IgMs produced by two acquired immune deficiency syndrome lymphoma cell lines: Ig binding specificity and VH-gene putative somatic mutation analysis [published erratum appears in Blood 1994 Aug 1;84(3):995]

Abstract

Two B-cell lines, 2F7 and 10C9, were established by single cell cloning from biopsies obtained from two acquired immune deficiency syndrome patients with Burkitt's lymphoma. Representation of the original tumors was verified by demonstration of (1) identical biallelic rearrangement of Ig genes for 2F7 and (2) shared idiotype for 10C9. Both cell lines displayed cell-surface Ig and secreted Ig (IgM lambda for 2F7, IgM kappa for 10C9). IgMs from both cell lines immunoprecipitated actin; in addition, 2F7 IgM lambda immunoprecipitated recombinant human immunodeficiency virus type 1 (HIV-1) gp 160. 2F7 IgM lambda did not react with other autoantigens (double-stranded and single-stranded DNA, actin, bovine serum albumin, IgG), whereas 10C9 IgM kappa reacted with human IgG. The 2F7 IgM heavy-chain variable region (VH) showed a 95% nucleotide homology with a previously sequenced VHIII germline gene, hv3019b9, whereas the 10C9 IgM VH showed a 95% homology with a previously sequenced VHIV germline gene, VH4.21. Use of minimally modified VH genes and demonstration of reactivity with chronically present antigens (ie, actin, HIV-1 gp 160, or human IgG) suggests that B cells in HIV-1-infected individuals proliferating in response to chronic antigenic stimulation may be at increased risk for lymphomagenesis.

Expression and Function of the Chemokine, CXCL13, and Its Receptor, CXCR5, in Aids-Associated Non-Hodgkin's Lymphoma

“…Two AIDS-NHL cell lines, 2F7 (Burkitt subtype) and R (diffuse large B cell lymphoma subtype), and the Epstein-Barr virus (EBV)-lymphoblastoid B cell line, 40102, were used in these studies [ 41 – 43 ]. All cell lines were cultured in standard media as previously described [ 42 ].…”

“…All cell lines were cultured in standard media as previously described [ 42 ]. Both AIDS-NHL cell lines are positive for the Epstein-Barr virus (EBV) and are not infected with HIV [ 41 – 43 ].…”

Incidence of potential glycosylation sites in immunoglobulin variable regions distinguishes between subsets of Burkitt's lymphoma and mucosa‐associated lymphoid tissue lymphoma

“…Iranian BL: AF068653‐AF068656, AF068662‐AF068672, AF068674 (Chapman et al , 1998b). Acquired immunodeficiency syndrome (AIDS)‐associated BL: L29113‐L29116 (Riboldi et al , 1994); X82081‐X82085 (Eclache et al , 1996); S71240 (Jain et al , 1994); AF003377‐AF003381 (Ikematsu et al, 2000); and two V H gene sequences from Ng et al (1994 ) . MALT lymphomas: U70087, U70093, U70100, U70107, U70115, U70123, AF461021‐AF461029 (Du et al , 1996a, b; and unpublished); U79581‐U79590 (Bahler et al , 1997); AF038447‐AF038450 (Bahler & Swerdlow, 1998); AF216816‐AF216829 (Miklos et al, 2000).…”

Nanoencapsulated rituximab mediates superior cellular immunity against metastatic B-cell lymphoma in a complement competent humanized mouse model

“…We have established a highly metastatic 2F7-BR44 lymphoma cell line obtained from a CNS lymphoma. 9 This cell line was subcloned from the human 2F7 Burkitt NHL cell line, which was retrieved from an patient with AIDS-lymphoma 19 and selected for its high rate of metastasis (100%) to the CNS. 9 The 2F7-BR44 cells were modified to express firefly luciferase ( Fluc ), which allows us to track both cancer spread and therapeutic efficacy through in vivo bioluminescence imaging.…”