“…For example, a number of growth-regulating factors (such as Fibroblast Growth Factor (FGF), Hepatocyte Growth Factor (HGF), Leukemia Inhibitory Factor (LIF), Vascular Endothelial Growth Factor C (VEGF-C) and transferrin) have been identified in spent-culture media of CHO cells, whose supplementation in the culture media enhances the cell growth in culture (~48%) even at low cell density and improves the performance of production culture [4]. On the other hand, a number of proteolytic enzymes (matrix metallopeptidase 3 (MMP3), MMP10, MMP12 and cathepsin-B) have been identified in culture media which may pose risk for proteolytic-degradation of the product leading to low yield from production batches [5,6]. Besides, efficient removal of these peptides/proteins along with other Host Cell Proteins (HCPs) from the final product during down-stream processing is mandatory for product safety and/or longer shelf-life.…”