Identification of volatile active components in Acori Tatarinowii Rhizome essential oil from different regions in China by C6 glioma cells

Abstract

Background: Acori Tatarinowii Rhizome (ATR) is a well-recognized Chinese herbal medicine prescribed to treat neurological disorders. The essential oil (ATEO) is considered as the active fraction of ATR and the content of ATEO is used as the only indicator for ATR content determination. The quality of ATEO varies widely due to region difference; however, little is known about how to study ATEO quality chemically and biologically in response to region difference. Thus, it is of great importance to identify volatile active components in ATEO to conduct quality study. In this study, we analyzed ATEO from different regions in China using chemical component analysis combined with biological activity evaluation. Methods: GC-MS was used to obtain different volatile component profiles of ATEO and significantly changed volatile components were screened out. The neuroprotective activities of ATEO, including anti-oxidation, antiinflammation and neurotrophic functions, were revealed in C6 glioma cells. The correlation study between the bioactivities and the components was performed. Results: 57 volatile components, including terpenoids, phenylpropanoids, aromatic compounds, and other aliphatic compounds, were identified. 8 volatile components (β-asarone, cis-methyl isoeugenol, γ-asarone, methyleugenol, calarene, longifolene, β-caryophyllene and caryophyllene oxide) from ATEO were significantly changed due to region difference and 2 of them (β-asarone and γ-asarone) showed strong correlation with neuroprotective activities. Conclusions: Our results reveal that ATEO from different regions in China show great changes in chemical composition and biological activity. Moreover, phenylpropanoids (β-asarone and γ-asarone) present strong correlation with the bioactivities, which are considered as volatile active components in ATEO. The findings will be useful for the development of quality study of ATEO.

GC–MS Analysis of Essential Oil Extracted from Acori tatarinowii Rhizoma: An Experiment in Natural Product Analysis

“…The chemical constituents of the EOs of ATR mainly contain lignans, phenylpropanoids, and terpenoids. , Among them, asarone-based phenylpropanoids (α-asarone and β-asarone) are the characteristic constituents. As a type of solvent extraction device, a Soxhlet extractor (Figure S1) has its own advantages in EO extraction .…”

Alpha‐Asarone modulates kynurenine disposal in muscle and mediates resilience to stress‐induced depression via PGC‐1α induction

“…Acori Tatarinowii Rhizoma (Shichangpu in Chinese) is the dried rhizome of Acorus tatarinowii Schott and has been used in traditional Chinese medicine for the treatment of neurological disorders. 17 α‐Asarone is a simple phenylpropanoids in Acori Tatarinowii Rhizoma, exerting beneficial effects on alleviating neuronal excitotoxicity, anti‐inflammation and neurotransmission, and these studies are mainly focused on the regulation of central nervous system. 18 , 19 , 20 However, the role of α‐asarone in peripheral regulation against depression remains unclear.…”

Spirocyclic derivatives as antioxidants: a review

“…82 Various studies have reported its sedative, antidepressant, digestive, analgesic, diuretic, and antifungal effects. [83][84][85][86] Two new spiro alkaloids that have a naturally unusual morpholine ring in their structure, called Acortatarins A (D2) and B (D3), were isolated in 2011 from the rhizome of Acorus tatarinowii. The AO capacities of compounds D2 and D3 were determined, according to the percentage of ROS production inhibition induced by glucose in mesangial cells.…”