2008
DOI: 10.1002/art.24243
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Identification of the core element responsive to runt‐related transcription factor 2 in the promoter of human type x collagen gene

Abstract: Objective. Type X collagen and runt-related transcription factor 2 (RUNX-2) are known to be important for chondrocyte hypertrophy during skeletal growth and repair and development of osteoarthritis (OA) in mice. Aiming at clinical application, this study was undertaken to investigate transcriptional regulation of human type X collagen by RUNX-2 in human cells.Methods. Localization of type X collagen and RUNX-2 was determined by immunohistochemistry, and their functional interaction was examined in cultured mou… Show more

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Cited by 61 publications
(56 citation statements)
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“…1). The conserved regions contained a Runx2 binding site in the distal promoter, previously described to be functional in vivo (33,51), and a proximal Runx2 binding site, reported to be functional by in vitro assay (35). In addition, we found conserved half-sites for binding AP-1 (TGA(C/G)TCA), CREB/ATF (TGACGTCA), and C/EBP (ATTGCGCAAT) in the proximal promoter (Fig.…”
Section: Conserved Half-sites For Ap-1 Creb/atf and C/ebp Binding Isupporting
confidence: 62%
See 1 more Smart Citation
“…1). The conserved regions contained a Runx2 binding site in the distal promoter, previously described to be functional in vivo (33,51), and a proximal Runx2 binding site, reported to be functional by in vitro assay (35). In addition, we found conserved half-sites for binding AP-1 (TGA(C/G)TCA), CREB/ATF (TGACGTCA), and C/EBP (ATTGCGCAAT) in the proximal promoter (Fig.…”
Section: Conserved Half-sites For Ap-1 Creb/atf and C/ebp Binding Isupporting
confidence: 62%
“…Col10a1 gene activation requires a Runx2 binding site located in its distal promoter (33,34), as well as a non-consensus Runx2 binding site located in the proximal promoter region (35). In addition, the Fra2 knock-out phenotype is characterized by impaired hypertrophic differentiation of chondrocytes in the embryonic growth plate, along with reduced Col10a1 mRNA expression levels (36).…”
mentioning
confidence: 99%
“…2A). RUNX2 directly binds to the COL10A1 promoter and enhances its transcription (39). RUNX2 is also reported to regulate MMP13 and osteocalcin expression synergistically with C/EBP␤ (24,40).…”
Section: Discussionmentioning
confidence: 99%
“…Our analysis also revealed a Runx2-binding site (TGAGGG) and three Smad-binding elements (GTCT/AGAC) in this region (Fig. 2D) (12,22). Oligo pulldown assays were then performed to confirm specific Gli1/2 binding to this region.…”
Section: Identification Of An Ihh-responsive Element In the Col10a1 Pmentioning
confidence: 75%
“…Runtrelated transcription factor 2 (Runx2) was found to regulate expression of Col10␣1 and matrix calcification in chondrocytes (12)(13)(14)(15). In addition to Runx2, Col10␣1 expression is also acti-vated by myocyte enhancer factor-2c (Mef2c) through several conserved sequences in the promoter resembling MEF2-binding sites, which are antagonized by a co-repressor histone deacetylase 4 (Hdac4) (16,17).…”
mentioning
confidence: 99%