Identifying exposition to low oxygen environment in human macrophages using secondary ion mass spectrometry and multivariate analysis

Abstract

Our study demonstrates that macrophage molecular signature to low oxygen environment is dependent on their polarization. TOF-SIMS shows the clear capability to produce species revealing this exposition. This result opens the way to the use of TOF-SIMS as a tool to explore hypoxia in human tissues.

Chemical Analysis of Single Cells

“…Meanwhile, the chemical mapping of single cell components showed that cholesterol and DAG tend to migrate to the surrounding of the cells after high-dose Ag NPs treatment. Another application of TOF-SIMS (with a 25 keV Bi 3+ primary ion beam) was performed by Court et al to describe molecular changes of how various polarizations (expression of different functional programs in response to microenvironmental signals) are modified by low-oxygen exposure of macrophage cells . Macrophages are innate immune cells presenting a strong phenotypic plasticity and are deeply involved in tissue homeostasis.…”

“…Another application of TOF-SIMS (with a 25 keV Bi 3+ primary ion beam) was performed by Court cells. 164 Macrophages are innate immune cells presenting a strong phenotypic plasticity and are deeply involved in tissue homeostasis. After staining with phalloidin, the morphology of macrophages with various polarizations was studied using confocal microscopy.…”