (Qi et al 2010) under drought stress, in Eleusine coracana (Jatav et al 2018) ( Wang et al 2019).…”

“…For example, EF1α was the most appropriate reference gene under cold stress in Eleusine coracana (Jatav et al 2018), and under drought stress in Setaria italica (Kumar et al 2013). GAPDH was the best choice in Chinese cabbage (Qi et al 2010) under drought stress, in Eleusine coracana (Jatav et al 2018) ( Wang et al 2019). UBI showed peak stability under ABA stress in Hordeum brevisubulatum (Zhang et al 2018), under cold stress in Morus alba (Shukla et al 2019), under drought stress in wheat (Kiarash et al 2018;Dudziak et al 2020), and across different tissues in Miscanthus lutarioriparia (Cheng et al 2019).…”

“…It was reported that EF1α in Caragana korshinskii (Yang et al 2014) and Hordeum brevisubulatum (Zhang et al 2018), GAPDH in Eleusine coracana (Jatav et al 2018) and Caragana korshinskii (Yang et al 2014), and 18S and TUB in Panicum virgatum (Huang et al 2014) were ideal reference genes under heat stress. However, TUB has exhibited bad performance in Caragana korshinskii (Yang et al 2014).…”

Carex muskingumensis and Osmotic Stress: Identification of Reference Genes for Transcriptional Profiling by RT-qPCR

“…In PACMAD clade identification of stable RGs was reported several times in such genera as Eleusine [ 49 ], Cynodon [ 18 ] and Setaria [ 50 ].…”