IgG4 Detection of Echinococcus granulosus Paramyosin Is a Useful Diagnostic Test for Human Hydatidosis

Moghadam, Zohreh Kazemi; Ghaffarifar, Fatemeh; Khalilpour, Akbar; Aziz, Farhanah Abdul; Saadatnia, Geita; Noordin, Rahmah

doi:10.1128/cvi.00019-13

Cited by 10 publications

(5 citation statements)

References 29 publications

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“…42 A previous study by our laboratory with E. granulosus protoscolex antigen showed an increased sensitivity for the detection of CE antigens when IgG4 was used compared with IgG. 45 The results of this study reaffirmed the high potential for an IgG4 assay to be used in CE diagnosis.…”

Section: Discussionsupporting

confidence: 71%

Lateral Flow Test Using Echinococcus granulosus Native Antigen B and Comparison of IgG and IgG4 Dipsticks for Detection of Human Cystic Echinococcosis

Khalilpour¹,

Sadjjadi²,

Moghadam³

et al. 2014

The American Society of Tropical Medicine and Hygiene

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Abstract. Cystic echinococcosis (CE) caused by infection with Echinococcus granulosus is of major concern for humans in many parts of the world. Antigen B was prepared from E. granulosus hydatid fluid, and Western blots confirmed eight batches showing a band corresponding to the 8-/12-kDa subunit with positive serum and no lowmolecular mass band ( 15 kDa) with negative serum. The batches were pooled and used to prepare lateral flow immunoglobulin G4 (IgG4) and IgG dipsticks. Diagnostic sensitivity was determined using serum samples from 21 hydatidosis patients, and diagnostic specificity was established using sera from 17 individuals infected with other parasites and 15 healthy people. IgG4 dipstick had a diagnostic sensitivity of 95% (20 of 21) and a specificity of 100% (32 of 32). The IgG dipstick had a sensitivity of 100% (21 of 21) and a specificity of 87.5% (28 of 32). Thus, both IgG and IgG4 dipsticks had high sensitivities, but IgG4 had greater specificity for the diagnosis of human CE.

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Section: Discussionsupporting

confidence: 71%

Lateral Flow Test Using Echinococcus granulosus Native Antigen B and Comparison of IgG and IgG4 Dipsticks for Detection of Human Cystic Echinococcosis

Khalilpour¹,

Sadjjadi²,

Moghadam³

et al. 2014

The American Society of Tropical Medicine and Hygiene

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“…Most frequently, this happens with other cestodes, in particular E. multilocularis (50–100% cross‐reactivity) . Better Sp has been reported with the detection of IgG 4 isotypes; however, Se is much decreased .…”

Section: The Immune Response Applied To the Diagnosis Of Cementioning

confidence: 98%

The intermediate host immune response in cystic echinococcosis

Tamarozzi

Mariconti²,

Neumayr

et al. 2016

Parasite Immunology

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Cystic echinococcosis (CE) is a chronic, complex and neglected zoonotic infection. In most cases, CE cysts and the intermediate host co-habit for a long time in the absence of symptoms and elicit very little inflammation. However, the immune interplay between the parasite and the host is complex, encompassing effective parasite-killing immune mechanisms implemented by the host, which in turn are modulated by the parasite. The immune response to the parasite has been exploited for the diagnosis of the disease and for the development of an effective vaccine to use in the natural intermediate host, but the mechanisms of parasite killing and immunomodulation are still unknown. Here, we reviewed the immune effector mechanisms and the strategies of immune evasion in the intermediate host.

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“…Regarding diagnosis through skeletal proteins, paramyosin has been identified as a useful antigen in Western blot studies using serum samples of patients with hydatid disease [ 137 ]. Immunoproteomics on extracts of different stages of H. diminuta showed the identification of some cytoskeleton proteins, including actin, paramyosin, tubulins, laminin, and filamin [ 138 , 139 ].…”

Section: Diagnosis Of Tapeworm Diseases and The Cytoskeletal Proteinsmentioning

confidence: 99%

What about the Cytoskeletal and Related Proteins of Tapeworms in the Host’s Immune Response? An Integrative Overview

et al. 2023

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Recent advances have increased our understanding of the molecular machinery in the cytoskeleton of mammalian cells, in contrast to the case of tapeworm parasites, where cytoskeleton remains poorly characterized. The pertinence of a better knowledge of the tapeworm cytoskeleton is linked to the medical importance of these parasitic diseases in humans and animal stock. Moreover, its study could offer new possibilities for the development of more effective anti-parasitic drugs, as well as better strategies for their surveillance, prevention, and control. In the present review, we compile the results of recent experiments on the cytoskeleton of these parasites and analyze how these novel findings might trigger the development of new drugs or the redesign of those currently used in addition to supporting their use as biomarkers in cutting-edge diagnostic tests.

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IgG4 Detection of Echinococcus granulosus Paramyosin Is a Useful Diagnostic Test for Human Hydatidosis

Cited by 10 publications

References 29 publications

Lateral Flow Test Using Echinococcus granulosus Native Antigen B and Comparison of IgG and IgG4 Dipsticks for Detection of Human Cystic Echinococcosis

Lateral Flow Test Using Echinococcus granulosus Native Antigen B and Comparison of IgG and IgG4 Dipsticks for Detection of Human Cystic Echinococcosis

The intermediate host immune response in cystic echinococcosis

What about the Cytoskeletal and Related Proteins of Tapeworms in the Host’s Immune Response? An Integrative Overview

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