IL-13 over-expression in skin is not confined to IgE-mediated skin inflammation

Ploeg, Ingeborg van der; Tehrani, Mahmood Jeddi; Matuseviciene, Giedre; Wahlgren, C F; Fransson, Jessica; Scheynius, A.

doi:10.1046/j.1365-2249.1997.4671365.x

Cited by 25 publications

(17 citation statements)

References 29 publications

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“…IL-13 producing T cells, both CD4 + and CD8 + , were significantly reduced in lesional skin as compared to non-lesional psoriatic skin (Figure 4). This is in agreement with a previous study that demonstrated an eightfold decrease in IL-13 mRNA expression in non-lesional versus lesional psoriatic skin (van der Ploeg et al , 1997). Our findings suggest that IL-13 production may play a protective role in non-lesional psoriatic skin by down-regulating pathogenic IL-17 production.…”

Section: Discussionsupporting

confidence: 94%

CD8+ T Cells in the Lesional Skin of Atopic Dermatitis and Psoriasis Patients Are an Important Source of IFN-γ, IL-13, IL-17, and IL-22

Hijnen

Knol

Gent

et al. 2013

Journal of Investigative Dermatology

243

173

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Although CD4+ T cells are known to contribute to the pathology of atopic dermatitis (AD) and psoriasis, the role of CD8+ T cells in these diseases remains poorly characterized. The aim of this study was to characterize the cytokine production of T cells from AD and psoriasis skin. We found that CD4+ T cells isolated from AD skin were largely Th2-biased, in agreement with prior reports. However, we also observed large numbers of CD8+ T cells producing IL-13, IFN-γ and IL-22. We observed increased numbers of CD8+ T cells isolated from AD skin, and immunohistochemistry studies confirmed the presence of CD8+ T cells in the dermis and epidermis of AD skin lesions. Surprisingly, T cell cytokine production was similar in the lesional and non-lesional skin of patients with AD. T cells from psoriatic lesional skin predominantly produced IFN-γ, IL-17 and IL-22, in agreement with prior studies. However, in addition to Th17 cells, we observed high percentages of CD8+ T cells that produced both IL-22 and IL-17 in psoriatic skin lesions. Our findings demonstrate that CD8+ T cells are a significant and previously unappreciated source of inflammatory cytokine production in both AD and psoriasis.

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Section: Discussionsupporting

confidence: 94%

CD8+ T Cells in the Lesional Skin of Atopic Dermatitis and Psoriasis Patients Are an Important Source of IFN-γ, IL-13, IL-17, and IL-22

Hijnen

Knol

Gent

et al. 2013

Journal of Investigative Dermatology

243

173

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“…Although marked neutrophilia has been reported as a characteristic of both allergen-induced and anti-IgE-induced LPR in humans and dogs 13,15,16,41,52 it is not a typical feature of uninfected AD skin in either species. 11,35,53,54 However, the significance of this response can be difficult to evaluate when 'irrelevant' antigen challenges have not been included in experimental protocols. Our results suggest that the contribution of non-specific inflammatory responses within the LPR may be underestimated if only diluent controls are used.…”

Section: Discussionmentioning

confidence: 99%

A canine model of cutaneous late‐phase reactions: prednisolone inhibition of cellular and cytokine responses

Pucheu‐Haston

Shuster²,

Olivry

et al. 2005

Immunology

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SummaryImmunoglobulin E (IgE)-mediated late-phase reactions can be induced in atopic humans by intradermal injection of relevant allergens or anti-IgE antibodies. The histology of these reactions resembles that of naturally occurring atopic dermatitis. Strikingly similar responses can be induced in dogs, suggesting that a canine model could prove valuable for preclinical investigation of drugs targeting late-phase reactions. This study was designed to characterize the cellular, cytokine and chemokine responses after intradermal anti-IgE injection in untreated and prednisolone-treated dogs. Normal beagles were untreated or treated with prednisolone before intradermal injection of polyclonal rabbit anti-canine IgE or normal rabbit IgG. Biopsies were taken before injection and 6, 24 and 48 hr after injection. Samples were evaluated by histological and immunohistochemical staining, as well as by real-time quantitative polymerase chain reaction analysis. Dermal eosinophil and neutrophil numbers increased dramatically within 6 hr after injection of rabbit anti-canine IgE, and remained moderately elevated at 48 hr. The numbers of CD1c + and CD3 + mononuclear cells were also increased at 6 hr. The real-time quantitative polymerase chain reaction demonstrated marked increases in mRNA expression for interleukin-13 (IL-13), CCL2, CCL5 and CCL17. Levels of mRNA for IL-2, IL-4, IL-6 and IFN-c did not change within the limits of detection. Prednisolone administration suppressed the influx of neutrophils, eosinophils, CD1c + and CD3 + cells, as well as expression of IL-13, CCL2, CCL5 and CCL17. These data document the cytokine and chemokine responses to anti-IgE injection in canine skin, and they demonstrate the ability of the model to characterize the anti-inflammatory effects of a known therapeutic agent.

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“…The role of IL‐13 in psoriasis and PsA is yet to be elucidated. IL‐13 and IL‐4 are not detectable in the skin of patients with patients or controls (63,68). However, IL‐13 is increased in the synovial fluid of patients with PsA (69).…”

Section: Th2 Pathwaymentioning

confidence: 99%

Genetic variations associated with psoriasis and psoriatic arthritis found by genome-wide association

Duffin¹,

Woodcock²,

Krueger³

2010

Dermatologic Therapy

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Psoriasis and psoriatic arthritis are immune disorders with a complex polygenic basis. HLA-Cw6, which lies in the major histocompatibility region on chromosome 6, is considered the major genetic determinant of psoriasis. Recent genome-wide association studies have identified new variants outside of the MHC with relevance to the immunology of psoriasis. Variants in or near genes that encode subunits of cytokines (IL12B, IL23A) or cytokine receptors (IL23R) are interesting given that the gene product of IL12B, p40, is the target of a recently approved monoclonal antibody therapy for psoriasis (ustekinumab). Association with psoriasis and psoriatic arthritis has been found in TNFAIP3 and TNFIP1, ubiquitin ligases in the NF-kappaB pathway, and IL13, a Th2 cytokine. Copy number variation of human beta-defensin and late cornified envelope genes also associate with psoriasis. Many of these genetic variations also associate with immune disorders considered psoriatic co-morbidities, including Crohn's disease and diabetes.

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IL-13 over-expression in skin is not confined to IgE-mediated skin inflammation

Cited by 25 publications

References 29 publications

CD8+ T Cells in the Lesional Skin of Atopic Dermatitis and Psoriasis Patients Are an Important Source of IFN-γ, IL-13, IL-17, and IL-22

CD8+ T Cells in the Lesional Skin of Atopic Dermatitis and Psoriasis Patients Are an Important Source of IFN-γ, IL-13, IL-17, and IL-22

A canine model of cutaneous late‐phase reactions: prednisolone inhibition of cellular and cytokine responses

Genetic variations associated with psoriasis and psoriatic arthritis found by genome-wide association

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