IgY stability in eggs stored at room temperature or at +4°C

Nilsson, Elin; Stålberg, Johan; Larsson, Anders

doi:10.1080/00071668.2011.646951

Cited by 24 publications

(24 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, Larsson and Sjoquist [29] studied pure IgY and confirmed that it can be stored for more than 10 years in 40°C without any change in antibody activity. Such stability was sustained for as long as 6 months at room temperature or for 1 month at 37°C [28]. The stability of IgY gel after this study still displayed ELISA reactivity (OD 0.6) after storage for 30 days.…”

Section: Discussionmentioning

confidence: 65%

See 1 more Smart Citation

Effect of topical anti-Streptococcus mutans IgY gel on quantity of S. mutans on rats’ tooth surface

Bachtiar¹,

Afdhal

Meidyawati³

et al. 2016

Acta Microbiologica Et Immunologica Hungarica

View full text Add to dashboard Cite

This study aims to evaluate the effect of anti-Streptococcus mutans IgY gel on quantity of S. mutans on rats' tooth surface. Sprague Dawley rats were exposed intraorally with S. mutans Xc and were fed a caries-inducing diet 2000. The 24 rats were divided into four groups: group A had their teeth coated with IgY gel; group B received sterilized water as a control; group C had their teeth coated with IgY gel starting on the 29 th day; and group D had their teeth coated with a gel without IgY. Plaque samples were swabbed from the anterior teeth for S. mutans colony quantification, and saliva was collected to measure immunoreactivity by enzyme-linked immunosorbent assay. The results indicated that the quantity of S. mutans in rats treated with IgY gel showed significant difference compared with the controls. After coating with IgY anti-S. mutans gel, the mean immunoreactivity in rat saliva was higher than that of the no treatment group. In conclusion, topical application with anti-S. mutans IgY gel reduced the quantity of S. mutans on the tooth surface.

show abstract

Section: Discussionmentioning

confidence: 65%

“…The IgY gel was stored for 30 days at room temperature [28]. Then, its immunoreactivity was compared to that of pure IgY in water using ELISA.…”

Section: Discussionmentioning

confidence: 99%

Effect of topical anti-Streptococcus mutans IgY gel on quantity of S. mutans on rats’ tooth surface

Bachtiar¹,

Afdhal

Meidyawati³

et al. 2016

Acta Microbiologica Et Immunologica Hungarica

View full text Add to dashboard Cite

show abstract

“…24 IgYs were found to be stable during storage at room temperature or at 4°C for 6 months without losing their activity. 76 Furthermore, IgY antibodies can be stored for 5-10 years at 4°C without significant loss of their activity; they can even retain their activity after storage for 6 months at room temperature or for 1 month at 37°C. 77 IgYs are preferably stored at −20°C for long-term storage; storage at −70°C can cause up to 50% loss of their activity.…”

Section: Advantages Of Passive Immunization With Igymentioning

confidence: 99%

IgY antibodies for the immunoprophylaxis and therapy of respiratory infections

Abbas

El‐Kafrawy

Sohrab

et al. 2018

Human Vaccines & Immunotherapeutics

View full text Add to dashboard Cite

Emergence of drug resistance among the causative organisms for respiratory tract infections represents a critical challenge to the global health care community. Further, although vaccination can prevent disease, vaccine development is impeded by several factors. Therefore, novel approaches to treat and manage respiratory infections are urgently needed. Passive immunization represents a possible alternative to meet this need. Immunoglobulin Y antibodies (IgYs) from the yolk of chicken eggs have previously been used against bacterial and viral infections in human and animals. Their advantages include lack of reaction with mammalian Fc receptors, low production cost, and ease of extraction. Compared to mammalian IgGs, they have higher target specificity and greater binding avidity. They also possess remarkable pathogen-neutralizing activity in the respiratory tract and lungs. In this review, we provide an overview of avian IgYs and describe their potential therapeutic applications for the prevention and treatment of respiratory infections.

show abstract

“…Due to the long phylogenetic distance between avian and mammals, chickens can more efficiently produce antibodies against highly conserved mammalian proteins. This also requires much less antigen to prompt an effective immune response (Nilsson et al, 2012;Xu et al, 2011). Chickens, thus, can be considered as convenient, cost-effective, and rich sources of specific antibodies that have been applied successfully for immunodiagnostic, prophylactic, and therapeutic purposes in recent years.…”

Section: Introductionmentioning

confidence: 99%

Production, purification, and evaluation of quail immunoglobulin Y against Salmonella typhimurium and Salmonella enteritidis

Esmailnejad

Abdi-Hachesoo

Nasab

et al. 2019

Molecular Immunology

View full text Add to dashboard Cite

A B S T R A C TSalmonella species have been the major foodborne problems in food production systems, with Salmonella enterica serovars typhimurium (S. typhimurium) and enteritidis (S. enteritidis) being among the more common isolates. The oral administration of chicken egg yolk specific antibodies (IgYs) has been established as an efficient alternative for treatment and prevention of gastrointestinal pathogens including Salmonella. The present study was aimed to investigate the possible production of specific IgYs against Salmonella typhimurium and Salmonella enteritidis in quail egg yolks. Salmonella spp.-free female Japanese quails (Coturnix coturnix japonica) were intramuscularly immunized with formalin or heat-inactivated Salmonella immunogens (1.0 × 10 9 CFU/mL) emulsified with Freund adjuvants. Egg yolk IgYs were purified using ammonium sulfate precipitation method. Anti-Salmonella IgYs titer and specificity were determined using enzyme-linked immunosorbent assay (ELISA) and western blot analysis. Salmonella specific IgYs detected in the immunized quails were significantly higher than those of the control group, which confirmed the immunization procedure. Specific IgYs against S. typhimurium and S. enteritidis were identified in both groups immunized with heat or formalin-inactivated immunogens. However, formalin-inactivated immunogens induced relatively higher immune responses over the heat-inactivated ones. Quail anti-Salmonella IgYs showed a high specificity to their corresponding immunogens, with moderate crossreactivity to other members of Enterobacteriaceae family. Quail can be regarded as a valuable and inexpensive source for producing large-scale of specific antibodies that can be used for immunodiagnostic and immunotherapeutic purposes.

show abstract

IgY stability in eggs stored at room temperature or at +4°C

Cited by 24 publications

References 14 publications

Effect of topical anti-Streptococcus mutans IgY gel on quantity of S. mutans on rats’ tooth surface

Effect of topical anti-Streptococcus mutans IgY gel on quantity of S. mutans on rats’ tooth surface

IgY antibodies for the immunoprophylaxis and therapy of respiratory infections

Production, purification, and evaluation of quail immunoglobulin Y against Salmonella typhimurium and Salmonella enteritidis

Contact Info

Product

Resources

About