Identification of Three Distinct Groups of &lt;i&gt;Anopheles lindesayi&lt;/i&gt; in Japan by Morphological and Genetic Analyses

Imanishi, Nozomi; Higa, Yukiko; Teng, Hwa-Jen; Sunahara, Toshihiko; Minakawa, Noboru

doi:10.7883/yoken.jjid.2017.537

Cited by 11 publications

(2 citation statements)

References 21 publications

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“…These limitations have led to the development and incorporation of molecular tools that facilitate and speed up the identi cation of vectors in areas of high diversity. Multiplex PCR, allele-speci c PCR, and sequencing have been successfully used to diagnose Anopheles species in Malaysia [16], Germany [17], Korea [18], Romania [19], India [20], Ethiopia [21], Sri Lanka [22], Indonesia [23], Papua New Guinea [24] and several other countries [25][26][27][28][29][30]. Other authors have opted for an approach based on digestion with restriction enzymes of DNA sequences such as ITS2, D3, COI, or COII [20,[31][32][33][34][35][36][37][38]].…”

Section: Introductionmentioning

confidence: 99%

PCR-RFLP assays for the identification of Anopheles (Diptera: Culicidae) species circulating in Honduras

Escobar

Pérez

Ortiz

et al. 2022

Preprint

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Background Vector populations are a key target for malaria control and elimination. In Honduras there are at least 12 reported anopheline species, however, the definitive number of species remains uncertain. Due to the inherent limitations of morphological identification of Anophelesspecies, molecular approaches have been developed to provide accurate identification and robust surveillance of local malaria vectors. The aim of this study was to design and assess three PCR-RFLP assays to identify anopheline species in Honduras. Methods Mosquitoes captured between 2018 and 2022 in seven malaria-endemic and non-endemic departments in Honduras were analysed. The ITS2 ribosomal region and three restriction enzyme-based assays were evaluated in silico and experimentally. Results A total of 132 sequences from 12 anopheline species were analysed. The ITS2 marker showed length polymorphisms that generated products between 388 bp and 592 bp and no relevant intraspecies polymorphisms were found. Furthermore, the three PCR-RFLP assays were able to differentiate eleven species with sufficient precision and resolution. Conclusion The ITS2 region showed to be a useful molecular marker for identifying local Anophelesspecies. In addition, the PCR-RFLP assays evaluated here proved to be capable of discriminating most of the anopheline species present in Honduras. These methods provide alternatives to improve entomological surveillance of Anophelesin Honduras and other Mesoamerican countries.

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Section: Introductionmentioning

confidence: 99%

PCR-RFLP assays for the identification of Anopheles (Diptera: Culicidae) species circulating in Honduras

Escobar

Pérez

Ortiz

et al. 2022

Preprint

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“…Anopheles pleccau was able to develop sporozoites of P. falciparum , P. vivax , and P. malariae , while An. japonicus was capable of developing sporozoites of P. falciparum and P. vivax (reviewed by Imanishi et al 2018). However, the role that species of the Lindesayi Complex may play in malaria transmission is not known.…”

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confidence: 99%

A multiplex PCR assay for the identification of 5 sibling species of the Lindesayi Complex of the genus Anopheles (Diptera: Culicidae) in Bhutan

Zhou

Namgay

Saeung

et al. 2023

Journal of Medical Entomology

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Five sibling species of the Lindesayi Complex of the genus Anopheles have been discovered in Bhutan: An. druki Somboon, Namgay & Harbach, An. himalayensis Somboon, Namgay & Harbach, An. lindesayi Giles, An. lindesayi species B, and An. thimphuensis Somboon, Namgay & Harbach. The species are morphologically similar in the adult and/or immature stages. This study aimed to develop a multiplex PCR assay to identify the 5 species. Allele-specific primers were designed for specific nucleotide segments of ITS2 sequences previously reported for each species. The assay provided products of 183 bp for An. druki, 338 bp for An. himalayensis, 126 bp for An. lindesayi, 290 bp for An. lindesayi species B, and 370 bp for An. thimphuensis. The use of the assay produced consistent results. The assay is relatively inexpensive, enables the rapid identification of a large number of specimens, and will foster further studies of the Lindesayi Complex.

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Mosquito

Somboon,

Futami,

Higa

2024

Entomology Monographs

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Identification of Three Distinct Groups of <i>Anopheles lindesayi</i> in Japan by Morphological and Genetic Analyses

Cited by 11 publications

References 21 publications

PCR-RFLP assays for the identification of Anopheles (Diptera: Culicidae) species circulating in Honduras

PCR-RFLP assays for the identification of Anopheles (Diptera: Culicidae) species circulating in Honduras

A multiplex PCR assay for the identification of 5 sibling species of the Lindesayi Complex of the genus Anopheles (Diptera: Culicidae) in Bhutan

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