Identification of the class I genes of the mouse major histocompatibility complex by DNA-mediated gene transfer

Abstract

DNA-mediated gene transfer was used to identify cloned class I genes from the major histocompatibility complex of the BALB/c mouse. Three genes encoding the transplantation antigens H-2 Kd, Dd and Ld were identified as well as genes encoding the Qa-2,3 and two TL differentiation antigens. As many as 10 putative novel class I genes were detected by the association of their gene products with beta 2-microglobulin. Alloantiserum prepared to one of the novel antigens was used to demonstrate the expression of the previously undetected antigen on spleen cells of various inbred, congeneic, and recombinant congeneic strains of mice.

A Novel Receptor on Allograft (H‐2d)‐Induced Macrophage (H‐2b) toward an Allogeneic Major Histocompatibility Complex Class I Molecule, H‐2Dd, in Mice

“…The mouse MHC was named H-2 (7); and there are 5 regions commonly referred to within the H-2 complex (0.5 centimorgan), designated K, A, E, S, and D. The class I genes are contained in the K and D regions (6); and the entire constellation of alleles at individual linked regions inherited on a single chromosome is called a haplotype (e.g., K…”

“…The animals were housed in our animal facility under specific pathogen-free conditions in an air-conditioned room at 25Ϯ2 C and ഠ50% humidity. 6 cells/mouse). Seven days later the mice were sacrificed and peritoneal exudate cells (PEC) were harvested.…”

How damaged is the biologically active subpopulation of transfected DNA?

“…g(p) -p2(1 + 2q + 3q2 + * + Nqe'-) g(P) = 1 -(1 -HN)N(l + H) (11) In the limit as N becomes large, the probability of an effective pair of breaks occurring in a dimer is: g(P) = 1 -e -'(1 + H) (12) The equations for effective breakage of monomers and dimers can be combined to describe the effective breakage of partial dimers, which contain both unique and repeated segments. Let s, expressed as a fraction of 1 genome length, represent the unique portion of a partial dimer; let r, also expressed as a fraction of genome length, represent the repeated portions of a partial dimer.…”

“…Transfection of foreign DNA into somatic cells in culture has become an extremely important tool in modem molecular genetics, permitting studies of gene function (7,11,12,31,33), gene regulation (6,8,23,24,30,40,(55)(56)(57), oncogenesis (3,34,36,47,49), and somatic recombination (2, 4, 15, 17, 20, 21, 26, 29, 32, 37, 38, 41, 42, 45, 50-54, 60, 62) that were unimaginable only a few years ago. Despite the usefulness of DNA transfection, very little is known about the pathway by which the biologically active molecules travel from outside the cell into the nucleus or about the status of the DNA once it arrives (19,27).…”

Molecular biology of the mouse Q region

“…Transfection experiments with Q region genes into L cells failed in the detection of gene products on the cell surface [13,43]. The lack of cell surface expression of the polypeptides encoded by the Q genes after transfection of the genes into L cells can be explained by the early translational termina tion codon at the end of the transmembrane exon.…”