Identifying potential cross‐talk signatures for the occurrence of atherosclerosis in diabetic erectile dysfunction

Yuan, Peipei; Sun, Taotao; Han, Zhengyang; Chen, Yinwei

doi:10.1111/andr.13366

Cited by 2 publications

(1 citation statement)

References 63 publications

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“…These cytokines are involved in the regulation of endothelial and muscle function and play an important role in the remodeling and repair of cavernous tissues. 12 13 14 15 Moreover, the growth pattern of CC FBs means that these cells are suited to fulfilling a scaffold function. 16 17 When we resuspended CC FB cells in Matrigel for 3D culture, they formed a network-like scaffold structure independently, which is the basis for forming the spongy, porous structure of CC tissue ( Figure 3j ).…”

Section: Resultsmentioning

confidence: 99%

Artificial cavernosa-like tissue based on multibubble Matrigel and a human corpus cavernous fibroblast scaffold

Chen,

Zhou,

Yan

et al. 2024

Asian Journal of Andrology

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Ex vivo tissue culture of the human corpus cavernosum (CC) can be used to explore the tissue structural changes and complex signaling networks. At present, artificial CC-like tissues based on acellular or three-dimensional (3D)-printed scaffolds are used to solve the scarcity of primary penis tissue samples. However, inconvenience and high costs limit the wide application of such methods. Here, we describe a simple, fast, and economical method of constructing artificial CC-like tissue. Human CC fibroblasts (FBs), endothelial cells (ECs), and smooth muscle cells (SMCs) were expanded in vitro and mixed with Matrigel in specific proportions. A large number of bubbles were formed in the mixture by vortexing combined with pipette blowing, creating a porous, spongy, and spatial structure. The CC FBs produced a variety of signaling factors, showed multidirectional differentiation potential, and grew in a 3D grid in Matrigel, which is necessary for CC-like tissue to maintain a porous structure as a cell scaffold. Within the CC-like tissue, ECs covered the surface of the lumen, and SMCs were located inside the trabeculae, similar to the structure of the primary CC. Various cell components remained stable for 3 days in vitro, but the EC content decreased on the 7th day. Wingless/integrated (WNT) signaling activation led to lumen atrophy and increased tissue fibrosis in CC-like tissue, inducing the same changes in characteristics as in the primary CC. This study describes a preparation method for human artificial CC-like tissue that may provide an improved experimental platform for exploring the function and structure of the CC and conducting drug screening for erectile dysfunction therapy.

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Section: Resultsmentioning

confidence: 99%

Artificial cavernosa-like tissue based on multibubble Matrigel and a human corpus cavernous fibroblast scaffold

Chen,

Zhou,

Yan

et al. 2024

Asian Journal of Andrology

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Association of erectile dysfunction and peripheral arterial disease in NHANES 2001-2004: a cross-sectional study

Wang,

2024

Front. Endocrinol.

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ObjectiveTo evaluate the association between Erectile dysfunction (ED) and peripheral arterial disease (PAD) in adult American males using a large database.MethodsThe relationship between ED and PAD prevalence among participants in the 2001-2004 National Health and Nutrition Examination Survey (NHANES) database was assessed using a series of statistical analyses. ED was evaluated based on a single-item measure of self-reported erection problems from the Massachusetts Male Aging Study. PAD was defined as ankle-brachial index (ABI) < 0.9 in at least one leg. Multifactorial logistic regression models were used to investigate the association between ED and PAD.ResultsA total of 2394 participants were enrolled, of whom 905 individuals (37.8%) were diagnosed with ED. After adjusting for confounding variables, the association between ED and PAD remained positive, with an odds ratio of 2.05 (95% confidence interval 1.24-3.39). Subgroup analysis revealed that the relationship between ED and PAD was significant in patients aged >50 years old, without hypertension, without diabetes, without cardiovascular disease, without high cholesterol, former smokers, low physical activity levels, and a body mass index of 25-30 (P < 0.05). In addition, all subgroups analyzed were evaluated for any potential interaction, and no statistically significant association was discovered.ConclusionsIn a sample of US adults aged ≥40, this cross-sectional study found that ED is related to a higher occurrence of PAD. ED may be an independent predictor of PAD, and thus it should be considered in the treatment of patients with ED.

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Identifying potential cross‐talk signatures for the occurrence of atherosclerosis in diabetic erectile dysfunction

Cited by 2 publications

References 63 publications

Artificial cavernosa-like tissue based on multibubble Matrigel and a human corpus cavernous fibroblast scaffold

Artificial cavernosa-like tissue based on multibubble Matrigel and a human corpus cavernous fibroblast scaffold

Association of erectile dysfunction and peripheral arterial disease in NHANES 2001-2004: a cross-sectional study

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