Identification of two novel functional p53 responsive elements in the herpes simplex virus-1 genome

Hsieh, Jui Cheng; Kuta, Ryan; Armour, Courtney R.; Boehmer, Paul E.

doi:10.1016/j.virol.2014.04.019

Cited by 6 publications

(6 citation statements)

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“…Results utilizing various p53RE‐containing luciferase reporter constructs revealed that Hr alone is unable to increase p53‐mediated transcriptional activity to any appreciable extent, but that it is capable of boosting p53‐mediated activation of the reporter construct in HEK293 and U87 cells. Interestingly, two novel types of p53RE were identified recently in the HSV‐1 genome, namely p53RE‐L and p53RE‐S [Hsieh et al, ]. Reporter cotransfection results showed that Hr can boost transcriptional activity from the novel p53RE‐S (Fig.…”

Section: Discussionmentioning

confidence: 93%

“…Having established the ability of HR to interact with both a p53RE and with p53 protein, we investigated whether HR can affect p53‐mediated target gene transcriptional activity. To perform these studies, we constructed luciferase reporter gene vectors containing the human GADD45A p53RE and also portions of the HSV‐1 genome which we and others have shown contain p53REs [Hsieh et al, ]. These reporter constructs were then cotransfected along with p53 and HR expression vectors into human embryonic kidney HEK293 cells.…”

Section: Resultsmentioning

confidence: 99%

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The Mammalian Hairless Protein as a DNA Binding Phosphoprotein

et al. 2016

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The mammalian hairless (Hr) protein plays critical roles in skin and brain tissues, but how it interacts with DNA and partner protein is only now being defined. Our initial tests of four consensus response elements, revealed that rat Hr can specifically bind to a consensus p53 response element (p53RE), 5'-AGACATGCCTAGACATGCCT-3', but not to response elements for NF-κB, TCF4 or Sp1. We then employed ChIP assays which verified that human HR binds to a p53RE of the GADD45A gene in both HEK293 (embryonic kidney) and U87 (glioblastoma) cells. Further, HR was shown to interact directly with the p53 protein in a co-immunoprecipitation assay. Cotransfections with p53RE reporter gene constructs revealed that rat Hr can boost p53-mediated transactivation of a reporter gene linked to the GADD45A p53RE, but blunts p53-mediated transactivation when the reporter gene is linked to a p21 promoter fragment containing a p53RE, with implications for the regulation of these two cell cycle control genes. Finally, our investigations of HR phosphorylation revealed that rat Hr is a substrate for PKC, but not PKA, and that human HR is phosphorylated in intact U87 cells at Ser-416, located in a highly conserved region which partially fulfills the criteria of a PKC site. We propose that mammalian Hr is a phosphoprotein which can exert cross-talk with the p53 pathway with important implications for the regulation of cell proliferation and differentiation in tissues such as skin and brain where Hr is highly expressed. J. Cell. Biochem. 118: 341-350, 2017. © 2016 Wiley Periodicals, Inc.

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Section: Discussionmentioning

confidence: 93%

Section: Resultsmentioning

confidence: 99%

The Mammalian Hairless Protein as a DNA Binding Phosphoprotein

et al. 2016

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“…p53 binds to a consensus p53 binding sequence within the non-coding region of the Human papillomavirus 77 (HPV77) genome and activates its activity in response to UV radiation ( Purdie et al, 1999 ). More recently, two novel functional p53 responsive elements (RE) have been identified in the HSV-1 genome, modulating the expression of viral proteins that may determine the progression of the lytic phase or the establishment of latency ( Hsieh et al, 2014 ). Hepatitis B virus (HBV) transcription and replication are repressed by p53 sequence-specific binding to an enhancer element within its genome.…”

Section: Discussionmentioning

confidence: 99%

Host cell p53 associates with the feline calicivirus major viral capsid protein VP1, the protease-polymerase NS6/7, and the double-stranded RNA playing a role in virus replication

