IgE responses in the serum and gastric lymph of sheep infected with <i>Teladorsagia circumcincta</i>

Huntley, John F.; Schallig, Henk D. F. H.; Kooyman, F.N.J.; Mackellar, A.; Millership, Jason J.; Smith, William D.

doi:10.1046/j.1365-3024.1998.00137.x

Cited by 16 publications

(12 citation statements)

References 25 publications

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“…Studies on IgE response in ruminant were as yet restricted to sheep and cattle. Thus, in sheep increased total and nematode-specific serum IgE levels after gastrointestinal nematode infection have been reported in many studies (Huntley et al, 1998;Pernthaner et al, 2005). Furthermore, a negative correlation between worm counts and total serum IgE levels was found in sheep parasitized with H. contortus (Kooyman et al, 1997).…”

Section: Discussionmentioning

confidence: 71%

Serum antibody responses in Creole kids experimentally infected with Haemonchus contortus

Bambou¹,

Chevrotière²,

Varo³

et al. 2008

Veterinary Parasitology

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The objective of this study was to evaluate the relationship of parasite-specific serum antibodies with the resistance status of Creole kids. The average breeding values on egg output predicted in a context of natural infection at 11 months of age were distant of 1.07 genetic standard deviation between resistant and susceptible animals. After drenching the animals were maintained worm-free during 1 month until experimental infection with 10,000 Haemonchus contortus infective larvae (L3). Enzyme-linked immunosorbent assay was carried out in serum samples to determine the level of IgG, IgA and IgE anti-H. contortus L3 crude extracts and adult excretion/secretion products (ESP). Parasitological and blood immunological parameters were measured on the 2 extreme groups. Despite the absence of any typical signs of haemonchosis, susceptible kids had more than 11 times higher faecal egg counts (FEC) at 35 days post-infection (d.p.i.) than resistant kids had. Levels of immunoglobulin against H. contortus L3 and ESP increased significantly after infection in both groups. However, no difference in the host immune response mediated by immunoglobulin against H. contortus was evidenced between groups. This finding suggests that, in goats previously infected by H. contortus, a degree of protection occurred and the phenotypic and genetic segregation in resistant and susceptible animals were not related to the humoral immune response. The correlation coefficients between FEC and IgE anti-ESP (r = 0.593; P < 0.05 was significant in both resistant and susceptible animals. Such correlation suggesting a hypersensitivity reaction dependent on worm prolificacy has never been described. This result needs further studies to understand the mechanisms underlying this observation. #

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Section: Discussionmentioning

confidence: 71%

Serum antibody responses in Creole kids experimentally infected with Haemonchus contortus

Bambou¹,

Chevrotière²,

Varo³

et al. 2008

Veterinary Parasitology

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“…(49) and Kooyman et al. (8) and several studies have shown that sheep infected with GIN have increased total and worm‐antigen‐specific IgE (8,12,13,50,51). Mean serum IgE levels in our lambs exceeded 60 ng/mL through 16 days p.i.…”

Section: Discussionmentioning

confidence: 99%

Differences in immune parameters are associated with resistance to Haemonchus contortus in Caribbean hair sheep

MacKinnon¹,

Zajac²,

Kooyman³

et al. 2010

Parasite Immunology

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Caribbean hair sheep are more resistant to gastrointestinal nematodes than conventional wool breeds, but mechanisms that confer resistance are not fully understood. This study compared immune effector cell populations and antibody concentrations in 12 hair and 12 wool lambs infected with the abomasal parasite Haemonchus contortus and sacrificed at 3 or 27 days post-infection (p.i.) and 14 uninfected animals of each breed. Faecal egg counts were over 2.5-fold higher (P = 0.12) and packed cell volumes approximately 8% lower (P < 0.10) in infected wool lambs. Abomasal lymph nodes were heavier in infected animals (P < 0.05) and infected hair sheep had larger lymph nodes than infected wool sheep (P < 0.05). Tissue eosinophil concentrations were likewise larger (P = 0.07) in hair compared with wool sheep at 3 days p.i. Circulating levels of IgE and IgA in uninfected lambs were higher in hair sheep (P < 0.05) and during infection, hair sheep had higher serum IgA than wool sheep at 3, 5, and 21 days p.i. (P < 0.05). Serum IgE in infected lambs did not differ between breeds, but concentrations of IgE in lymph nodes were higher (P < 0.01) at 27 days p.i. in infected hair sheep.

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“…Host immunoglobulin (Ig)E responses have been associated with helminth infections for a long time (1) Studies on IgE responses in ruminants were restricted to sheep and cattle. Sheep IgE levels in serum or lymph increased after infection with gastrointestinal nematodes (2)(3)(4)(5)(6). In one of these studies (4), a correlation between total serum IgE and protection against Haemonchus contortus infection was found (4).…”

Section: Introductionmentioning

confidence: 99%

Serum immunoglobulin E response in calves infected with the lungworm Dictyocaulus viviparus and its correlation with protection

Kooyman

Yatsuda

Ploeger

et al. 2002

Parasite Immunology

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Protection of a primary Dictyocaulus viviparus infection was measured against a homologueous challenge infection in two independent experiments and this was correlated with serum immunoglobulin IgE responses. A primary infection of 30 third stage larvae (L3) of D. viviparus on day 0 protects calves for 70% against a challenge infection of 2000 L3 on day 35 compared to calves with no primary infection. The variation in post mortem worm counts within this group (n = 6) was very large with mean worm counts of 145 (range 3-446) lungworms. Parasite specific IgA, IgE, IgG1 and IgG2 and total IgE levels in serum were measured by ELISA. Parasite specific IgA, IgG1 and IgG2 were elevated after infection, but correlation with protection was only found with IgG1 levels on day 42 and with IgG2 levels on day 70. IgE was measured in a sandwich ELISA using antisheep IgE that cross-reacts with cattle IgE. No parasite specific IgE could be detected. However, total serum IgE was elevated after infection and total serum IgE levels before and on the day of challenge correlated with protection (P < 0.05). Total serum IgE also correlates with peripheral eosinophil counts between days 14 and 28 after primary infection. Western blots with three different parasite antigen preparations, L1, excretory/secretory products and crude worm adult antigens, were used to detect parasite specific IgE in sera depleted of IgG and IgM. These depleted sera from protected calves contained parasite specific IgE, while sera from nonprotected calves were negative. A band of approximately 100 kDa was recognized in all three antigens. In a second experiment, primary doses of 30, 60, 120, 240, 480 and 960 L3 of D. viviparus were used and necropsy was 11 days after challenge. This experiment confirmed the correlation between protection and total IgE levels before and on the day of challenge. The rapid and strong IgE responses in protected animals after such a low infection might be caused by the specific characteristics of the lungworm antigens or by the somatic migration of the worm and might be involved in the rapid development of protection against lungworm reinfections in cattle.

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IgE responses in the serum and gastric lymph of sheep infected with Teladorsagia circumcincta

Cited by 16 publications

References 25 publications

Serum antibody responses in Creole kids experimentally infected with Haemonchus contortus

Serum antibody responses in Creole kids experimentally infected with Haemonchus contortus

Differences in immune parameters are associated with resistance to Haemonchus contortus in Caribbean hair sheep

Serum immunoglobulin E response in calves infected with the lungworm Dictyocaulus viviparus and its correlation with protection

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