IgE is present on peripheral blood monocytes and B cells in normal dogs and dogs with atopic dermatitis but there is no correlation with serum IgE concentrations

Jackson, Hilary A.; Orton, Susan M.; Hammerberg, Bruce

doi:10.1016/s0165-2427(02)00003-x

Cited by 17 publications

(17 citation statements)

References 24 publications

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“…The relative increase in the number of T-lymphocytes in dogs diagnosed with AD was in accordance with the findings of other authors (Jackson et al 2002;Taszkun 2013). The observation that the proportion of this subpopulation was increasing, while the absolute number remained at the same level, was due to the decline in other subpopulations, particularly B-lymphocytes.…”

Section: Discussionsupporting

confidence: 92%

“…The haematological results obtained in our study can be compared with other studies conducted thus far, which monitored changes in the peripheral blood of dogs affected with AD (Olivry et al 1997;Griffin and DeBoer 2001;Jackson et al 2002;Maeda et al 2004;Maeda et al 2007;Taszkun 2013). The reduction in the relative proportion of lymphocytes was likely a result of an increasing number of neutrophils in response to a potential secondary infection in patients with a severe form of the disease or in response to long-term exposure to stress.…”

Section: Discussionmentioning

confidence: 55%

“…The number of B-lymphocytes in the peripheral blood of canine patients has been reported in some studies (Faldyna and Toman 1998;Jackson et al 2002;Taszkun 2013). The changes reported by Jackson et al (2002) were non-significant and Taszkun (2013) observed a higher number of these cells in dogs with AD complicated by purulent dermatitis.…”

Section: Discussionmentioning

confidence: 72%

“…The changes reported by Jackson et al (2002) were non-significant and Taszkun (2013) observed a higher number of these cells in dogs with AD complicated by purulent dermatitis. This decrease could be explained by the cell-mediated response in dogs with AD in our study.…”

Section: Discussionmentioning

confidence: 92%

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Changes in leukocyte counts, lymphocyte subpopulations and the mRNA expression of selected cytokines in the peripheral blood of dogs with atopic dermatitis

Hoskova¹,

Svoboda²,

Satinska³

et al. 2015

Vet. Med. - Czech

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Canine atopic dermatitis (CAD) is a chronic allergic disease characterised by genetic predisposition. The exact pathogenesis of the disease is still not fully understood. Blood parameters, lymphocyte subpopulations, cytokines and other molecules involved in pro-and anti-inflammatory activities, and Th1/Th2 polarisation in peripheral blood were assessed in clinically healthy dogs and dogs with AD by haematological examination, flow cytometry and qRT-PCR. The group of diseased dogs was further divided into subgroups according to the severity of clinical signs expressed as CADESI-03 (canine atopic dermatitis extent and severity index) values. The group of dogs with CAD showed mild neutrophilia without general leucocytosis, reduced numbers of B-lymphocytes (P < 0.05), increased relative numbers of T-lymphocytes (P < 0.01) decreased absolute numbers of double-positive lymphocytes (P < 0.001) and NK cells (P < 0.05) as compared with healthy controls. The expression levels of IL-10 mRNA were significantly higher (P < 0.05) in the group with CAD. Correlation with CADESI-03 was observed in expression levels of CCL3 (P < 0.05), CCL5 (P < 0.05) and SOCS-3 mRNAs (P < 0.05). In conclusion, the disease stage investigated in the present study is characterised by a chronic inflammatory response.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 55%

Section: Discussionmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 92%

See 2 more Smart Citations

Changes in leukocyte counts, lymphocyte subpopulations and the mRNA expression of selected cytokines in the peripheral blood of dogs with atopic dermatitis

Hoskova¹,

Svoboda²,

Satinska³

et al. 2015

Vet. Med. - Czech

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“…12 Aggregation of this IgE by the intradermal injection of cross-linking anti-canine IgE antibodies has recently been demonstrated to produce immediate reactions and late-phase reactions (LPR) in the skin of both normal dogs and dogs with naturally occurring AD. 13 These reactions grossly and microscopically resemble those generated by the intradermal injection of allergen or anti-IgE in humans with atopic disorders, including AD.…”

Section: M M U N O L O G Y O R I G I N a L A R T I C L Ementioning

confidence: 99%

A canine model of cutaneous late‐phase reactions: prednisolone inhibition of cellular and cytokine responses

Pucheu‐Haston

Shuster²,

Olivry

et al. 2005

Immunology

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SummaryImmunoglobulin E (IgE)-mediated late-phase reactions can be induced in atopic humans by intradermal injection of relevant allergens or anti-IgE antibodies. The histology of these reactions resembles that of naturally occurring atopic dermatitis. Strikingly similar responses can be induced in dogs, suggesting that a canine model could prove valuable for preclinical investigation of drugs targeting late-phase reactions. This study was designed to characterize the cellular, cytokine and chemokine responses after intradermal anti-IgE injection in untreated and prednisolone-treated dogs. Normal beagles were untreated or treated with prednisolone before intradermal injection of polyclonal rabbit anti-canine IgE or normal rabbit IgG. Biopsies were taken before injection and 6, 24 and 48 hr after injection. Samples were evaluated by histological and immunohistochemical staining, as well as by real-time quantitative polymerase chain reaction analysis. Dermal eosinophil and neutrophil numbers increased dramatically within 6 hr after injection of rabbit anti-canine IgE, and remained moderately elevated at 48 hr. The numbers of CD1c + and CD3 + mononuclear cells were also increased at 6 hr. The real-time quantitative polymerase chain reaction demonstrated marked increases in mRNA expression for interleukin-13 (IL-13), CCL2, CCL5 and CCL17. Levels of mRNA for IL-2, IL-4, IL-6 and IFN-c did not change within the limits of detection. Prednisolone administration suppressed the influx of neutrophils, eosinophils, CD1c + and CD3 + cells, as well as expression of IL-13, CCL2, CCL5 and CCL17. These data document the cytokine and chemokine responses to anti-IgE injection in canine skin, and they demonstrate the ability of the model to characterize the anti-inflammatory effects of a known therapeutic agent.

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Generation of therapeutic antibody responses against IgE in dogs, an animal species with exceptionally high plasma IgE levels

Ledin

Bergvall

Hillbertz

et al. 2006

Vaccine

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IgE is present on peripheral blood monocytes and B cells in normal dogs and dogs with atopic dermatitis but there is no correlation with serum IgE concentrations

Cited by 17 publications

References 24 publications

Changes in leukocyte counts, lymphocyte subpopulations and the mRNA expression of selected cytokines in the peripheral blood of dogs with atopic dermatitis

Changes in leukocyte counts, lymphocyte subpopulations and the mRNA expression of selected cytokines in the peripheral blood of dogs with atopic dermatitis

A canine model of cutaneous late‐phase reactions: prednisolone inhibition of cellular and cytokine responses

Generation of therapeutic antibody responses against IgE in dogs, an animal species with exceptionally high plasma IgE levels

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