IgM SEROLOGY FOR RUBELLA AND HUMAN PARVOVIRUS B19

Cohen, B. J.; Supran, E. M.

doi:10.1016/s0140-6736(87)91778-8

Cited by 10 publications

(2 citation statements)

References 5 publications

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“…False positive rubella IgM results can occur following infection with other viruses, namely parvovirus B19, Epstein-Barr virus, cytomegalovirus, and measles, which all can produce cross-reactive IgM [44][45][46][47][48]. The presence of rheumatoid factor (an anti-human immunoglobulin autoantibody most commonly of the IgM class) is also a common source of interference in IgM assays [49][50][51].…”

Section: False Positive Rubella Igmmentioning

confidence: 99%

Dilemmas and Pitfalls in Rubella Laboratory Diagnostics in Low Prevalence or Elimination Settings

Charlton

Severini

2016

Curr Treat Options Infect Dis

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The changing epidemiology of rubella in populations with either low prevalence levels, or where rubella elimination has been achieved, has challenged laboratory diagnostics. This review will discuss the challenges in identification of acute rubella and outline appropriate confirmatory testing, particularly for cases with low pretest probability. Implementation of pre-analytical screening processes can mitigate follow-up testing required to confirm false positive antibody results, and laboratories should consider not accepting requests for testing that do not have appropriate travel or clinical history. The role of laboratory surveillance will be examined, and challenges in determining protective immunity to rubella infection in light of waning rubella antibody levels in vaccinated populations will be explored. Special populations including prenatal women and the prevention of congenital rubella syndrome will be discussed in the context of low prevalence populations.

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Section: False Positive Rubella Igmmentioning

confidence: 99%

Dilemmas and Pitfalls in Rubella Laboratory Diagnostics in Low Prevalence or Elimination Settings

Charlton

Severini

2016

Curr Treat Options Infect Dis

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“…Rheumatoid factor does not usually interfere in these assays, but false positive results may occur in MACEIA if aggregated IgG in the enzyme-labeled antiserum complexes with bound anti-globulins (Kurtz and Malic, 1981). Sera with Epstein-Barr virus (EBV), cytomegalovirus, and parvovirus B19-specific IgM antibodies may give false positive results in anti-J.L-capture assays (Cohen and Supran, 1987;Gerna et ai., 1987b;Kurtz and Anderson, 1985;Morgan-Capner et aI., 1983b) and heat-inactivated sera may give false positive results in MACRIA if RF is present (Ronald et ai., 1985).…”

Section: Diagnostic Assaysmentioning

confidence: 99%

Togaviridae: Rubella Virus

Best¹,

O’Shea²

1988

Laboratory Diagnosis of Infectious Diseases Principles and Practice

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Disease: Rubella, congenital rubella. Etiologic Agent: Rubella virus, genus Rubivirus of Togaviridae. Clinical Manifestations: Asyptomatic infections are common; fever with rash; lymphadenitis; arthropathy and arthritis in adults. Congenital infection may result in fetal death and spontaneous abortion, live birth of severely malformed infant, an infant with minimal damage, or a healthy infant. Pathology: Primary site of virus replication is the nasopharyngeal mucosa, followed by spread of virus to local lymph nodes and a viremia 7 days after infection. The appearance of a rash may be caused by viral antigen-antibody complexes. During pregnancy, the virus infects the placenta and is then disseminated to all fetal tissues. Laboratory Diagnosis: IgG serology in paired serum specimens; IgM serology in a single serum. IgG serology is in large-scale use for immunity testing. Epidemiology: Worldwide distribution; endemic in temperate climates, with seasonal peaks during spring and early summer. In countries with successful childhood vaccination programs, major epidemics have been prevented.

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