Identification of Three Potential circRNA Biomarkers of Polycystic Ovary Syndrome by Bioinformatics Analysis and Validation

Abstract

Objective It is well known that circRNAs are closely involved in the progression of various diseases. However, their functions and potential regulatory mechanisms in polycystic ovary syndrome (PCOS) remain largely unknown. In the present study, our aim was to investigate the potential diagnostic value of circRNAs in PCOS. Methods The circRNA dataset GSE145296, mRNA dataset GSE155489 and miRNA GSE138572 were downloaded from Gene Expression Omnibus (GEO) database. Then, differentially expressed genes (DEGs) were identified. Based on the potential interactions, a network of cirRNA-related competing endogenous RNAs (ceRNAs) was constructed. Biological functions were predicted by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses. For further validation, qRT-PCR method was used to detect the expression level of the candidate circRNAs. Then, receiver operating characteristics (ROC) were constructed to evaluate the diagnostic value of the three differentially expressed circRNA (DE-circRNA). Results We constructed a network of cirRNA-related ceRNA network. Hsa_circ_0075691, hsa_circ_0075692 and hsa_circ_0085997 were validate to be dysregulated in PCOS. Conclusion Hsa_circ_0075691, hsa_circ_0075692 and hsa_circ_0085997 may be potential diagnostic biomarkers of PCOS, but their specific regulatory mechanisms still need to be further studied.

Quantitative Proteomics Reveals That a Prognostic Signature of the Endometrium of the Polycystic Ovary Syndrome Women Based on Ferroptosis Proteins

“…PCOS patients’ impaired endometrial receptivity and reproductive outcomes may be affected by changes in numerous signal pathways as the disease progresses ( 17 , 42 , 43 ). In our study, the DEPs were shown to be involved in ferroptosis and mitochondrial dysfunction based on the KEGG pathway and subcellular location prediction system.…”

Circular RNA as a Novel Regulator and Promising Biomarker in Polycystic Ovary Syndrome

“…Furthermore, hsa_circ_0043533 and hsa_circ_0043532 also achieved high AUC values of 0.709 and 0.718, respectively [49]. Huang et al verified that three circRNAs (hsa_circ_0085997, hsa_circ_0075692, and hsa_circ_0075691) were dysregulated in PCOS and showed significant distinguishing efficiency with AUC values from 0.75 to 0.89 [104]. These three circRNAs have not been previously reported in other diseases, implying that their specificity and effectiveness make them potential biomarkers for PCOS.…”

Biomarker potential of competing endogenous RNA networks in Polycystic Ovary Syndrome (PCOS)

“…Specifically, in GCs, the downregulation of circ_0085997 leads to PRICKLE2 inhibition, whereas upregulation of miR-346 occurs. This regulatory mechanism involving the circRNA promotes the miR-346/PRICKLE2 axis, which influences the inflammatory response during the development of polycystic ovary syndrome (PCOS) [ 50 ]. MiR-346 appears to be crucial in ER-stress-induced autophagy [ 76 ], and it promotes the LIF/STAT3 signaling pathway in Hu sheep ovarian GCs [ 77 ].…”

“…Within PCOS research, ceRNETs represent a novel and promising avenue that can greatly enhance our understanding of the molecular processes driving polycystic ovarian syndrome and its pathophysiology. In this study, we conducted a comprehensive review of validated ceRNAs, including non-coding RNAs such as lncRNAs, miRNAs, and mRNAs [ 122 , 127 , 135 , 142 , 151 , 170 , 176 , 183 , 198 ] Additionally, we reviewed ceRNAs composed of circRNAs, miRNAs, and mRNAs [ 47 , 48 , 49 , 50 , 51 , 52 , 53 ]. Furthermore, we emphasized on role of ceRNAs associated with PCOS in rodent as animal models, encompassing lncRNAs, miRNAs, and mRNAs [ 204 , 207 ].…”