Trujillo-Uscanga

Gutiérrez-Escolano

2020

Virology

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p53 is implicated in several cellular pathways such as induction of cell-cycle arrest, differentiation, senescence, and apoptosis. p53 is activated by a broad range of stress signals, including viral infections. While some viruses activate p53, others induce its inactivation, and occasionally p53 is differentially modulated during the replicative cycle. During calicivirus infections, apoptosis is required for virus exit and spread into the host; yet, the role of p53 during infection is unknown. By confocal microscopy, we found that p53 associates with FCV VP1, the protease-polymerase NS6/7, and the dsRNA. This interaction was further confirmed by proximity ligation assays, suggesting that p53 participates in the FCV replication. Knocked-down of p53 expression in CrFK cells before infection, resulted in a strong reduction of the non-structural protein levels and a decrease of the viral progeny production. These results indicate that p53 is associated with the viral replication complex and is required for an efficient FCV replication.

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“…Recently, purified natural products and herbal medicines provide a rich resource for novel antiviral drug development (OIE, 2008;Romera et al, 2014 ), such as propolis is a natural product safe for human and animal, it has been approved as health food or medicine in many countries (Baylor et al, 2002;Sforcin, 2007;Sivakumar et al, 2011). Also, green tea had virucidal effect on BHV-1 (Hsieh et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

The Effects of Green Tea and Propolis Extracts on pro-inflammatory cytokines TNF-?, IFN-?, IL2, and Immunoglobulin Production in Experimentally Infected Rabbits with Bovine Herpesvirus-1.

Zeedan¹,

El-Razik²,

Abdel-Shafy³

et al. 2019

jwpr

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Bovine herpesvirus 1 (BHV-1) is a highly contagious viral pathogen which causes infectious bovine rhinotracheitis in bovine worldwide. Currently, there is no antiviral prophylactic treatment available capable of the complete cure of the viral disease and facilitating recovery from latent infection in animals. The present study aimed to evaluate antiviral activities of Water Green Tea Extract (WGE) and Ethanol Propolis Extract (EPE) against BHV-1 virus comparing to commercial Acyclovir (ACV) in vitro in Madin-Darby Bovine Kidney (MDBK) cell line and in vivo in rabbits as a laboratory animal's model. The cytotoxicity assay was determined the safe dose of water green tea, and Ethanol propolis extracts and evaluated antiviral activity of each extract on infected MDBK with BHV-1. The fifteen rabbits were divided accidentally into five groups. Groups 1, 2 and 3 were inoculated with BHV-1 virus 10 7 TCID50/250 ul in nostrils and received propolis ethanol, water green tea extracts and ACV antiviral for 7 dpi respectively. Group 4 was inoculated with BHV-1 virus 107 TCID50/250 ul in nostrils without extracts or commercial drug. Group 5 was considered as control negative. Results of in-vitro study showed water green tea, and ethanol propolis extracts were potent inhibitor on BHV-1, which showed 80% protection against this virus and dropped in viral titer more than ACV. In vivo study of treated infected animals with WGE, EPE and ACV reduced clinical signs, elevated cytokines, and antibody production levels and failed re-isolated or detect DNA in blood or nasal samples swabs. Non treaded infected rabbits group developed respiratory clinical signs, humoral response and re-isolated BHV-1 and detected viral DNA of BHV-1 in blood, and nasal swabs from experimentally infected rabbits. In conclusion, propolis and green tea extracts were able to prevent virus replication and reduced CPE in MDBK cell cultures infected with BHV-1 and able to induce cytokines and antibodies levels production.

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Identification of two novel functional p53 responsive elements in the herpes simplex virus-1 genome

Cited by 6 publications

References 50 publications

The Mammalian Hairless Protein as a DNA Binding Phosphoprotein

The Mammalian Hairless Protein as a DNA Binding Phosphoprotein

Host cell p53 associates with the feline calicivirus major viral capsid protein VP1, the protease-polymerase NS6/7, and the double-stranded RNA playing a role in virus replication

The Effects of Green Tea and Propolis Extracts on pro-inflammatory cytokines TNF-?, IFN-?, IL2, and Immunoglobulin Production in Experimentally Infected Rabbits with Bovine Herpesvirus-1.

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