Identification, quantification and antioxidant activity of acylated flavonol glycosides from sea buckthorn (Hippophae rhamnoides ssp. sinensis)

Method validation of analytical method for 12 flavonol glycosides in foods using ultra high-performance liquid chromatography coupled with photodiode array detection

“…All the values for the validation of this study were in accordance with the FDA guidelines for bioanalytical validation (26). Moreover, previous research (19) reported that the recovery values for flavonol glycosides in sea buckthorn were in the range of 94.5-106.2%, similar to the results obtained from recovery values in this study using UHPLC-PDA detection coupled with a liquid solvent extraction method. The results of the precision and accuracy analysis are shown in Table 2 -4. Consequently, the UHPLC method suggested in this study could be more conveniently applied for the analyses of 12 kinds of flavonol glycosides in buckwheat, blacktea, and wildparsley using UHPLC/PDA coupled with a simple liquid extraction method.…”

“…The most general method for the identification and quantification of flavonol glycosides involves a high-performance liquid chromatography (HPLC) system coupled with MS/MS or hybrid mass spectrometry (11)(12)(13) and single mass spectrometry (15)(16)(17). The investigation reports that formation of flavonol glycosides using nuclear magnetic resonance (NMR) (17)(18)(19) has also become a common research topic in recent years. However, the analytical methods using LC/MS, LC/MS/MS, or NMR have not been easily available in some laboratories because of their high cost.…”

Metabolic discrimination of sea buckthorn from different Hippophaë species by 1H NMR based metabolomics

“…Thus, some of the Hippophaë species can be visually identified. By comparing the 1 H NMR spectra of the standard compounds, adding relevant reference compounds directly to a pH-adjusted NMR sample, and comparisons to the published literature 8, 14, 20, 33–36 , a total of 36 metabolites were finally identified. The detailed information for the assigned peaks can be found in Table 1 and Supplementary Fig.…”

“…The standard compounds were purchased from Ruifensi Biological Technology Co., Ltd. (Chengdu, China), Maisite Biological Technology Co., Ltd. (Chengdu, China) and Pusi Biological Technology Co., Ltd. (Chengdu, China). The detailed information were shown in Supplementary Table S2, and the structures of all standard compounds were unambiguously identified based on their 1 H, 13 C NMR spectral data, LC-MS data, and published literature 33–35, 50 .…”

“…The NMR signals were assigned and identified with the aid of published literature 14, 33–35, 51 and the Spectral Database for Organic Compounds (http://sdbs.db.aist.go.jp). Additionally, the identifications were verified by carefully comparing the 1 H NMR spectra of the standard compounds, and adding relevant standard compounds directly to pH-adjusted NMR samples 14, 27 .…”