IgE-Binding Epitope Mapping and Tissue Localization of the Major American Cockroach Allergen Per a 2

Lee, Mey Fann; Chang, Chia Wei; Song, Pei Pong; Hwang, Guang-Yuh; Lin, Shyh Jye; Chen, Yi Hsing

doi:10.4168/aair.2015.7.4.376

Cited by 9 publications

(6 citation statements)

References 35 publications

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“…In Taiwan, airway allergic patients sensitized to Per a 2 had more severe allergy and elevated proinflammatory chemokines [39]. Secretion of group 2 allergens into the gut has been demonstrated for Bla g 2 by immunoassays of dissected cockroach body parts and tissues, and for Per a 2 by immunohistochemical staining [24,83]. Eventually, allergens from group 2 (and group 1) are excreted to the environment, a fact that, combined with their molecular stability, makes them excellent markers of cockroach allergen exposure.…”

Section: Cockroach Allergens: a Heterogeneous Group Of Proteinsmentioning

confidence: 99%

New Insights into Cockroach Allergens

Pomés

Mueller

Randall

et al. 2017

Curr Allergy Asthma Rep

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Purpose of review This review addresses the most recent developments on cockroach allergen research in relation to allergic diseases, especially asthma. Recent findings The number of allergens relevant to cockroach allergy has recently expanded considerably up to 12 groups. New X-ray crystal structures of allergens from groups 1, 2 and 5 revealed interesting features with implications for allergen standardization, sensitization, diagnosis and therapy. Summary Cockroach allergy is strongly associated with asthma particularly among children and young adults living in inner-city environments, posing challenges for disease control. Environmental interventions targeted at reducing cockroach allergen exposure have provided conflicting results. Immunotherapy may be a way to modify the natural history of cockroach allergy and decrease symptoms and asthma severity among sensitized and exposed individuals. The new information on cockroach allergens is important for the assessment of allergen markers of exposure and disease, and for the design of immunotherapy trials.

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Section: Cockroach Allergens: a Heterogeneous Group Of Proteinsmentioning

confidence: 99%

New Insights into Cockroach Allergens

Pomés

Mueller

Randall

et al. 2017

Curr Allergy Asthma Rep

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“…In the present study, we constructed a TuMV infectious plant virus to express American cockroach major allergen Per a 2 in Chinese cabbage using a full-clone major allergen Per a 2-996 (containing all IgE binding epitopes) and a partial clone allergen Per a 2-372 that containing only one IgE-binding epitope from our previous study (31). In this murine allergic asthma model, though pre-treated with allergen-or hypoallergen-containing cabbage blunt the development of allergen-specific IgE and IL-4 mRNA expression after challenge, only the hypoallergen, Per a 2-372 had preventive effects on airway hyperresponsiveness and inflammation.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…We previously identified three linear IgE-binding epitopes on Per a 2 [31]. On this basis, we generated a recombinant hypoallergenic clone 372 of Per a 2, which containing only one IgEbinding epitope of Per a 2 sequence 200-211 [31], as the candidate for a low-risk immunotherapy vaccine. In this study, we examined whether edible plant vaccine encoding Per a 2 fulllength clone 996 or its hypoallergenic clone 372 could exert a prophylactic effect in roach-allergic asthmatic mice.…”

Section: Introductionmentioning

confidence: 99%

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Oral edible plant vaccine containing hypoallergen of American cockroach major allergen Per a 2 prevents roach-allergic asthma in a murine model

Lee

Chiang

et al. 2018

PLoS ONE

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BackgroundAmerican cockroaches (Periplaneta americana) are an important indoor allergen source and a major risk factor for exacerbations and poor control of asthma. We previously reported that allergen components from American cockroaches exhibit varying levels of pathogenicity. Sensitization to major American cockroach allergen, Per a 2, correlated with more severe clinical phenotypes among patients with allergic airway diseases.Materials and methodsIn this study, we examined whether oral plant vaccine-encoding full-length Per a 2 clone-996 or its hypoallergenic clone-372 could exert a prophylactic role in Per a 2-sensitized mice. The cDNAs coding Per a 2–996 and Per a 2–372 were inserted into TuMV vector and expressed in Chinese cabbage. Adult female BALB/c mice were fed with the cabbage extracts for 21 days and subsequently underwent two-step sensitization with recombinant Per a 2.ResultsPer a 2-specific IgE measured by in-house ELISA in the sera of Per a 2-372-treated groups were significantly lower than in the control groups after allergen challenge but not the Per a 2-996-treated group. Moreover, Per a 2–372 vaccine markedly decreased airway hyper-responsiveness and infiltration of inflammatory cells into the lungs, as well as reduced mRNA expression of IL-4 and IL-13 in comparison with the control mice.ConclusionOur data suggest that oral administration of edible plant vaccine encoding Per a 2 hypo-allergen may be used as a prophylactic strategy against the development of cockroach allergy.

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“…The identification of B cell epitopes (IgE-binding epitopes) of an allergen is helpful in designing sequences for more accurate and safer peptide-based allergen diagnosis and immunotherapeutic agents. The IgE-binding epitopes of Per a 1 (21), Per a 2 (22), Per a 3 (23), Per a 4 (8) and Per a 6 (10) have previously been identified by experimental or bioinformatics methods. T cell epitopes have been successfully identified based on computer simulation over the past decade.…”

Section: Introductionmentioning

confidence: 99%

In silico epitope prediction, expression and functional analysis of Per a 10 allergen from the American cockroach

Tong

Guo

Jin

et al. 2016

International Journal of Molecular Medicine

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Cockroach (CR) allergies caused by the American cockroach hyave been recognized to be repsonsible for IgE-mediated type I hypersensitivity worldwide. Per a 10 is one of the recognized main allergens of the American CR. In a previous study, we examined another American CR allergen, Per a 9 in patients with CR allergies and examined epitope sequences in this allergen. In the present study, we aimed to examine epitope sequences in the Per a 10 allergen. for this purpose, the Per a 10 gene was cloned and expressed in Escherichia coli (E. coli) systems. Our results revealed that 9 out of 16 (56.3%) sera from patients with American CR allergies reacted to Per a10, as assessed by ELISA, confirming that Per a 10 is a major allergen of the American CR. Our results also revealed that the expression of CD63 and CCR3 on passively sensitized basophils (obtained sera of patients with American CR allergies) was increased by approximately 2.3-fold, indicating that recombinant Per a 10 is functionally active. In addition, 3 immunoinformatics tools, namely the DNAStar Protean system, the Bioinformatics Predicted Antigenic Peptides (BPAP) system and the BepiPred 1.0 server were used to predict the peptides and the results revealed 8 peptides (2–12, 55–67, 98–120, 125–133, 149–160, 170–182, 201–208 and 223–227) as potential B cell epitopes of the Per a 10 allergen. Moreover, Per a 10 was predicted to have 3 T cell epitope sequences, namely 83–92, 139–147 and 162–170. The findings of our study on the CR allergen may prove to be useful in the development of peptide-based vaccine for the prevention and/or treatment of CR allergies.

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IgE-Binding Epitope Mapping and Tissue Localization of the Major American Cockroach Allergen Per a 2

Cited by 9 publications

References 35 publications

New Insights into Cockroach Allergens

New Insights into Cockroach Allergens

Oral edible plant vaccine containing hypoallergen of American cockroach major allergen Per a 2 prevents roach-allergic asthma in a murine model

In silico epitope prediction, expression and functional analysis of Per a 10 allergen from the American cockroach

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