Identification of Two Genes in the Unique Short Region of Pseudorabies Virus; Comparison with Herpes Simplex Virus and Varicella-zoster Virus

Zijl, M van; Gulden, Hanneke van der; Wind, Niels de; Gielkens, A. L. J.; Berns, Anton

doi:10.1099/0022-1317-71-8-1747

Cited by 80 publications

(73 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The arrangement of genes in the Us region of MDV and in the alphaherpesviruses HSV-1 (McGeoch et al, 1988), VZV (Davison & Scott, 1986) and pseudorabies virus (PRV) (van Zijl et al, 1990) is shown in Fig. 3.…”

Section: Acgc T Gtggtatatgc T Caac C T Catgatagag Tacaacc Ttttccc G Amentioning

confidence: 99%

See 1 more Smart Citation

DNA sequence and organization of genes in a 5{middle dot}5 kbp EcoRI fragment mapping in the short unique segment of Marek's disease virus (strain RB1B)

Ross¹,

Binns²,

Pastorek³

1991

Journal of General Virology

View full text Add to dashboard Cite

The DNA sequence of a 5.5 kbp EcoRI fragment located in the short unique region (Us) of the 'highly oncogenic' strain RB1B of Marek's disease virus (MDV) was determined. The sequence contained six open reading frames (ORFs), four of which were homologous to proteins mapping in the Us region of herpes simplex virus type 1 (HSV-1). These include the homologues of HSV-1 protein kinase, glycoprotein D (gD), glycoprotein I (gI) and US2 which is of unknown function. The MDV ORFs had a marked bias for A or T in the third codon position and analysis of the dinucleotide frequencies showed a marginal deficit in ApG/CpT but no overall deviation of CpG from random expectations. Comparison of genes in the Us region of MDV to he~esvirus proteins confirmed and extended our previous observation that MDV is more closely related to alphaherpesviruses than to gammaherpesviruses. We also showed that MDV possessed a homologue of HSV-1 gD which is lacking in varicellazoster virus (VZV) but that MDV probably lacked homologues of US4 and US5 of HSV-1. These results show that in contrast to the genes in the long unique region which were grossly collinear in HSV, VZV and MDV, those mapping in Us show greater diversity.Marek's disease virus (MDV) DNA is a linear, 175 kbp double-stranded molecule consisting of unique long (UL) and unique short (Us) segments flanked by inverted repeats (IRs) (Cebrian et al., 1982). Recently, Buckmaster et al. (1988) identified 35 MDV genes by comparing the translated sequence of random fragments of MDV DNA with the amino acid sequences of known herpesvirus proteins. Most of the genes identified mapped in the UL region and were coUinear with genes of the alphaherpesviruses herpes simplex virus (HSV) and varicella-zoster virus (VZV). Moreover, the random sequencing study showed that MDV was more closely related to alphaherpesviruses than to beta-and gammaherpesviruses on the basis of amino acid sequence conservation. Genes of MDV that have been sequenced fully so far map in UL (Coussens & Velicer, 1988;Scott et al., 1989). In this paper we report on the DNA sequence and organization of predicted open reading frames (ORFs) in approximately half of the Us segment of MDV strain RB1B (Schat et al., 1982). We were particularly interested in determining whether MDV had a homologue of the HSV glycoprotein D (gD) and the nature and arrangement of genes adjacent to gD.Initially, random fragments of MDV DNA derived from a 23 kbp BamHI A fragment of the GA strain of MDV (Fukuchi et al., 1984) containing part of Us and most of the IRs (Fig. 1) were sequenced and the TRL

show abstract

Section: Acgc T Gtggtatatgc T Caac C T Catgatagag Tacaacc Ttttccc G Amentioning

confidence: 99%

“…Likewise, gX of PRV does not appear to have a homologue in HSV-1 but is related to HSV-2 gG (US4 of HSV-2) (McGeoch et al, 1987). MDV ORF1 is (van Zijl et al, 1990), HSV-1 and VZV (Davison & McGeoch, 1986). Arrows indicate the orientation of the encoded proteins.…”

