Identification of the potential active components of Abelmoschus manihot in rat blood and kidney tissue by microdialysis combined with ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry

Xue, Chen; Guo, Jianming; Qian, Dawei; Duan, Jin‐Ao; Shang, Erxin; Shu, Yan; Lu, Yen‐Shen

doi:10.1016/j.jchromb.2010.12.016

Cited by 48 publications

(32 citation statements)

References 21 publications

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“…One capsule of HKC contains 0.5 g of AM. The productive process of HKC is subjected to strict quality control, and the main components are subjected to standardization (Trendafilova et al, 2011;Xue et al, 2011). In addition, HKC is not only manufactured as granules after dynamic cycle extraction and concentration by evaporating and spray drying, but also monitored for the absence of contaminants (heavy metals, pesticides, hormone and mycotoxins) prior to the formulation.…”

Section: Hkc Preparationmentioning

confidence: 99%

Huangkui capsule attenuates renal fibrosis in diabetic nephropathy rats through regulating oxidative stress and p38MAPK/Akt pathways, compared to α-lipoic acid

Mao

Shen

Wan

et al. 2015

Journal of Ethnopharmacology

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Section: Hkc Preparationmentioning

confidence: 99%

Huangkui capsule attenuates renal fibrosis in diabetic nephropathy rats through regulating oxidative stress and p38MAPK/Akt pathways, compared to α-lipoic acid

Mao

Shen

Wan

et al. 2015

Journal of Ethnopharmacology

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“…(15) Hyperoside, isoquercitrin, and quercetin monoglucuronide were also identifi ed in rat kidney blood. (54) These potentially bioactive constituents in vivo could help clarify the efficacy and safety profiles of this herb as well as potential mechanism of its effects.…”

Section: Sensitivity Analysis (Excluding Rcts In Which Patients Had Wmentioning

confidence: 99%

Efficacy and safety of Flos Abelmoschus manihot (Malvaceae) on type 2 diabetic nephropathy: A systematic review

Chen

Gong²,

Cai

et al. 2014

Chin. J. Integr. Med.

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“…32 These results showed that unbound constituents of TFA in rat blood circulation and kidney are hyperoside and isoquercetin, which might be the potential active components of TFA. Hyperoside was identified as one of the most abundant ingredients chemically characterized in TFA in a previous study.…”

mentioning

confidence: 76%

Pretreatment with the Total Flavone Glycosides of FlosAbelmoschus manihotand Hyperoside Prevents Glomerular Podocyte Apoptosis in Streptozotocin-Induced Diabetic Nephropathy

Zhou

Teng

et al. 2012

Journal of Medicinal Food

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Diabetic nephropathy (DN) is an important diabetic complication, and podocyte apoptosis plays a critical role in the development of DN. In the present study, we examined the preventive effect of the total flavone glycosides of Flos Abelmoschus manihot (TFA) on urinary microalbumin and glomerular podocyte apoptosis in experimental DN rats. The preliminary oral administration of TFA (200 mg/kg/day) for 24 weeks significantly decreased the urinary microalbumin to creatinine ratio and 24-h urinary total protein in streptozotocin-induced DN rats. Terminal deoxynucleotidyl transferasemediated dUTP nick end-labeling assay indicated glomerular cell apoptosis in DN rats was significantly improved by pretreatment with TFA. Furthermore, fluorescence-activated cell sorting and Hoechst 33342 staining suggested preincubation with hyperoside (50 and 200 lg/mL), the major active constituent of TFA, could significantly mitigate cultured podocyte apoptosis induced by the advanced glycation end-products (AGEs). Western blot analysis showed that increased caspase-3 and caspase-8 expressions induced by AGEs were also inhibited by pretreatment with hyperoside at both doses. Our results demonstrate that TFA pretreatment can decrease urinary albumin excretion in early-stage DN, which might be accomplished by preventing renal damage and podocyte apoptosis.

show abstract

Identification of the potential active components of Abelmoschus manihot in rat blood and kidney tissue by microdialysis combined with ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry

Cited by 48 publications

References 21 publications

Huangkui capsule attenuates renal fibrosis in diabetic nephropathy rats through regulating oxidative stress and p38MAPK/Akt pathways, compared to α-lipoic acid

Huangkui capsule attenuates renal fibrosis in diabetic nephropathy rats through regulating oxidative stress and p38MAPK/Akt pathways, compared to α-lipoic acid

Efficacy and safety of Flos Abelmoschus manihot (Malvaceae) on type 2 diabetic nephropathy: A systematic review

Pretreatment with the Total Flavone Glycosides of FlosAbelmoschus manihotand Hyperoside Prevents Glomerular Podocyte Apoptosis in Streptozotocin-Induced Diabetic Nephropathy

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