Identifying repeating motifs in the activation of synchronized bursts in cultured neuronal networks

Raichman, Nadav; Ben‐Jacob, Eshel

doi:10.1016/j.jneumeth.2007.12.020

Cited by 91 publications

(118 citation statements)

References 49 publications

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“…To detect synchronized bursting events, we scanned the binary data in consecutive windows of 2 s. Each window was divided into bins of 200 ms. Only events with >5 active neurons and a delay of >5 s between the previously detected synchronized burst event were analyzed (Raichman and Ben Jacob 2008). Spike activity was quantified by counting the number of spikes per 10 min recording epoch and normalized to the 1 h control measurement preceding the experimental condition.…”

Section: In Vitro Electrophysiological Recordingsmentioning

confidence: 99%

LPS-Induced Microglial Secretion of TNFα Increases Activity-Dependent Neuronal Apoptosis in the Neonatal Cerebral Cortex

Nimmervoll¹,

White²,

Yang³

et al. 2012

Cerebral Cortex

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During the pre-and neonatal period, the cerebral cortex reveals distinct patterns of spontaneous synchronized activity, which is critically involved in the formation of early networks and in the regulation of neuronal survival and programmed cell death (apoptosis). During this period, the cortex is also highly vulnerable to inflammation and in humans prenatal infection may have a profound impact on neurodevelopment causing long-term neurological deficits. Using in vitro and in vivo multi-electrode array recordings and quantification of caspase-3 (casp-3)-dependent apoptosis, we demonstrate that lipopolysaccharide-induced inflammation causes rapid alterations in the pattern of spontaneous burst activities, which subsequently leads to an increase in apoptosis. We show that these inflammatory effects are specifically initiated by the microglia-derived pro-inflammatory cytokine tumor necrosis factor a and the chemokine macrophage inflammatory protein 2. Our data demonstrate that inflammation-induced modifications in spontaneous network activities influence casp-3-dependent cell death in the developing cerebral cortex.

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Section: In Vitro Electrophysiological Recordingsmentioning

confidence: 99%

LPS-Induced Microglial Secretion of TNFα Increases Activity-Dependent Neuronal Apoptosis in the Neonatal Cerebral Cortex

Nimmervoll¹,

White²,

Yang³

et al. 2012

Cerebral Cortex

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“…When the bursting behavior is organized in array-wide barrages involving the entire network at the same time, the phenomenon is described in the literature with the name of network burst (van Pelt et al 2004). Network bursts consist of sequences of synchronized single-channel bursts, which spread across all or part of the MEA (Eytan and Marom 2006;Raichman and Ben-Jacob 2008;van Pelt et al 2004). When observed at a coarse timescale, these bursts seem to be highly synchronized, but they actually hide different spatiotemporal structures of activity propagation, which repeat over time (Eytan and Marom 2006;Segev et al 2004).…”

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confidence: 99%

A self-adapting approach for the detection of bursts and network bursts in neuronal cultures

2009

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Dissociated networks of neurons typically exhibit bursting behavior, whose features are strongly influenced by the age of the culture, by chemical/electrical stimulation or by environmental conditions. To help the experimenter in identifying the changes possibly induced by specific protocols, we developed a self-adapting method for detecting both bursts and network bursts from electrophysiological activity recorded by means of micro-electrode arrays. The algorithm is based on the computation of the logarithmic inter-spike interval histogram and automatically detects the best threshold to distinguish between inter- and intra-burst inter-spike intervals for each recording channel of the array. An analogous procedure is followed for the detection of network bursts, looking for sequences of closely spaced single-channel bursts. We tested our algorithm on recordings of spontaneous as well as chemically stimulated activity, comparing its performance to other methods available in the literature.

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“…В современных работах по изучению электрофизиологической активности нейрональных культур in vitro особое внимание уделяется феномену спонтанной пачечной активности [1][2][3][4][5][6][7][8][9][10]. Популяционной пачкой (или просто пачкой) активности культуры называется явление резкого возрастания активности групп нейронов в течение короткого промежутка времени.…”

Section: Introductionunclassified

“…При этом каждый электрод регистрирует активность нескольких близлежащих нейронов. Одним из способов анализа активности является учет задержки времени появления первого спайка на каждом из электродов относительно начала популяционной пачки [2]. Было обнаружено [3], что паттерн временных задержек первых спайков на электродах воспроизводится в последовательных пачках активности культуры.…”

Section: Introductionunclassified

Simulation of Spontaneous Activity in Neuronal Cultures with Long-Term Plasticity

Degterev¹

2015

Math.Biol.Bioinf.

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Лаборатория нейроинтеллекта и нейроморфных систем Курчатовский НБИКС-центр, НИЦ "Курчатовский институт", Москва, Россия Аннотация. Широко исследуемым феноменом спонтанной активности культур нейронов in vitro является популяционная пачечная активность. Текущие модели сетевой активности в нейрональных культурах рассматривают такие механизмы генерации пачек, как синаптический шум и присутствие пейсмейкерных нейронов в сети. При этом изменение весов связей между нейронами происходит в соответствии с моделью кратковременной, но не долговременной пластичности, а значит и структура сети, остается неизменной. В данной работе воспроизводятся известные из литературы модели нейронных сетей со статичными синапсами, а затем исследуется изменение спонтанной активности нейронных сетей при учете механизмов долговременной синаптической пластичности. Результаты моделирования показывают, что введение в модель долговременной пластичности приводит к рассогласованию с экспериментальными данными.Ключевые слова: нейрональная культура, синаптическая пластичность, спайк.

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Identifying repeating motifs in the activation of synchronized bursts in cultured neuronal networks

Cited by 91 publications

References 49 publications

LPS-Induced Microglial Secretion of TNFα Increases Activity-Dependent Neuronal Apoptosis in the Neonatal Cerebral Cortex

LPS-Induced Microglial Secretion of TNFα Increases Activity-Dependent Neuronal Apoptosis in the Neonatal Cerebral Cortex

A self-adapting approach for the detection of bursts and network bursts in neuronal cultures

Simulation of Spontaneous Activity in Neuronal Cultures with Long-Term Plasticity

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