Identifying salivary antigens of Phlebotomus argentipes by a 2DE approach

Martín-Martín, Inés; Molina, Ricardo; Jiménez, Maribel

doi:10.1016/j.actatropica.2013.02.008

Cited by 22 publications

(21 citation statements)

References 57 publications

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“…Specific antibodies from dogs bitten by P. perniciosus in the field, as well as under laboratory conditions, reacted strongly with two salivary apyrases [63]. Apyrases from P. perniciosus , P. papatasi , and P. argentipes saliva were also recognized by sera of mice and hamsters immunized by homologous antigen [5], [60], [82]. Furthermore, bacterially expressed recombinant P. duboscqi apyrase (ABI20147) was also recognized by specific antibodies from mice immunized with P. duboscqi saliva [80], suggesting that antibody recognition is not solely targeted to the glycosylated parts of the antigen.…”

Section: Resultsmentioning

confidence: 94%

“…D7-related proteins are highly antigenic and were recognized by specific antibodies from the sera of repeatedly bitten hosts, regardless of natural [15], [61], [63], [67] or experimental exposure [5], [60], [62], [63], [82]. Recombinant P. ariasi D7-related protein (AAX55749) elicited the production of specific humoral response in immunized mice [27].…”

Section: Resultsmentioning

confidence: 99%

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Comparative Analysis of Salivary Gland Transcriptomes of Phlebotomus orientalis Sand Flies from Endemic and Non-endemic Foci of Visceral Leishmaniasis

et al. 2014

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BackgroundIn East Africa, Phlebotomus orientalis serves as the main vector of Leishmania donovani, the causative agent of visceral leishmaniasis (VL). Phlebotomus orientalis is present at two distant localities in Ethiopia; Addis Zemen where VL is endemic and Melka Werer where transmission of VL does not occur. To find out whether the difference in epidemiology of VL is due to distant compositions of P. orientalis saliva we established colonies from Addis Zemen and Melka Werer, analyzed and compared the transcriptomes, proteomes and enzymatic activity of the salivary glands.Methodology/Principal FindingsTwo cDNA libraries were constructed from the female salivary glands of P. orientalis from Addis Zemen and Melka Werer. Clones of each P. orientalis library were randomly selected, sequenced and analyzed. In P. orientalis transcriptomes, we identified members of 13 main protein families. Phylogenetic analysis and multiple sequence alignments were performed to evaluate differences between the P. orientalis colonies and to show the relationship with other sand fly species from the subgenus Larroussius. To further compare both colonies, we investigated the humoral antigenicity and cross-reactivity of the salivary proteins and the activity of salivary apyrase and hyaluronidase.ConclusionsThis is the first report of the salivary components of P. orientalis, an important vector sand fly. Our study expanded the knowledge of salivary gland compounds of sand fly species in the subgenus Larroussius. Based on the phylogenetic analysis, we showed that P. orientalis is closely related to Phlebotomus tobbi and Phlebotomus perniciosus, whereas Phlebotomus ariasi is evolutionarily more distinct species. We also demonstrated that there is no significant difference between the transcriptomes, proteomes or enzymatic properties of the salivary components of Addis Zemen (endemic area) and Melka Werer (non-endemic area) P. orientalis colonies. Thus, the different epidemiology of VL in these Ethiopian foci cannot be attributed to the salivary gland composition.

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Section: Resultsmentioning

confidence: 94%

Section: Resultsmentioning

confidence: 99%

Comparative Analysis of Salivary Gland Transcriptomes of Phlebotomus orientalis Sand Flies from Endemic and Non-endemic Foci of Visceral Leishmaniasis

et al. 2014

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“…Moreover, differences in post-translational modifications of salivary proteins, such as glycosylation or phosphorylation patterns,44 between the Tunisian and Israeli colonies may have led to differences in protein identification and even antibody specificities. Both studies used manual scoring of immunoblots, and designations of positive specificities at particular MWs may not be consistent between studies (i.e., what our observers agreed was specificity at 26 and 38 kDa may have been, for Marzouki and others12 observers, specificity at 28 and 36 kDa).…”

Section: Discussionmentioning

confidence: 99%

Profiling of Human Acquired Immunity Against the Salivary Proteins of Phlebotomus papatasi Reveals Clusters of Differential Immunoreactivity

