Identifying for some Immune and Hematological Indicators in Crohn,s Patients Infested through Entamoeba histolytica Infection

Abstract

Objective: The current study aimed to diagnose the Entamoeba histolytica parasite in crohn,s disease patients . addition to measure immunological indicators (interleukin-34 and interleukin-38) and some hematological indicators. Methods: 150 samples of blood and stool were collected from the people participating in the study, which included 75 patients with crohn,s disease and 75 controls attended to Al-sader teaching hospital and Al-hakeem hospital in Province of Najaf from September 2020 to December 2020, and microscopic examination was performed for all stool samples and ABO blood group test also performed for all blood samples in addition to measure concentration levels of IL-34 and IL-38 by ELISA system from blood. Results: the results of microscopic examination revealed of 14 of total 75 patients with crohn,s disease were infected with Entamoeba histolytica while there were 5 participatants infected with Entamoeba histolytica from total 75 control group. Whereas the ABO blood group test revealed that highest rate of the patients suffering from crohn,s disease were from blood group B (40%), while the ABO blood group test for control group revealed that most of the people who participated were from blood group A (42.6%). The results of ELISA test revealed that the IL-34 and IL-38 is highest in patients group (627.0± 117.147) , (522.9 ± 99.922) mor than control group (203.6 ± 157.351) , (153.0 ± 109.553) respectively with significant p value (<0.001).