Identification Problems and Cryptic Diversity of Chlorella-Clade Microalgae (Chlorophyta)

Krivina, Elena S.; Темралеева, А. Д.

doi:10.1134/s0026261720060107

Cited by 20 publications

(20 citation statements)

References 19 publications

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“…2015; Song et al . 2018; Krivina & Temraleeva 2020). It is generally accepted that the best approach for creating a new Chlorella ‐clade classification is an approach that combines morphological, physiological, ecological and molecular phylogenetic methods (Hoshina et al .…”

Section: Discussionmentioning

confidence: 99%

New species Micractinium kostikovii (Chlorellaceae, Trebouxiophyceae) from Russia

Krivina

Темралеева

Sinetova

2021

Phycological Research

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SUMMARY Correctly identifying species of Chlorella‐like microalgae is difficult because of their morphological simplicity and high phenotypic plasticity. The use of molecular tools has revolutionized research on algal diversity, enabling such advances as the discovery of numerous new taxa. This article presents the results of a study of strains that are newly isolated from a freshwater Lake Prudovikov (Samara region, Russian Federation). These strains had the typical Chlorella morphology, exhibiting spherical cells and a cup‐shaped parietal chloroplast. The chloroplast contained a single pyrenoid enveloped by starch grains. However, 18S–ITS1–5.8S–ITS2 sequence analyses indicated that the studied strains are strongly allied with the well‐supported genus Micractinium. Based on the results of a comparative analysis of the morphological, molecular and environmental characteristics of the studied strains (employing a polyphasic approach), we propose that they are new species of Micractinium: Micractinium kostikovii sp. nov.

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Section: Discussionmentioning

confidence: 99%

New species Micractinium kostikovii (Chlorellaceae, Trebouxiophyceae) from Russia

Krivina

Темралеева

Sinetova

2021

Phycological Research

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“…For amplification, Screen Mix-HS mixture was used (Eurogen, Russia). Primers for PCR of the 18S and 5.8S rRNA genes and ITS1, ITS2 spacers, and amplification conditions are given in the work of Krivina and Temraleeva (2020). The detection of the target PCR products was carried out electrophoretically in a 1 % agarose gel.…”

Section: Objects Of Researchmentioning

confidence: 99%

Species delimitation and microalgal cryptic diversity analysis of the genus Micractinium (Chlorophyta)

2022

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In this article, the system of the green microalgal genus Micractinium, based on morphological, physiological, ecological and molecular data, is considered. The main diagnostic species characteristics and the taxonomic placement of some taxa are also discussed. Phylogenetic analysis showed that the genus Micractinium is characterized by high cryptic diversity. The algorithms used for species delimitation had different results on the number of potentially species-level clusters allocated. The ABGD method was less “sensitive”. The tree-based approaches GMYC and PTP showed a more feasible taxonomy of the genus Micractinium, being an effective additional tool for distinguishing species. The clustering obtained by the latter two methods is in good congruence with morphological (cell size and shape, ability to form colonies, production of bristles, chloroplast type), physiological (vitamin requirements, reaction to high and low temperatures), molecular (presence of introns, level of genetic differences, presence of CBCs or special features of the secondary structure in ITS1 and ITS2) and ecological characteristics (habitat). The polyphyly of the holotype of the genus M. pusillum as well as M. belenophorum is shown. The intron was effective as an additional tool for distinguishing species, and the results of the intron analysis should be taken into account together with other characteristics. The CBC approach, based on the search for compensatory base changes in conservative ITS2 regions, was successful only for distinguishing cryptic species from “true” members of M. pusillum. Therefore, to distinguish species, it is more effective to take into account all the CBC in ITS1 and ITS2 and analyze characteristic structural differences (molecular signatures) in the secondary structure of internal transcribed spacers. The genetic distances analysis of 18S–ITS1–5.8S–ITS2 nucleotide sequences showed that intraspecific differences in the genus ranged from 0 to 0.5 % and interspecific differences, from 0.6 to 4.7 %. Due to the polyphasic approach, it was possible to characterize 29 clusters and phylogenetic lines at the species level within the genus Micractinium and to make assumptions about the species.

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“…Microalgae are photoautotrophic microorganisms which are consisting of unicellular and multicellular groups. In general, microalgae life in waters can be in the form of colonies, filaments, and strands (single cell) [1]. Microalgae can be found in fresh, brackish and marine waters and have a role as an oxygen supply.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Culture Media Types on the Growth of Marine Microalgae Chlorella vulgaris

Mardalisa

Zalfiatri

Rahmayuni

2022

IOP Conf. Ser.: Earth Environ. Sci.

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Chlorella vulgaris is one of the marine microalgae that has potential to be developed, one of which is the renewable energy sector, biodiesel. The growth media used in the biomass production of microalgae are very various, such as Walne, Guillard, and Agriculture Fertilizer (Urea). This study aims to determine the effect of types of growth media on population density, absolute growth, relative growth rate, and specific growth rate of C. vulgaris. Study on 3 media types of microalgae growth shows the density of C. vulgaris cultured on a laboratory scale experienced the highest peak density in the Guillard media on the 3rd day around 7430 x 104 cells/mL. However, the lowest density peak was in the Urea culture about 2520 x 104 cells/mL, followed by Walne media around 3335 x 104 cells/mL. The highest absolute growth average was found in the Guillard media about 6311 x 104 cells/mL and the lowest was in the Urea and Walne media about 1401 x 104 cells/mL and 2216 x 104 cells/mL, respectively. Meanwhile, in C. vulgaris culture, it was found that Guillard and Walne media produced the highest relative growth of 564% and 198% and the lowest was at 125% for Agriculture Fertilizer (Urea). In the process of culturing C. vulgaris for 6 days, it was found that Guillard media showed the highest average specific growth rate of 58% per day and the lowest was on Urea culture as much as 27% per day, followed by Walne media about 36%.

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Identification Problems and Cryptic Diversity of Chlorella-Clade Microalgae (Chlorophyta)

Cited by 20 publications

References 19 publications

New species Micractinium kostikovii (Chlorellaceae, Trebouxiophyceae) from Russia

New species Micractinium kostikovii (Chlorellaceae, Trebouxiophyceae) from Russia

Species delimitation and microalgal cryptic diversity analysis of the genus Micractinium (Chlorophyta)

The Effect of Culture Media Types on the Growth of Marine Microalgae Chlorella vulgaris

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