IL-15 up-regulates iNOS expression and NO production by gingival epithelial cells

Yanagita, Manabu; Shimabukuro, Yoshio; Nozaki, Tomoyoshi; Yoshimura, Naoko; Watanabe, Junko; Koide, Hiroko; Terakura, Mami; Saho, Teruyuki; Takedachi, Masahide; Jang, Myoung Ho; Kiyono, Hiroshi; Murakami, Shinya

doi:10.1016/s0006-291x(02)02201-5

Cited by 23 publications

(19 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It has also been reported that IL-15 upregulates inducible NO synthase (iNOS) expression and NO production by RAW264.7 cells (25). A similar result has been reported for epithelial cells (37). Based on our results, it is likely that the NK/NKT-independent in vivo antiviral activity of IL-15 is mediated via IFN-␤ and/or TNF-␣.…”

Section: Discussionsupporting

confidence: 90%

NK and NKT Cell-Independent Contribution of Interleukin-15 to Innate Protection against Mucosal Viral Infection

Gill

Rosenthal

Ashkar

2005

J Virol

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 90%

NK and NKT Cell-Independent Contribution of Interleukin-15 to Innate Protection against Mucosal Viral Infection

Gill

Rosenthal

Ashkar

2005

J Virol

View full text Add to dashboard Cite

show abstract

“…Activation of ADP receptors (like the CaR, a GPCR coupled to G␣ q-11 ) has been reported to upregulate the level of iNOS mRNA in human gingival epithelial cells (22). In the same cells, additional studies showed that IL-15 enhanced iNOS expression at both the mRNA and protein levels, similar to our study (39). However, another study found that IL-4 and interferon (IFN)-␥ increased iNOS enzyme activity and protein expression in B cell chronic lymphocytic leukemia (B-CLL) cells during in vitro culture (19).…”

Section: Discussionsupporting

confidence: 89%

Calcium-sensing receptor activation induces nitric oxide production in H-500 Leydig cancer cells

Tfelt-Hansen

Ferreira²,

Yano³

et al. 2005

American Journal of Physiology-Endocrinology and Metabolism

View full text Add to dashboard Cite

Nitric oxide (NO) is a versatile second messenger. NO is produced by Leydig cells, where NO is a negative regulator of steroidogenesis. In cancer cells, NO is thought to have mutagenic and proliferative effects. We have previously shown that the calcium-sensing receptor (CaR) has promalignant effects in rat H-500 Leydig cancer cells, a model for humoral hypercalcemia of malignancy. Calcium, the major physiological ligand of the CaR, is a recognized intracellular cofactor in the process of NO production by virtue of its positive modulation of neuronal and endothelial nitric oxide synthase (NOS), but importantly, not of inducible (i) NOS activity. iNOS activity is regulated by changes in its expression level. Therefore, we investigated whether CaR activation changes iNOS expression. We found that high extracellular calcium (Ca[Formula: see text]) upregulates the level of mRNA for iNOS, whereas no change was seen in neuronal or endothelial NOS, as assessed by microarray and real-time PCR, respectively. The high Ca[Formula: see text]-induced iNOS upregulation was also detected by Northern and Western blotting. By quantitative real-time PCR, we showed that calcium maximally upregulates iNOS at 18 h. The effect of calcium was abolished by overexpression of a dominant-negative CaR (R185Q), confirming that the effect of Ca[Formula: see text] was mediated by the CaR. Cells treated with high calcium had higher NO production than those treated with low calcium, as detected with the NO-specific DAF2-AM dye. This was confirmed in single-cell fluorescence determinations using confocal microscopy. In conclusion, high calcium upregulates the levels of iNOS mRNA and protein as well as NO production in H-500 cells, and the effect of Ca[Formula: see text] on iNOS expression is mediated by the CaR.

show abstract

“…As the regulation of iNOS gene expression occurs at primarily transcriptional levels [7], IFN-␤ in combination with IL-15 may stimulate iNOS synthesis, thus enhancing NO release in macrophages. Recently, Yanagita et al [35] presented evidence that IL-15 upregulates iNOS expression and NO production in human gingival epithelial. This is consistent with our findings in murine macrophage RAW 264.7 cells.…”

Section: Discussionmentioning

confidence: 99%

IL-15 induces IFN-β and iNOS gene expression, and antiviral activity of murine macrophage RAW 264.7 cells

et al. 2004

View full text Add to dashboard Cite

IL-15 up-regulates iNOS expression and NO production by gingival epithelial cells

Cited by 23 publications

References 33 publications

NK and NKT Cell-Independent Contribution of Interleukin-15 to Innate Protection against Mucosal Viral Infection

NK and NKT Cell-Independent Contribution of Interleukin-15 to Innate Protection against Mucosal Viral Infection

Calcium-sensing receptor activation induces nitric oxide production in H-500 Leydig cancer cells

IL-15 induces IFN-β and iNOS gene expression, and antiviral activity of murine macrophage RAW 264.7 cells

Contact Info

Product

Resources

About