Identification of the new polymorphisms in the promoter region of the CAST gene in cattle

Genetic polymorphism of two genes associated with carcass trait in Egyptian buffaloes

“…Juszczuk-Kubiak et al [24] reported the appearance of three SSCP patterns -within intron 14 of bovine CAPN1 -in 141 tested animals belonging to seven cattle breeds. These three SSCP patterns were confirmed by RFLP and gave three different genotypes, CC (35%), CT (35%) and TT (30%) due to the transition of C fi T at position 4685 in tested animals which showed T allele.…”

“…The 670-bp DNA fragment (exons 14-18) of CAPN1 gene [24] and the 400-bp DNA fragment (intron 2) of LEP gene [30] were amplified using buffalo DNA. The reaction was run in MJ research PTC-100 Thermocycler for 1 min at 94°C (denaturation), 2 min at 62°C for CAPN1 or 55°C for LEP (annealing) and 2 min at 72°C (extension) for 30 cycles.…”

“…In CAPN1 gene, more than 100 single nucleotide polymorphisms (SNPs) have been identified in cattle [46,41,24]. Among them, four polymorphisms, two non-synonymous SNPs (G316A and V530I) and two intronic SNPs (C4685T and C4751T) have been found to have significant effects on meat tenderness [41,42,52,36,44,51].…”

Determination of Calpastatin Gene Polymorphism in Kivircik Crossbred Ewes by PCR-RFLP Method

“…The calpaines play a key role in the process of meat tenderness with proteolytic activities in cold storage. CAST gene is located on the fifth chromosome of sheep and have significant effect on meat tenderness by inhibiting calpaines in post mortem process (Kawasaki and Kawashima 1996;Juszczuk-Kubiak et al 2009;Khederzadeh 2011). Previous studies have also determined the relations of CAST gene and meat quality and carcass traits in livestock animals (Morris et al 2006;Zhou and Hickford 2008;Pinto et al 2010;Li et al 2013).…”

Evaluación del efecto de los SNPs G530A (calpaína), C357G (calpastatina), G1795A (miopaladina) y G1181A (PPARGC1A) sobre la terneza de carne bovina de una región del Estado de Veracruz, México

“…Sin embargo, hay pocos estudios sobre las variaciones de secuencias o cambios en las regiones reguladoras potenciales que pudieran explicar las diferencias fenotípicas en la calidad de la carne. Juszczuk-Kubiak et al (2009), sugieren que el SNP C357G localizado en el exón 1u asociado al promotor III, podría tener influencia sobre la expresión de este gen y repercutir en rasgos importantes de la calidad como la terneza. Las múltiples funciones en los ensambles de proteínas de la línea Z y la banda I atribuidas a la proteína Miopaladina codificada por el gen MYPN, demuestran una asociación de los SNPs de este gen con la terneza.…”