IgE Binding to Ovalbumin and Ovomucoid Epitopes in Extensively Heated Egg-Challenged Patients: A Microarray Pilot Study

Leonard, Stephanie A.; Lin, Jason L. J.; Bardina, L.; Goldis, Marina; Nowak-Wegryzn, Anna

doi:10.1016/j.jaci.2009.12.869

Cited by 1 publication

(10 citation statements)

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“…Beyond the magnitude of egg sIgE levels or OVM sIgE levels, a higher reactivity to linear epitopes (that are less heat‐altered) in patients reactive to heated egg might also play a role 33 . This type of information is, however, not obtained by measurement of sIgE binding to the entire allergen and specific epitopes that might predict the tolerance or not to heated egg would need to be confirmed 28 …”

Section: Why Do Some Patients Tolerate Heated Egg?mentioning

confidence: 99%

“…As IgE F I G U R E 1 Overview of published epitopes for Ovalbumin (Gal d 2, OVA). [23][24][25][26][27][28][29][30][31] F I G U R E 2 Overview of published epitopes for Ovomucoid (Gal d 1, OVM). 25,28,29,[32][33][34][35][36] LEAU ET AL.…”

Section: Heating Can Modify Immunoreactive Epitopesmentioning

confidence: 99%

“…[23][24][25][26][27][28][29][30][31] F I G U R E 2 Overview of published epitopes for Ovomucoid (Gal d 1, OVM). 25,28,29,[32][33][34][35][36] LEAU ET AL.…”

Section: Heating Can Modify Immunoreactive Epitopesmentioning

confidence: 99%

“…Indeed, two linear epitopes (aa 229-243, 280-297) were suggested to be specific for patients sensitive to extensively heated egg. 28 For OVM, both linear and conformational epitopes play a role in OVM allergy and their relative importance likely differs per patient. 63 Some OVM-sensitized individuals might not recognize linear epitopes at all.…”

Section: Heating Can Modify Immunoreactive Epitopesmentioning

confidence: 99%

“…Due to the heat lability of OVA, these conformational epitopes are likely lost upon heating. Different linear epitopes have also been identified, although with significant disparity between studies 24–29 . One linear epitope located around aa367‐385 was highlighted by multiple studies and was recognized by 80% of sera from egg‐allergic patients in one study, underlining its immunological importance 23–25,27 .…”

Section: Why Do Some Patients Tolerate Heated Egg?mentioning

confidence: 99%

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Tolerance to heated egg in egg allergy: Explanations and implications for prevention and treatment

Leau,

Denery‐Papini,

Bodinier

et al. 2023

Clinical & Translational All

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Hen's egg allergy is the second most frequent food allergy found in children. Allergic symptoms can be caused by raw or heated egg, but a majority of egg‐allergic children can tolerate hard‐boiled or baked egg. Understanding the reasons for the tolerance towards heated egg provides clues about the molecular mechanisms involved in egg allergy, and the differential allergenicity of heated and baked egg might be exploited to prevent or treat egg allergy. In this review, we therefore discuss (i) why some patients are able to tolerate heated egg; by highlighting the structural changes of egg white (EW) proteins upon heating and their impact on immunoreactivity, as well as patient characteristics, and (ii) to what extent heated or baked EW might be useful for primary prevention strategies or oral immunotherapy. We describe that the level of immunoreactivity towards EW helps to discriminate patients tolerant or reactive to heated or baked egg. Furthermore, the use of heated or baked egg seems effective in primary prevention strategies and might limit adverse reactions. Oral immunotherapy is a promising treatment strategy, but it can sometimes cause significant adverse events. The use of heated or baked egg might limit these, but current literature is insufficient to conclude about its efficacy.

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Section: Why Do Some Patients Tolerate Heated Egg?mentioning

confidence: 99%

Section: Heating Can Modify Immunoreactive Epitopesmentioning

confidence: 99%

“…[23][24][25][26][27][28][29][30][31] F I G U R E 2 Overview of published epitopes for Ovomucoid (Gal d 1, OVM). 25,28,29,[32][33][34][35][36] LEAU ET AL.…”

Section: Heating Can Modify Immunoreactive Epitopesmentioning

confidence: 99%

Section: Heating Can Modify Immunoreactive Epitopesmentioning

confidence: 99%

Section: Why Do Some Patients Tolerate Heated Egg?mentioning

confidence: 99%

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