Identifying the molecular targets and mechanisms of xuebijing injection for the treatment of COVID-19 via network pharmacology and molecular docking

Tian-yu, Zhao; Guan, Liying

doi:10.1080/21655979.2021.1933301

Cited by 18 publications

(14 citation statements)

References 24 publications

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“…To fully evaluate the curative effects and mechanisms of Quercetin in treating CRF scientifically, the present study established a CRF rat model and intragastrically administered Quercetin to determine its effects. We constructed a regulatory network of SQJZJN-Quercetin-PIK3R1-CRF in a network pharmacological approach, investigated the target functions and molecular regulatory mechanisms to clarify the mechanisms of Quercetin on alleviating or delaying renal fibrosis [ 10 ] . The present study aimed to explore the mechanisms in the incidence and development of CRF, the clinical efficacy of SQJZJN and Quercetin, and the mechanisms of alleviating renal fibrosis.…”

Section: Introductionmentioning

confidence: 99%

Quercetin alleviates chronic renal failure by targeting the PI3k/Akt pathway

Luo

et al. 2021

Bioengineered

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Chronic renal failure (CRF) threatens human health greatly and attracts worldwide concerns of health professionals in the public health sector. In our preliminary study, we found that Compound capsule (Shengqing Jiangzhuo Capsule, SQJZJN) had a significant therapeutic effect on CRF. Quercetin is one of the main components of this Compound capsule. In this study, we investigated the effect of Quercetin monomer on CRF and the regulation of PI3k/Akt pathway. Network pharmacology analysis methods were employed to analyze the SQJZJN/Quercetin/PIK3R1 network relationships. In this study, a CRF rat model was prepared using the gavage adenine solution method and detected the indicators of Creatinine (Cr), Blood Urea Nitrogen (BUN), and Uric Acid (UA). After treating the rat model with Quercetin and PIK3R1-interfering lentivirus, respectively, we observed the changes on the histological morphology of the kidney and detected apoptosis using TUNEL staining. Gene and protein expression associated with renal function were detected using qPCR, WB and immunofluorescence. Quercetin was identified as the main ingredient of SQJZJN by the network pharmacological screening and Quercetin at 1.5 and 3 g/(kg.d) concentrations could effectively alleviate the CRF symptoms, reduce the levels of Cr, BUN, and UA, and markedly inhibit cell apoptosis demonstrated by the intragastric administration. Furthermore, the protein expression of p-PI3K, p-AKT, NLRP3, caspase1, AQP1, and AQP2 in all groups was detected by immunofluorescence and western blot assays, indicating that Quercetin could reduce the expression of NLRP3, caspase1, p-PI3k, and p-Akt, and increase the expression of AQP1 and AQP2 in the renal tissues of CRF rats. Being labeled with biotin and incubated with the total protein extracted from kidney tissues, Quercetin could bind to PIK3R1. Following the PIK3R1 interference lentivirus was injected into the CRF model rats by tail vein, the CRF symptoms were effectively alleviated in the PIK3R1 interference group, consistent with the effect of Quercetin. Taken together, Quercetin, a major component of SQJZJN, might minimize renal fibrosis and apoptosis in CRF rats by inhibiting the PI3k/Akt pathway through targeting PIK3R1. By regulating AQP1 and AQP2, both water retention and toxin accumulation were reduced.

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Section: Introductionmentioning

confidence: 99%

Quercetin alleviates chronic renal failure by targeting the PI3k/Akt pathway

Luo

et al. 2021

Bioengineered

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“…Other clinical studies also found that the NF- κ B activity was increased in NASH patients. AKT regulates NF- κ B signal transduction through phosphorylation of IKK α , which leads to the degradation of I κ B [ 34 ]. This process releases NF- κ B from the cytoplasm into the nucleus to further regulate the expression of downstream target genes and finally mediate multiple pathological processes such as liver inflammation and apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Pharmacological Mechanism of Ganlu Powder in the Treatment of NASH Based on Network Pharmacology and Molecular Docking

