Identification of the flavin-dependent monooxygenase-encoding YUCCA gene family in Populus trichocarpa and their expression in vegetative tissues and in response to hormone and environmental stresses

Xia, Ye; Kang, Byung-guk; Osburn, Lori D.; Li, Yi; Cheng, Zhihui

doi:10.1007/s11240-009-9526-x

Cited by 27 publications

(23 citation statements)

References 34 publications

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“…The activity of the YUCs in auxin-induced explants cultured in vitro that was observed in the present study parallels the expectation that these genes are regulated by environmental clues, such as hormones and stress factors (Ye et al 2009). Although specific cis elements related to hormone and stress responses are present in the YUCs promoters, the gene regulation mechanisms and the operating transcription factors remain mostly undetermined (Zhao 2010).…”

Section: Discussionsupporting

confidence: 91%

LEAFY COTYLEDON2 (LEC2) promotes embryogenic induction in somatic tissues of Arabidopsis, via YUCCA-mediated auxin biosynthesis

Wójcikowska

Jaskóła

Gąsiorek

et al. 2013

Planta

141

145

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The LEAFY COTYLEDON2 (LEC2) transcription factor with a plant-specific B3 domain plays a central role in zygotic and somatic embryogenesis (SE). LEC2 overexpression induced in planta leads to spontaneous somatic embryo formation, but impairs the embryogenic response of explants cultured in vitro under auxin treatment. The auxin-related functions of LEC2 appear during SE induction, and the aim of the present study was to gain further insights into this phenomenon. To this end, the effect of LEC2 overexpression on the morphogenic responses of Arabidopsis explants cultured in vitro under different auxin treatments was evaluated. The expression profiles of the auxin biosynthesis genes were analysed in embryogenic cultures with respect to LEC2 activity. The results showed that LEC2 overexpression severely modifies the requirement of cultured explants for an exogenous auxin concentration at a level that is effective in SE induction and suggested an increase in the auxin content in 35S::LEC2-GR transgenic explants. The assumption of an LEC2 promoted increase in endogenous auxin in cultured explants was further supported by the expression profiling of the genes involved in auxin biosynthesis. The analysis indicated that YUCCAs and TAA1, working in the IPA-YUC auxin biosynthesis pathway, are associated with SE induction, and that the expression of three YUCCA genes (YUC1, YUC4 and YUC10) is associated with LEC2 activity. The results also suggest that the IAOx-mediated auxin biosynthesis pathway involving ATR1/MYB34 and CYP79B2 does not seem to be involved in SE induction. We conclude that de novo auxin production via the tryptophan-dependent IPA-YUC auxin biosynthesis pathway is implicated in SE induction, and that LEC2 plays a key role in this mechanism.Electronic supplementary materialThe online version of this article (doi:10.1007/s00425-013-1892-2) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 91%

LEAFY COTYLEDON2 (LEC2) promotes embryogenic induction in somatic tissues of Arabidopsis, via YUCCA-mediated auxin biosynthesis

Wójcikowska

Jaskóła

Gąsiorek

et al. 2013

Planta

141

145

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“…This hypothesis is supported by the evidence that IAA or pathogen infection can induce plant IAA synthesis-related genes. Exogenous IAA can induce the expression of some paralogs of the YUCCA family, which is important for plant auxin biosynthesis in Arabidopsis , in the black cottonwood Populus trichocarpa (Figure 1; Zhao et al, 2001; Xia et al, 2009). Indole-3-acetaldehyde oxidase (AAO) and nitrilase (NIT) are two protein families involved in the two Trp-dependent pathways for IAA biosynthesis in plants, respectively (Woodward and Bartel, 2005).…”

Section: Virulence Mechanism Of Pathogen-generated Iaa During Diseasementioning

confidence: 99%

Insights into Auxin Signaling in Plant?Pathogen Interactions

Fu¹,

Wang²

2011

Front. Plant Sci.

182

121

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The phytohormone auxin has been known to be a regulator of plant growth and development ever since its discovery. Recent studies on plant–pathogen interactions identify auxin as a key character in pathogenesis and plant defense. Like plants, diverse pathogens possess the capacity to synthesize indole-3-acetic acid (IAA), the major form of auxin in plants. The emerging knowledge on auxin-signaling components, auxin metabolic processes, and indole-derived phytoalexins in plant responses to pathogen invasion has provided putative mechanisms of IAA in plant susceptibility and resistance to non-gall- or tumor-inducing pathogens.

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“…Significant effort has been made to develop a transformation system for poplar species (Busov et al 2009); however, the efficient transformation system has been developed for a very limited number of poplar species/cultivars. For example, hybrid aspen clone INRA 717-IB4 (Populus tremula 9 P. alba) has been intensively used for gene transformation (Peña and Seguin 2001;Confalonieri et al 2003;Ye et al 2009). Although many other poplar species have been transformed, the transformation frequency still remains low or unstable.…”

Section: Introductionmentioning

confidence: 99%

Direct regeneration from in vitro leaf and petiole tissues of Populus tremula ‘Erecta’

Huang

Dai

2011

Plant Cell Tiss Organ Cult

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Dormant buds from a mature tree of Populus tremula 'Erecta' were incubated on a Murashige and Skoog (MS) medium supplemented with 1.0 lM thidiazuron (TDZ). Induced shoots were then proliferated on medium of MS or Woody Plant Medium (WPM), or Driver and Kuniyuki Walnut (DKW) supplemented with varying levels of benzyladenine (BA). Overall, shoots grown on MS medium supplemented with 1.25-2.5 lM BA exhibited the highest frequency of shoot proliferation ([95%) and more than 60% of responding explants produced more than five shoots per explant. Shoot organogenesis was induced from both leaf and petiole explants incubated on WPM medium containing BA, or TDZ, or zeatin. Among the different cytokinins tested, zeatin induced the highest frequency (average 72.1%) of shoot organogenesis. None of explants survived on media containing no cytokinins within 6-8 weeks following culture. Overall, a higher frequency of shoot regeneration was obtained from petioles than from leaf explants. The highest frequency of regeneration was achieved when petioles were incubated on WPM containing 10-20 lM zeatin. Addition of naphthaleneacetic acid (NAA) did not have a significant effect on shoot regeneration in all treatments. Shoot organogenesis was directly induced from petiole explants without intervening callus. Regenerated shoots were easily rooted on all tested media supplemented with 0.5 lM NAA. Rooted plants were transferred to potting mix and grown in the greenhouse.

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Identification of the flavin-dependent monooxygenase-encoding YUCCA gene family in Populus trichocarpa and their expression in vegetative tissues and in response to hormone and environmental stresses

Abstract: Auxin is a plant hormone that regulates many processes of plant growth such as apical dominance, cell growth, adventitious rooting, and fruit and seed development

Cited by 27 publications

References 34 publications

LEAFY COTYLEDON2 (LEC2) promotes embryogenic induction in somatic tissues of Arabidopsis, via YUCCA-mediated auxin biosynthesis

LEAFY COTYLEDON2 (LEC2) promotes embryogenic induction in somatic tissues of Arabidopsis, via YUCCA-mediated auxin biosynthesis

Insights into Auxin Signaling in Plant?Pathogen Interactions

Direct regeneration from in vitro leaf and petiole tissues of Populus tremula ‘Erecta’

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