Identifying host microRNAs in bronchoalveolar lavage samples from lung transplant recipients infected with Aspergillus

Gohir, Wajiha; Klement, William; Singer, L.G.; Palmer, Scott M.; Mazzulli, Tony; Keshavjee, S.; Husain, Shahid

doi:10.1016/j.healun.2020.07.014

Cited by 11 publications

(4 citation statements)

References 36 publications

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“…Also, the antigen 85 complex (Ag85) containing Ag85A, Ag85B, Ag85C, which are involved in the mycobacterial pathogenesis and cell wall synthesis (Wiker & Harboe, 1992 ) are found in exosome‐derived M. tuberculosis ‐infected macrophages, BALF of female BALB/c mice and circulation of TB patients (Han et al, 2012 ; Shende et al, 2007 ). Furthermore, miR‐155, miR‐26a, mir‐21 miR‐29a miR‐424, considered as promising circulating miRNA of TB infection and therapy are also existing in BAL (Bibaki et al, 2018 ; Dyskova et al, 2015 ; Gohir et al, 2020 ; Kim et al, 2018 ; Kishore et al, 2018 ; Sinigaglia et al, 2020 ). These mycobacterial antigens and miRNA would further be identified as the biomarkers in the diagnosis of TB through BALF.…”

Section: Exosomes In Infective Diseasesmentioning

confidence: 99%

The role of exosomes from BALF in lung disease

Liu

Yan

Tong

et al. 2021

Journal Cellular Physiology

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Exosomes are released from a variety of immune cells and nonimmune cells, the phospholipid vesicle bilayer membrane structure actively secreted into tissues. Recently, exosomes were demonstrated to be effectively delivered proteins, cholesterol, lipids, and amounts of DNA, mRNA, and noncoding RNAs to a target cell or tissue from a host cell. These can be detected in blood, urine, exhaled breath condensates, bronchoalveolar lavage fluid (BALF), ascites, and cerebrospinal fluid. BALF is a clinical examination method for obtaining alveolar cells and biochemical components, reflecting changes in the lungs, so it is also called liquid biopsy. Exosomes from BALF become a new method for intercellular communication and welldocumented in various pulmonary diseases. In chronic obstructive pulmonary disease (COPD), BALF exosomes can predict the degree of COPD damage and serve as an effective monitoring indicator for airflow limitation and airway remodeling. It also mediates antigen presentation in the airways to the adaptive immune system as well as costimulatory effects. Furthermore, BALF exosomes from acute lung injury and infective diseases are closely related to various infections and lack of oxygen status.BALF exosomes play an important role in the diagnosis and prognosis of lung cancer.The effect of immunomodulatory role for BALF exosomes in adaptive and innate immune responses has been studied in sarcoidosis. The intercellular communication in the microenvironment of BALF exosomes in pulmonary fibrosis and lung remodeling have been studied. In this review, we summarize the novel findings of exosomes in BALF, executed function by protein, miRNA, DNA cytokine, and so on in several pulmonary diseases.

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Section: Exosomes In Infective Diseasesmentioning

confidence: 99%

The role of exosomes from BALF in lung disease

Liu

Yan

Tong

et al. 2021

Journal Cellular Physiology

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“…In our study miR-7975 exhibited high fold changes, respectively, 8.6 and 6.7 in FS and in FP lesions. Previous studies have shown that miR-7975 is involved in regulation of lung inflammation and in lung cancer 33 , 34 . In addition, a previous study indicated that miR-5001 expression was low in colorectal tumors and downregulation of miR-5001 that targets HES6 gene promoted cell proliferation 35 .…”

Section: Discussionmentioning

confidence: 99%

Potential miRNA biomarkers and therapeutic targets for early atherosclerotic lesions

Karere

Glenn

et al. 2023

Sci Rep

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Identification of potential therapeutic targets and biomarkers indicative of burden of early atherosclerosis that occur prior to advancement to life-threatening unstable plaques is the key to eradication of CAD prevalence and incidences. We challenged 16 baboons with a high cholesterol, high fat diet for 2 years and evaluated early-stage atherosclerotic lesions (fatty streaks, FS, and fibrous plaques, FP) in formalin-fixed common iliac arteries (CIA). We used small RNA sequencing to identify expressed miRNAs in CIA and in baseline blood samples of the same animals. We found 412 expressed miRNAs in CIA and 356 in blood samples. Eight miRNAs (miR-7975, -486-5p, -451a, -191-5p, -148a-3p, -17-5p, -378c, and -144-3p) were differentially expressed between paired fatty streak lesion and no-lesion sites of the tissue, and 27 miRNAs (e.g., miR-92a-3p, -5001, -342-3p, miR-28-3p, -21-5p, -221-3p, 146a-5p, and -16-5p) in fibrous plaques. The expression of 14 blood miRNAs significantly correlated with extent of lesions and the number of plaques. We identified coordinately regulated miRNA-gene networks in which miR-17-5p and miR-146a-5p are central hubs and miR-5001 and miR-7975 are potentially novel miRNAs associated with early atherosclerosis. In summary, we have identified miRNAs expressed in lesions and in blood that correlate with lesion burden and are potential therapeutic targets and biomarkers. These findings are a first step in elucidating miRNA regulated molecular mechanisms that underlie early atherosclerosis in a baboon model, enabling translation of our findings to humans.

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“…Apoptotic signaling in cancer [265] downregulated: blood plasma-derived EVs [145] miR-7975 MTDH Possible involvement in atherosclerosis [266], in lung inflammation, and cancer [267,268] upregulated: serum of sALS patients [155] miR-7977 CD84, MRPS12, MRPL27, MUC19, TRAPPC2, SIRT3, Hyppo-YAP signaling…”

Section: Stat3 Mmp2 Egfr/akt2/ccnd1 Signalingmentioning

confidence: 99%

Recent Advances in Extracellular Vesicles in Amyotrophic Lateral Sclerosis and Emergent Perspectives

Afonso

Cavaleiro

Valero

et al. 2023

Cells

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Amyotrophic lateral sclerosis (ALS) is a severe and incurable neurodegenerative disease characterized by the progressive death of motor neurons, leading to paralysis and death. It is a rare disease characterized by high patient-to-patient heterogeneity, which makes its study arduous and complex. Extracellular vesicles (EVs) have emerged as important players in the development of ALS. Thus, ALS phenotype-expressing cells can spread their abnormal bioactive cargo through the secretion of EVs, even in distant tissues. Importantly, owing to their nature and composition, EVs’ formation and cargo can be exploited for better comprehension of this elusive disease and identification of novel biomarkers, as well as for potential therapeutic applications, such as those based on stem cell-derived exosomes. This review highlights recent advances in the identification of the role of EVs in ALS etiopathology and how EVs can be promising new therapeutic strategies.

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Identifying host microRNAs in bronchoalveolar lavage samples from lung transplant recipients infected with Aspergillus

Cited by 11 publications

References 36 publications

The role of exosomes from BALF in lung disease

The role of exosomes from BALF in lung disease

Potential miRNA biomarkers and therapeutic targets for early atherosclerotic lesions

Recent Advances in Extracellular Vesicles in Amyotrophic Lateral Sclerosis and Emergent Perspectives

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