Identification of two mutations that cause defects in the ligninolytic system through an efficient forward genetics in the white‐rot agaricomycete <scp><i>P</i></scp><i>leurotus ostreatus</i>

Nakazawa, Takehito; Izuno, Ayako; Kodera, Rina; Miyazaki, Yasumasa; Sakamoto, Masahiro; Isagi, Yuji; Honda, Yoshio

doi:10.1111/1462-2920.13595

Cited by 30 publications

(26 citation statements)

References 44 publications

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“…According to the complete genome sequence of Pleurotus ostreatus , nine genes from the manganese peroxidase (MnP-) and VP-encoding (versatile peroxidase) gene family were identified to play significant roles in the degradation of some biopolymers like lignin (Salame et al, 2014). Investigation of the expression of certain MnP/VP genes identified the complex work for both of them in the ligninolytic system (Nakazawa et al, 2017). Additionally, mutation in chd1‐1 gene and targeted disruption of wtr1 gene leads to the ability of Pleurotus ostreatus to biodegrade wood lignin.…”

Section: Resultsmentioning

confidence: 99%

Green potential ofPleurotusspp. in biotechnology

Секан

Myronycheva

Karlsson

et al. 2019

PeerJ

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Background The genus Pleurotus is most exploitable xylotrophic fungi, with valuable biotechnological, medical, and nutritional properties. The relevant features of the representatives of this genus to provide attractive low-cost industrial tools have been reported in numerous studies to resolve the pressure of ecological issues. Additionally, a number of Pleurotus species are highly adaptive, do not require any special conditions for growth, and possess specific resistance to contaminating diseases and pests. The unique properties of Pleurotus species widely used in many environmental technologies, such as organic solid waste recycling, chemical pollutant degradation, and bioethanol production. Methodology The literature study encompasses peer-reviewed journals identified by systematic searches of electronic databases such as Google Scholar, NCBI, Springer, ResearchGate, ScienceDirect, and ISI Web of Knowledge. The search scheme was divided into several steps, as described below. Results In this review, we describe studies examining the biotechnological feasibility of Pleurotus spp. to elucidate the importance of this genus for use in green technology. Here, we review areas of application of the genus Pleurotus as a prospective biotechnological tool. Conclusion The incomplete description of some fungal biochemical pathways emphasises the future research goals for this fungal culture.

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Section: Resultsmentioning

confidence: 99%

Green potential ofPleurotusspp. in biotechnology

Секан

Myronycheva

Karlsson

et al. 2019

PeerJ

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“…The single-stranded cDNA was diluted 1/5 for real-time RT-qPCR analysis using the primer pairs (Table 5) and 2ϫ SG Fast quantitative PCR (qPCR) master mix (High Rox) (Sangon, China). The gene encoding ␤-tubulin was employed as a reference gene (61). Real-time RT-qPCR was performed in accordance with the manufacturer's instructions.…”

Section: Molecular Network Analysis (Mna)mentioning

confidence: 99%

Unusual and Highly Bioactive Sesterterpenes Synthesized by Pleurotus ostreatus during Coculture with Trametes robiniophila Murr

Shen

Zhu

et al. 2019

Appl Environ Microbiol

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Candida albicans and Cryptococcus neoformans, human-pathogenic fungi found worldwide, are receiving increasing attention due to high morbidity and mortality in immunocompromised patients. In the present work, 110 fungus pairs were constructed by coculturing 16 wood-decaying basidiomycetes, among which coculture of Trametes robiniophila Murr and Pleurotus ostreatus was found to strongly inhibit pathogenic fungi through bioactivity-guided assays. A combination of metabolomics and molecular network analysis revealed that 44 features were either newly synthesized or produced at high levels in this coculture system and that 6 of the features that belonged to a family of novel and unusual linear sesterterpenes contributed to high activity with MICs of 1 to 32 g/ml against pathogenic fungi. Furthermore, dynamic 13 C-labeling analysis revealed an association between induced features and the corresponding fungi. Unusual sesterterpenes were 13 C labeled only in P. ostreatus in a time course after stimulation by the coculture, suggesting that these sesterterpenes were synthesized by P. ostreatus instead of T. robiniophila Murr. Sesterterpene compounds 1 to 3 were renamed postrediene A to C. Real-time reverse transcription-quantitative PCR (RT-qPCR) analysis revealed that transcriptional levels of three genes encoding terpene synthase, farnesyl-diphosphate farnesyltransferase, and oxidase were found to be 8.2-fold, 88.7-fold, and 21.6-fold higher, respectively, in the coculture than in the monoculture, indicating that biosynthetic gene cluster 10 was most likely responsible for the synthesis of these sesterterpenes. A putative biosynthetic pathway of postrediene A to postrediene C was then proposed based on structures of sesterterpenes and molecular network analysis.

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“…Despite PC15 having a more complete genome than PC9, PC9 is often the preferred strain for research due to its robust growth in the laboratory. For instance, PC9 has been used to study ligninolytic activity (Nakazawa et al 2017b;Nakazawa et al 2017a;Nakazawa et al 2019), gene modification (Yoav et al 2018), and transformation (Nakazawa et al 2016). Genetic manipulation has proven to significantly increase the production of primary and secondary metabolites in several filamentous fungi that are important in industry (Banerjee et al 2003;Kück and Hoff 2010).…”

Section: Introductionmentioning

confidence: 99%

Genome sequence of the oyster mushroomPleurotus ostreatusstrain PC9

Lee

Ulzurrun

Schwarz

et al. 2020

Preprint

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The oyster mushroom Pleurotus ostreatus is a basidiomycete commonly found in the rotten wood and it is one of the most cultivated edible mushrooms globally. P. ostreatus is also a carnivorous fungus, which can paralyze and kill nematodes within minutes. However, the molecular mechanisms of the predator-prey interactions between P. ostreatus and nematodes remain unclear. PC9 and PC15 are two model strains of P. ostreatus and the genomes of both strains have been sequenced and deposited at the Joint Genome Institute (JGI). These two monokaryotic strains exhibit dramatic differences in growth, but because PC9 grows more robustly in laboratory conditions, it has become the strain of choice for many studies. Despite the fact that PC9 is the common strain for investigation, its genome is fragmentary and incomplete relative to that of PC15. To overcome this problem, we used PacBio long reads and Illumina sequencing to assemble and polish a more integrated genome for PC9. Our PC9 genome assembly, distributed across 17 scaffolds, is highly contiguous and includes six telomere-to-telomere scaffolds, dramatically improving the genome quality. We believe that our PC9 genome resource will be useful to the fungal research community investigating various aspects of P. ostreatus biology.

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Identification of two mutations that cause defects in the ligninolytic system through an efficient forward genetics in the white‐rot agaricomycete Pleurotus ostreatus

Cited by 30 publications

References 44 publications

Green potential ofPleurotusspp. in biotechnology

Green potential ofPleurotusspp. in biotechnology

Unusual and Highly Bioactive Sesterterpenes Synthesized by Pleurotus ostreatus during Coculture with Trametes robiniophila Murr

Genome sequence of the oyster mushroomPleurotus ostreatusstrain PC9

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