Identification of suitable reference gene for quantitative transcription analysis (RT-qPCR) of Fusarium culmorum genes in infected barley plants

Faltusová, Zuzana; Pavel, Jozef; Vaculová, Kateřina; Chrpová, J.; Ovesná, Jaroslava

doi:10.1016/j.jcs.2017.12.004

Cited by 1 publication

(1 citation statement)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Also, caused by the infection, grains can be contaminated with mycotoxins produced by fungi hosting on cultural plants [ 8 ]. In cereals, Fusarium pathogenic fungi negatively affect grain production and quality due to the contamination of grain with trichotecene mycotoxins [ 9 ]. The most toxicologically important Fusarium mycotoxins are deoxynivalenol (DON), T-2 toxin (T2), HT-2 toxin (HT2), zearalenone (ZEN) and fumonisin B1 (FB1) [ 10 ] whose consumption in high doses can cause nausea, diarrhea and vomiting in humans [ 11 ], while feed refusal, also vomiting and diarrhea are common symptoms for animals [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Relationship between the Content of β-D-Glucans and Infection with Fusarium Pathogens in Oat (Avena sativa L.) Plants

Havrlentová

Gregusová

Šliková

et al. 2020

Plants

View full text Add to dashboard Cite

In human nutrition, oats (Avena sativa L.) are mainly used for their dietary fiber, β-D-glucans and protein content. The content of β-D-glucans in oat grain is 2–7% and is influenced by genetic and/or environmental factors. High levels of this cell walls polysaccharide are observed in naked grains of cultivated oat. It the work, the relationship between the content of β-D-glucans in oat grain and the infection with Fusarium graminearum (FG) and Fusarium culmorum (FC) was analyzed. The hypothesis was that oats with higher content of β-D-glucans are better protected and the manifestation of artificial inoculation with Fusarium strains is weaker. In the 22 oat samples analyzed, the content of β-D-glucans was 0.71–5.06%. In controls, the average content was 2.15% for hulled and 3.25% for naked grains of cultivated oats. After the infection, a decrease was observed in all, naked, hulled and wild oats. As an evidence of lower rate of infection, statistically significant lower percentage of pathogen DNA (0.39%) and less deoxynivalenol (DON) mycotoxin (FC infection 10.66 mg/kg and FG 4.92 mg/kg) were observed in naked grains compared to hulled where the level of pathogen DNA was 2.09% and the average DON level was 21.95 mg/kg (FC) and 5.52 mg/kg (FG).

show abstract