IgE‐induced histamine release from rat basophilic leukemia cell lines: isolation of releasing and nonreleasing clones

Abstract

The rat basophilic leukemia (RBL) cell lines were cloned and the various sublines compared for their chromosome number, IgE-mediated histamine release and for IgE surface receptors. It was found that cell lines started from tumors at different times vary in both their chromosome number and their ability to release histamine by an IgE-mediated reaction. RBL-I and III have approximately 44 chromosomes and did not respond to an IgE-mediated reaction. RBL-II and RBL-IV have 68-73 chromosomes and showed moderate levels of histamine release (percent release mean = 5 +/- 2 and 10 +/- 4, respectively). The cloning of the RBL-IV line resulted in some sublines which were excellent histamine releasers (range 39-100%) and some which were relatively refractory (less than 10%) to IgE-mediated histamine release. These clones did not differ significantly in chromosome number. Recloning the releasing lines gave rise to poor releasers, whereas the recloning of poor releasers did not produce good releasers indicating that the mutational drift in culture is toward loss of histamine-releasing capacity. The number of IgE receptors and the rate of IgE association and dissociation were similar for the different cell lines. The study failed to disclose significant molecular weight differences in the IgE receptor from the various clones and sublines indicating that the failure to release probably does not reside in the receptor. The various cloned sublines are phenotypically stable, and the isolation of excellent histamine-releasing sublines are useful for studies of the complex phenomenon of the histamine release.

Stimulation of tryptophan hydroxylase production in a serotonin‐producing cell line (RBL2H3) by intracellular calcium mobilizing reagents

“…RBL2H3 cells retain this ability to respond [14,15]; the cells discharged about 20% of the cellular serotonin (total: = 1.75 nmol/106 cells) within 1 h. A calcium ionophore A23187 (0.08 ~tM) caused serotonin release by RBL cells similar to that described for histamine release [10]. After immune stimulation (DNP-BSA/IgE), the TPH activity of RBL2H3 cells increased up to about 9-fold (Fig.…”

Calcium Signals in Rat Basophilic Leukemia (RBL-2H3) Cells Primed with the Neuropeptide Substance P.

“…We have recently shown that direct nerve-mast cell (RBL) cross-talk can occur in the absence of an intermediary transducing cell and that the neuropeptide substance P is an important mediator of this communication. 6,17) It is known that RBL cells have the properties of MMCs and do not respond to substance P. 18,19) We tried to stimulate wild-type RBL cells with substance P (0.1-10 mM), but they did not respond. However, the addition of purified neutralizing anti-substance P to the co-culture milieu of the neurite-RBL system dose dependently prevented RBL activation elicited by bradykinin or scorpion venom stimulation of neurites, but did not have any direct effect on neuronal activation.…”

GD1b-Derived Gangliosides Modulate FcεRI Endocytosis in Mast Cells

“…The E5 cells express 82.9% and D1 cells express <1% of GM1 in relation to the amount in RBL-2H3 cells (Silveira e Souza et al 2010). All cell lines used in these experiments were grown as monolayers, as previously described (Barsumian et al 1981).…”