Identifying potential ligands specifically binding to beta1-adrenoceptor from Radix Aconiti Lateralis Praeparata extract by affinity chromatographic method

Jin, Yahui; Chen, Yuanyuan; Jiao, Meizhi; Liang, Qi; Zhang, Guodong; Quan, Jia; Zhao, Xinfeng

doi:10.1016/j.jpba.2022.115022

Cited by 5 publications

(1 citation statement)

References 44 publications

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“…Several studies have demonstrated that alkaloids found in Fuzi have significant anti‐tumor effects (Dai et al, 2023; Fan et al, 2016; Fu et al, 2022; Li et al, 2022; Yu et al, 2021; Zhou et al, 2015). These alkaloids can be divided into three general categories based on structure: diester‐type alkaloids, such as aconitine, mesaconitine, and hypaconitine; monoester‐type alkaloids, such as benzoyl aconitine, benzoyl mesaconitine, and benzoyl hypaconitine; and alcohol amine‐type alkaloids, such as aconine, mesaconine, and hypaconine, among which diester‐type alkaloids are considered the active and toxic components of Fuzi (Gao et al, 2022; Jang et al, 2022; Jiang et al, 2022; Jin et al, 2022; Punia et al, 2022; Tian et al, 2022; Wang et al, 2022; Xin et al, 2022; Yang et al, 2022). The most abundant alkaloids found in Fuzi are crassicauline A, fuziline, karacoline, and songorine.…”

Section: Introductionmentioning

confidence: 99%

Development of an ultra‐high‐performance liquid chromatography–tandem mass spectrometry method for the simultaneous determination of crassicauline A, fuziline, karacoline, and songorine in rat plasma and application in their pharmacokinetics

Chen,

Wang,

Luo

et al. 2024

Biomedical Chromatography

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In this paper, an ultra‐high‐performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was developed for quantifying the levels of crassicauline A, fuziline, karacoline, and songorine in rat plasma. After processing the rat plasma, the proteins in the plasma were separated by extracting the analytes with acetonitrile–methanol (9:1, v/v). The chromatographic column used was the UPLC HSS T3 column, and the mobile phase (methanol–water with 0.1% formic acid) under a gradient elution profile was used to separate the four compounds, with elution times for each analyte being less than 5 min. Electrospray ionization in positive‐ion mode and operating in multiple reaction monitoring mode was used for quantitative analysis. Crassicauline A, fuziline, karacoline, and songorine were administered to 48 rats (n = 6 per group) orally (5 mg/kg) and intravenously (0.5 mg/kg). The standard curves demonstrated excellent linearity in the range of 1–2500 ng/mL, wherein all r values were greater than 0.99. The UPLC–MS/MS method for the determination of crassicauline A, fuziline, karacoline, and songorine in rat plasma was successfully applied in determining their pharmacokinetics parameters, from which their oral bioavailabilities were calculated to be 18.7%, 4.3%, 6.0%, and 8.4%, respectively.

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