IGF2BP3 functions as a potential oncogene and is a crucial target of miR-34a in gastric carcinogenesis

Zhou, Yuhang; Huang, Tingting; Siu, Ho Lam; Wong, Chi Chun; Dong, Yujuan; Wu, Feng; Zhang, Bin; Wu, William; Cheng, Alfred S.L.; Yu, Jun; To, Ka Fai; Kang, Wei

doi:10.1186/s12943-017-0647-2

Cited by 123 publications

(100 citation statements)

References 60 publications

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“…For example, hypoxiainducible microRNA-224 promotes the GC cell growth, migration and invasion by directly targeting RASSF8 [15], but miR-34a can act as a tumor suppressor targeting IGF2BP3 in gastric carcinogenesis [16]. Besides, miR-16 and miR-939 were confirmed to be contributed to the chemotherapy response of GC [17,18].…”

Section: Discussionmentioning

confidence: 98%

miR-488 acts as a tumor suppressor gene in gastric cancer

Zhao

et al. 2016

Tumor Biol.

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Gastric cancer is one of the major causes of cancer mortality. Several microRNAs play a role in the tumor growth and invasion. However, the underlying molecular mechanism remains poorly understood. We detected the miR-638 expression levels in tumor samples and adjacent noncancerous tissues from 68 patients with gastric cancer as well as in the gastric cancer cell line SGC-7901 and SC-M1. The cell cycle was analyzed by flow cytometry, cell proliferation was observed by CCK-8 assay and cell invasion was detected using Transwell assay. MiR-638 was down-regulated in human GC tissues and its expression level was negatively correlated to TNM stage and lymph metastasis. In the cell lines, aberrant expression of miR-638 was related to the cell proliferation, cell cycle and invasion. We also found that SOX2 had a negative correlation with miR-638 in GC tissues, and miR-638 overexpression could decrease SOX2 expression level by directly binding the 3'-UTR of SOX2. in vitro, downregulating SOX2 by siRNA could counteract the effect of miR-638 inhibitor on GC cells proliferation and invasion. Our results demonstrate that miR-638 may play a pivotal role in the growth and invasion of GC.

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Section: Discussionmentioning

confidence: 98%

miR-488 acts as a tumor suppressor gene in gastric cancer

Zhao

et al. 2016

Tumor Biol.

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“…Protein concentration was quantified by the method of Bradford (Bio‐Rad, Hercules, CA). Detailed methods were described as a former report . PIEZO1 protein was then detected by anti‐PIEZO1 antibody (1:1000, 15939‐1‐AP, Proteintech).…”

Section: Methodsmentioning

confidence: 99%

“…Procedures of total RNA extraction was described previously. 24 PCR primer sequences were as follows: PIEZO1 (sense: 5′-GGA CTC TCG CTG GTC TAC CT-3′; anti-sense: 5′-GGG CAC AAT ATG CAG GCA GA-3′), B2M (sense: 5′-ACT CTC TCT TTC TGG CCT GG-3′; antisense: 5′-ATG TCG GAT GGA TGA AAC CC-3′). The PCR reaction was performed on 7500 Fast Real-Time System (Applied Biosystems).…”

Section: Isolation Of Total Rna and Quantitative Real-time Pcr Assaymentioning

confidence: 99%

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PIEZO1 functions as a potential oncogene by promoting cell proliferation and migration in gastric carcinogenesis

Zhang

Zhou

Huang

et al. 2018

Molecular Carcinogenesis

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Gastric cancer (GC) is one of the most common malignancies in Asian areas. PIEZO1 has been implied to regulate epithelial homeostasis to play an important role in physiological processes. It is also related to tumor initiation and progression. However, the role of PIEZO1 has not yet been explored in GC. The expression of PIEZO1 in GC cell lines and primary samples was determined by qRT-PCR and Western blot. The clinical correlation of PIEZO1 in GC was evaluated by immunohistochemistry on tissue microarray. The oncogenic role of PIEZO1 was demonstrated in gastric tumorigenesis through a series of functional assays, including cell proliferation, cell invasion, and flow cytometry analysis. Drug sensitivity was also assessed by PIEZO1 knockdown experiment. PIEZO1 exhibited an upregulation in most of the GC cell lines and primary samples compared with non-tumorous gastric epithelial tissues. Increase of PIEZO1 was associated with poor disease specific survival. PIEZO1 knockdown led to inhibitory effect by suppressing cell proliferation and invasion and inhibiting xenograft formation. Decreased PIEZO1 enhanced the sensitivity of Cisplatin or 5-FU treatment. Morphological alteration was also observed in siPIEZO1 treated cells. GTP-Rac1 showed accumulated activated form, while total-RhoA was decreased accompanied with PIEZO1 knockdown. In the present study, PIEZO1 is required for cell proliferation, migration and invasion to promote GC progression. PIEZO1 also maintains cellular morphology related to GC cell motility by regulating the activity of Rho GTPase family members. Our data not only suggested a novel prognostic marker, but also provided a useful clinical therapeutic target for GC.

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“…In the past decades, it has been reported that many RBPs were aberrantly changed in tumors, which affected the procession of mRNA into protein level, and then involved in carcinogenesis [20,21]. For example, AGO2 promotes tumor progression via involving into the oncogenic miR-19b biogenesis [22]; ADAR1 facilitates the tumorigenesis of PCa by regulating the expression and stability of the prostate cancer antigen 3 (PCA3) [23,24]; dysregulated expression of IGF2BP3 accelerates gastric carcinogenesis due to the silence of miR-34a [25]; QKI-5 regulates cell proliferation in lung cancer by changing cancer-associated alternative splicing [26].In general, these results demonstrate that the RBPs are closely associated with the progression of human tumors. Therefore, we collected PCa expression profiles and clinical information from the cancer genome atlas (TCGA) database.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a six-RNA binding proteins prognostic signature and candidate drugs for prostate cancer

Gao

Meng

Zhang

et al. 2020

Preprint

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AbstractThe dysregulation of RNA binding proteins (RBPs) play critical roles in the progression of several cancers. However, the overall functions of RBPs in prostate cancer (PCa) remain poorly understood. Therefore, we first identified 144 differentially expressed RBPs in tumors compared to normal tissues based on the TCGA dataset. Next, six RBP genes (MSI1, MBNL2, LENG9, REXO2, RNASE1, PABPC1L) were screened out as prognosis hub genes by univariate, LASSO and multivariate Cox regression and used to establish the prognostic signature. Further analysis indicated that high risk group was significantly associated with poor RFS, which was validated in the MSKCC cohort. Besides, patients in high risk group was closely associated with dysregulation of DNA damage repair pathway, copy number alteration, tumor burden mutation and low-respond to cisplatin (P < 0.001), bicalutamide (P < 0.001). Finally, three drugs (ribavirin, carmustine, carbenoxolone) were predicted using Connectivity Map. In summary, we identified a six-RBP gene signature and three candidate drugs against PCa, which may promote the individualized treatment and further improve the life quality of PCa patients.

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IGF2BP3 functions as a potential oncogene and is a crucial target of miR-34a in gastric carcinogenesis

Cited by 123 publications

References 60 publications

miR-488 acts as a tumor suppressor gene in gastric cancer

miR-488 acts as a tumor suppressor gene in gastric cancer

PIEZO1 functions as a potential oncogene by promoting cell proliferation and migration in gastric carcinogenesis

Development and validation of a six-RNA binding proteins prognostic signature and candidate drugs for prostate cancer

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