et al. 2022

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Nonalcoholic steatohepatitis (NASH), a progression of nonalcoholic fatty liver disease (NAFLD), is a clinical syndrome characterized by liver steatosis, inflammation, and hepatocellular damage. Ganlu powder (GLP) is a classic traditional Chinese medicine prescription that has shown favorable treatment effects on NASH. However, the underlying therapeutic mechanisms are still poorly understood. This study is aimed at exploring the potential mechanism of GLP in the treatment of NASH via network pharmacology and molecular docking. PubMed and CNKI databases were used to identify the components of GLP. Swiss and STITCH databases were employed to obtain corresponding drug targets. NASH targets were adopted from the Therapeutic Target Database (TTD), DisGeNET, DrugBank, GeneCards, and MalaCards databases. Cytoscape software was utilized to construct “drug-ingredient-target-disease” networks and the protein-protein interaction (PPI) network of GLP in NASH. AKT1 was identified as the key target. The GO functional enrichment analysis revealed that GLP might treat NASH by modulating the inflammatory response and regulating phosphatidylinositol 3-kinase signaling. The KEGG analysis showed that GLP might treat NASH by regulating the tumor necrosis factor (TNF) signal pathway by affecting the role of AKT1. According to the network pharmacology results, a virtual docking of active compounds with AKT1 was carried out, and the results indicated that the 7 components, berberine, epiberberine, jatrorrhizine, coptisine, palmatine, evodiamine, and rutecarpine, can bind stably with AKT1 and have higher binding energy than AKT1 inhibitors. The overall study findings suggest that GLP may treat NASH by regulating AKT1.

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“…COVID-19 disease caused by SARS-CoV-2, which spreads worldwide from Wuhan, China, and caused %2 of the death of COVID-19 patients as an average mortality rate worldwide, is a member of the beta coronavirus family (1,3,(11)(12)(13). It is reported that during the pandemic process, a lot of scientific researches was carried out, in particular, the molecular and biological mechanisms of COVID-19 as well as diagnosis and treatment (14)(15)(16)(17)(18)(19)(20)(21)(22). Analyzing the literature related to COVID-19 helps us to evaluate the pathophysiologic mechanism of the disease and understand its progression.…”

Section: Discussionmentioning

confidence: 99%

How did research article publications on the COVID-19 pandemic progress in the Q1 ranked SCImage index journals in 2020?

Daş¹,

Buğra²

2022

Journal of Health Sciences and Medicine

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Aim:The aim of this paper is to survey the COVID-19 research articles in Q1 ranked high SJR index journals according to the SCImago journal rank indicator. Material and Method:The study was carried out on the website named "https://www.scimagojr.com". The search was conducted by selecting the criteria "medicine", "pathology and forensic medicine", "all regions/countries", "journals", and the "2020" as the year. Only 50 scientific journals met these criteria. COVID-19-related and unrelated research articles published in these journals were manually scanned. Review articles, book reviews, conferences, commentaries, case reports, mini reviews, short communications, letters to the editor were not included in the study. COVID-19 related research articles were divided into groups in terms of antemortem and postmortem type of the study by reviewing the abstract of the studies and also grouped according to the country of first author and countries of all authors. Results: A total of 3906 research articles published in 50 journals with Q1 SJR index over 0.887 were investigated. Of these 3906 research articles, 40 of them were related to COVID-19. 31 of these 40 COVID-19 related research articles were antemortem and 9 were postmortem studies. Among these 40 COVID-19 related scientific articles, the first author of publications belonged to 12 countries. United States, Australia, China, and Italy were found to be the most productive countries for published research articles on COVID-19 in selected high SJR index journals. The United States was the country with the highest number of first authors with 15 articles. China was the country with the highest number of multinational author list research articles. Conclusion:We believe that the number and content of studies conducted in these high-quality scientific journals on COVID-19 make important contributions and understanding in the fields of disease transmission, disease prevention, course and severity of symptoms, pathophysiology, molecular characteristics, and treatment approach processes.

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Identifying the molecular targets and mechanisms of xuebijing injection for the treatment of COVID-19 via network pharmacology and molecular docking

Cited by 18 publications

References 24 publications

Quercetin alleviates chronic renal failure by targeting the PI3k/Akt pathway

Quercetin alleviates chronic renal failure by targeting the PI3k/Akt pathway

Pharmacological Mechanism of Ganlu Powder in the Treatment of NASH Based on Network Pharmacology and Molecular Docking

How did research article publications on the COVID-19 pandemic progress in the Q1 ranked SCImage index journals in 2020?

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