Geraci¹,

Mukbel²,

Kemp³

et al. 2014

The American Society of Tropical Medicine and Hygiene

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Phlebotomus papatasi sand flies are among the primary vectors of Leishmania major parasites from Morocco to the Indian subcontinent and from southern Europe to central and eastern Africa. Antibody-based immunity to sand fly salivary gland proteins in human populations remains a complex contextual problem that is not yet fully understood. We profiled the immunoreactivities of plasma antibodies to sand fly salivary gland sonicates (SGSs) from 229 human blood donors residing in different regions of sand fly endemicity throughout Jordan and Egypt as well as 69 US military personnel, who were differentially exposed to P. papatasi bites and L. major infections in Iraq. Compared with plasma from control region donors, antibodies were significantly immunoreactive to five salivary proteins (12, 26, 30, 38, and 44 kDa) among Jordanian and Egyptian donors, with immunoglobulin G4 being the dominant anti-SGS isotype. US personnel were significantly immunoreactive to only two salivary proteins (38 and 14 kDa). Using k-means clustering, donors were segregated into four clusters distinguished by unique immunoreactivity profiles to varying combinations of the significantly immunogenic salivary proteins. SGS-induced cellular proliferation was diminished among donors residing in sand fly-endemic regions. These data provide a clearer picture of human immune responses to sand fly vector salivary constituents.

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“…Nucleotide and amino acid sequences were retrieved from the NCBI databases from sand fly salivary gland transcriptomes (Valenzuela et al, 2001, 2004; Anderson et al, 2006; Oliveira et al, 2006; Hostomská et al, 2009; Abdeladhim et al, 2012, 2016; Rohousova et al, 2012; de Moura et al, 2013; Kato et al, 2013; Martín-Martín et al, 2013; Vlkova et al, 2014). Signal peptides were removed from the protein sequences whereas sequences encoding signal peptides and stop codons were removed from the nucleotide sequences for further analyses.…”

Section: Methodsmentioning

confidence: 99%

“…From the OW group, 10 species have had their salivary transcriptomes decoded. These species belong to five subgenera of the genus Phlebotomus, including the subgenera Phlebotomus, Paraphlebotomus, Larroussius, Adlerius, and Euphlebotomus (Valenzuela et al, 2001; Anderson et al, 2006; Kato et al, 2006; Oliveira et al, 2006; Hostomská et al, 2009; Abdeladhim et al, 2012; Rohousova et al, 2012; Martín-Martín et al, 2013; Vlkova et al, 2014). For NW sand flies, salivary gland transcriptomes were sequenced from four species.…”

Section: Introductionmentioning

confidence: 99%

Comparative Evolution of Sand Fly Salivary Protein Families and Implications for Biomarkers of Vector Exposure and Salivary Vaccine Candidates

Coutinho-Abreu

Valenzuela

2018

Front. Cell. Infect. Microbiol.

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Sand fly salivary proteins that produce a specific antibody response in humans and animal reservoirs have been shown to be promising biomarkers of sand fly exposure. Furthermore, immunity to sand fly salivary proteins were shown to protect rodents and non-human primates against Leishmania infection. We are missing critical information regarding the divergence amongst sand fly salivary proteins from different sand fly vectors, a knowledge that will support the search of broad or specific salivary biomarkers of vector exposure and those for vaccines components against leishmaniasis. Here, we compare the molecular evolution of the salivary protein families in New World and Old World sand flies from 14 different sand fly vectors. We found that the protein families unique to OW sand flies are more conserved than those unique to NW sand flies regarding both sequence polymorphisms and copy number variation. In addition, the protein families unique to OW sand flies do not display as many conserved cysteine residues as the one unique to the NW group (28.5% in OW vs. 62.5% in NW). Moreover, the expression of specific protein families is restricted to the salivary glands of unique sand fly taxon. For instance, the ParSP15 family is unique to the Larroussius subgenus whereas phospholipase A2 is only expressed in member of Larroussius and Adlerius subgenera. The SP2.5-like family is only expressed in members of the Phlebotomus and Paraphlebotomus subgenera. The sequences shared between OW and NW sand flies have diverged at similar rates (38.7 and 45.3% amino acid divergence, respectively), yet differences in gene copy number were evident across protein families and sand fly species. Overall, this comparative analysis sheds light on the different modes of sand fly salivary protein family divergence. Also, it informs which protein families are unique and conserved within taxon for the choice of taxon-specific biomarkers of vector exposure, as well as those families more conserved across taxa to be used as pan-specific vaccines for leishmaniasis.

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Identifying salivary antigens of Phlebotomus argentipes by a 2DE approach

Cited by 22 publications

References 57 publications

Comparative Analysis of Salivary Gland Transcriptomes of Phlebotomus orientalis Sand Flies from Endemic and Non-endemic Foci of Visceral Leishmaniasis

Comparative Analysis of Salivary Gland Transcriptomes of Phlebotomus orientalis Sand Flies from Endemic and Non-endemic Foci of Visceral Leishmaniasis

Profiling of Human Acquired Immunity Against the Salivary Proteins of Phlebotomus papatasi Reveals Clusters of Differential Immunoreactivity

Comparative Evolution of Sand Fly Salivary Protein Families and Implications for Biomarkers of Vector Exposure and Salivary Vaccine Candidates

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