Section: Acgc T Gtggtatatgc T Caac C T Catgatagag Tacaacc Ttttccc G Amentioning

confidence: 99%

DNA sequence and organization of genes in a 5{middle dot}5 kbp EcoRI fragment mapping in the short unique segment of Marek's disease virus (strain RB1B)

Ross¹,

Binns²,

Pastorek³

1991

Journal of General Virology

View full text Add to dashboard Cite

show abstract

“…gG is encoded by the US4 gene, which is present in most members of the subfamily Alphaherpesvirinae with the notable exception of VZV (McGeoch, 1990;Mettenleiter, 2000). PRV-gG has only limited similarity at the amino acid level with gG from HSV-2, in an 81 aa stretch, and even lower similarity with HSV-1-gG (McGeoch et al, 1987;van Zijl et al, 1990). PRV-gG is an early protein expressed as a cell-associated precursor that, following processing, is secreted and accumulates in the medium of infected cells (Bennett et al, 1986;Rea et al, 1985).…”

Section: Introductionmentioning

confidence: 99%

Glycoprotein G from pseudorabies virus binds to chemokines with high affinity and inhibits their function

Viejo‐Borbolla

Muñoz

Tabarés

et al. 2009

Journal of General Virology

View full text Add to dashboard Cite

Pseudorabies virus (PRV), also known as suid herpesvirus, is the aetiological agent of Aujeszky's disease in swine. In other animals, except higher-order primates, PRV infection is often fatal. The mechanisms of PRV pathogenesis and immune modulation are largely unknown. PRV codes for 11 glycoproteins. Among them, glycoprotein G (gG) is the most abundant PRV protein found in the supernatant of PRV-infected cell cultures. PRV-gG has low amino acid sequence similarity with gG from other animal alphaherpesviruses and its function is unknown. gG from other animal alphaherpesviruses, with the exception of at least equine herpesvirus 4, binds to chemokines. We show here that PRV-gG binds to the human chemokine CL1 and several CC and CXC human chemokines with high affinity. Chemokine-binding activity can be detected in the supernatants of PRV-infected cell cultures, and insertional inactivation of the gene encoding gG from the PRV genome results in loss of chemokine-binding activity. Binding of PRV-gG to chemokines inhibits chemokine-mediated cell migration, suggesting a role for PRV-gG in immune evasion.

show abstract

“…Cunningham et al (1992) identified the UL 13 protein as a virion component, and correlated it with a novel protein kinase activity present in nuclei of infected cells. The US3 gene has homologues in other members of the alphaherpesvirus subfamily (van Zijl et at., 1990) but not in the beta-and gammaherpesvirus lineages, whereas the UL13 gene has counterparts in alpha-, beta-and gammaherpesviruses (see McGeoch, 1989).…”

Section: Introductionmentioning

confidence: 99%

A mutant of herpes simplex virus type 1 in which the UL13 protein kinase gene is disrupted

Coulter

Moss²,

Lang³

et al. 1993

Journal of General Virology

112

117

View full text Add to dashboard Cite

Identification of Two Genes in the Unique Short Region of Pseudorabies Virus; Comparison with Herpes Simplex Virus and Varicella-zoster Virus

Cited by 80 publications

References 35 publications

DNA sequence and organization of genes in a 5{middle dot}5 kbp EcoRI fragment mapping in the short unique segment of Marek's disease virus (strain RB1B)

DNA sequence and organization of genes in a 5{middle dot}5 kbp EcoRI fragment mapping in the short unique segment of Marek's disease virus (strain RB1B)

Glycoprotein G from pseudorabies virus binds to chemokines with high affinity and inhibits their function

A mutant of herpes simplex virus type 1 in which the UL13 protein kinase gene is disrupted

Contact Info

Product

Resources

About