Identification, sequence, and expression of caveolin-2 defines a caveolin gene family.

Scherer, Philipp E.; Okamoto, Takashi; Chun, Miyoung; Nishimoto, Ikuo; Lodish, Harvey F.; Lisanti, Michael P.

doi:10.1073/pnas.93.1.131

Cited by 519 publications

(478 citation statements)

References 31 publications

Supporting

Mentioning

462

Contrasting

Unclassified

Order By: Relevance

“…Interestingly, among the fragments isolated, a 274 bp fragment resulted similar to mouse caveolin-1 cDNA. Analysis with BLAST program revealed that the PCR fragment isolated was 99% identical over 274 bases to the 3 0 end of the mouse caveolin-1 isoform a mRNA (Figure 1a) (Kogo and Fujimoto, 2000) and 89% identical over 92 bases to the human caveolin-1 mRNA (Glenney, 1992), but was not similar to the mouse or human caveolin-2 or caveolin-3 genes (Scherer et al, 1996;Way and Parton, 1996) (data not shown). PCR product corresponding to caveolin-1 was labeled and used as probe for Northern blot analysis to screen an extended panel of C4HD MPA-treated and untreated tumors from four different animals ( Figure 1b).…”

Section: Resultsmentioning

confidence: 99%

Progestin-induced caveolin-1 expression mediates breast cancer cell proliferation

et al. 2006

View full text Add to dashboard Cite

Progestin regulation of gene expression was assessed in the progestin-dependent murine tumor line C4HD which requires MPA, a synthetic progestin, for in vivo growth and expresses high levels of progesterone receptor (PR). By using suppressive subtractive hybridization, caveolin-1 was identified as a gene whose expression was increased with in vivo MPA treatment. By Northern and Western blot analysis, we further confirmed that caveolin-1 mRNA and protein expression increased in MPA-treated tumors as compared with untreated tumors. When primary cultures of C4HD cells were treated in vitro with MPA, caveolin-1 levels also increased, effect that was abolished by pre-treatment with progestin antagonist RU486. In addition, MPA promoted strong caveolin-1 promoter transcriptional activation both in mouse and human breast cancer cells. We also showed that MPA regulation of caveolin-1 expression involved in activation of two signaling pathways: MAPK and PI-3K. Short-term MPA treatment of C4HD cells led to tyrosine phosphorylation of caveolin-1 protein, where Src was the kinase involved. Additionally, we showed that MPA-induced association of caveolin-1 and PR, which was detected by coimmunoprecipitation and by confocal microscopy. Finally, we proved that MPA-induced proliferation of C4HD cells was inhibited by suppression of caveolin-1 expression with antisense oligodeoxynucleotides to caveolin-1 mRNA. Furthermore, we observed that inhibition of caveolin-1 expression abrogated PR capacity to induced luciferase activity from a progesterone response elementdriven reporter plasmid. Comprehensively, our results demonstrated for the first time that caveolin-1 expression is upregulated by progestin in breast cancer. We also demonstrated that caveolin-1 is a downstream effector of MPA that is partially responsible for the stimulation of growth of breast cancer cells.

show abstract

Section: Resultsmentioning

confidence: 99%

Progestin-induced caveolin-1 expression mediates breast cancer cell proliferation

et al. 2006

View full text Add to dashboard Cite

show abstract

“…Caveolae were first recognized in epithelial cells by electron micrograph techniques over 50 years ago [25]. There are three known caveolin proteins, caveolin 1 (CAV1, with splice variants α and β), caveolin 2 (CAV2), and caveolin 3 (CAV3) [26][27][28]. CAV1 and CAV2 have overlapping expression patterns in a variety of cell types including, neurons and glia [29,30], endothelial [31], and epithelial cells [32].…”

Section: Caveolins: Important For the Trafficking And Clustering Of Mmentioning

confidence: 99%

Caveolin proteins and estrogen signaling in the brain

Luoma

Boulware

Mermelstein

2008

Molecular and Cellular Endocrinology

View full text Add to dashboard Cite

Best described outside the nervous system, caveolins are structural proteins that form caveolae, functional microdomains at the plasma membrane that cluster related signaling molecules. Caveolin associated proteins include G protein coupled receptors and G proteins, receptor tyrosine kinases, as well as protein kinases, ion channels and various other signaling enzymes. Not surprisingly, a wide array of biological disorders are thought to be rooted in caveolin dysfunction. In addition, caveolins also traffic and cluster estrogen receptors to caveolae. Interactions between the estrogen receptors ERα and ERβ with caveolins appear critical in many non-neuronal cell types, e.g. disruption of normal function may underlie many forms of breast cancer. Recent findings suggest caveolins may also play an essential role in membrane estrogen receptor function in the nervous system. Not only are they expressed in neurons and glia, but different caveolin isoforms also appear necessary to generate distinct functional signaling complexes. With membrane estrogen receptors responsible for the efficient activation of a multitude of intracellular signaling pathways, which in turn influence a wide variety of nervous system functions, caveolin proteins are poised to act as the central coordinators of these processes.

show abstract

“…Recently two homologues of the protein have been identified [29][30][31], with one of these homologues being expressed exclusively in muscle [29,31]. In this review for the sake of simplicity, caveolin-1 will be used instead of VIP21-caveolin (see [41]).…”

Section: Caveolae and Vip21-caveolinmentioning

confidence: 99%

And still they are moving… Dynamic properties of caveolae

Kurzchalia

Parton

1996

FEBS Letters

View full text Add to dashboard Cite

Caveolae are structures found on the surface of many mammalian cells. In the last few years the biogenesis and the function of these organelles have been intensively investigated but many challenging questions remain. One of these is whether caveolae are statically attached to the cytoplasmic surface of the plasma membrane or are moving to other intracellular organelles. Also the cycling of the caveolar coat component, VIP21-caveolin, is a subject of intensive discussion. The solution to these problems could give an insight into the understanding of caveolar function.

show abstract

Identification, sequence, and expression of caveolin-2 defines a caveolin gene family.

Cited by 519 publications

References 31 publications

Progestin-induced caveolin-1 expression mediates breast cancer cell proliferation

Progestin-induced caveolin-1 expression mediates breast cancer cell proliferation

Caveolin proteins and estrogen signaling in the brain

And still they are moving… Dynamic properties of caveolae

Contact Info

Product

Resources

About